Modification of soybean growth and abiotic stress tolerance by expression of truncated ERECTA protein from Arabidopsis thaliana

Shanmugam, Sudha; Zhao, Shan; Nandy, Soumen; Srivastava, Vibha; Khodakovskaya, Mariya

doi:10.1371/journal.pone.0233383

Cited by 14 publications

(9 citation statements)

References 37 publications

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“…In addition, its role in abiotic stress tolerance has been described in Arabidopsis , rice, and tomatoes ( Masle et al, 2005 ; van Zanten et al, 2009 ; Villagarcia et al, 2012 ; Shen et al, 2015 ). Suppression of ER signaling in tomato and soybean was linked with drought tolerance, and its overexpression in rice with the heat tolerance ( Villagarcia et al, 2012 ; Shen et al, 2015 ; Shanmugam et al, 2020 ). The mechanism of heat tolerance was not clear, but drought tolerance was apparently associated with reduced transpiration surface in the plants ( Villagarcia et al, 2012 ; Shanmugam et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Suppression of ER signaling in tomato and soybean was linked with drought tolerance, and its overexpression in rice with the heat tolerance ( Villagarcia et al, 2012 ; Shen et al, 2015 ; Shanmugam et al, 2020 ). The mechanism of heat tolerance was not clear, but drought tolerance was apparently associated with reduced transpiration surface in the plants ( Villagarcia et al, 2012 ; Shanmugam et al, 2020 ). However, since ER suppression leads to diminished growth ( Torii et al, 2003 ; Shpak et al, 2004 ), this study investigated whether drought tolerance through ER suppression could be gained without disturbing desirable agronomic traits in the crop plants such as soybean.…”

Section: Discussionmentioning

confidence: 99%

“…Soybean transformations were carried out by the Iowa State University Plant Transformation Facility using the pTF101.1 vector (Addgene # 134770) harboring 8.0 kb Arabidopsis ER gene with a stop codon after the transmembrane domain and therefore lacking the kinase domain (Δ Kinase ) ( Shpak et al, 2003 ). The development of the transformation vector and the transgenic lines in Williams 82 background has been described earlier by Shanmugam et al (2020) .…”

Section: Methodsmentioning

confidence: 99%

“…The sequence similarity and protein domain conservation between AtER and the four soybean ER homologs (GmERs) suggest functional conservation between the well-characterized Arabidopsis ER and the newly identified soybean ER genes ( Du et al, 2017 ). Recently, Δ Kinase was transformed into soybean, and the resulting transgenic lines were analyzed for the phenotypic response ( Shanmugam et al, 2020 ). As seen in the tomato plants, the expression of this Δ Kinase in soybean also resulted in smaller plants and reduced leaf size and number as well as drought and salinity tolerance as tested in the seedlings or the young vegetative plants ( Shanmugam et al, 2020 ), confirming the functional role of Arabidopsis ER in soybean.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Δ Kinase was transformed into soybean, and the resulting transgenic lines were analyzed for the phenotypic response ( Shanmugam et al, 2020 ). As seen in the tomato plants, the expression of this Δ Kinase in soybean also resulted in smaller plants and reduced leaf size and number as well as drought and salinity tolerance as tested in the seedlings or the young vegetative plants ( Shanmugam et al, 2020 ), confirming the functional role of Arabidopsis ER in soybean.…”

Section: Introductionmentioning

confidence: 99%

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Suppression of ERECTA Signaling Impacts Agronomic Performance of Soybean (Glycine max (L) Merril) in the Greenhouse

2021

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The ERECTA (ER) family of genes, encoding leucine-rich repeat receptor-like kinase (RLK), influences complex morphological and physiological aspects of plants. Modulation of ER signaling leads to abiotic stress tolerance in diverse plant species. However, whether the gain in stress tolerance is accompanied with desirable agronomic performance is not clearly known. In this study, soybean plants potentially suppressed in ER signaling were evaluated for the phenotypic performance and drought response in the greenhouse. These plants expressed a dominant-negative Arabidopsis thaliana ER (AtER) called ΔKinase to suppress ER signaling, which has previously been linked with the tolerance to water deficit, a major limiting factor for plant growth and development, directly compromising agricultural production. With the aim to select agronomically superior plants as stress-tolerant lines, transgenic soybean plants were subjected to phenotypic selection and subsequently to water stress analysis. This study found a strong inverse correlation of ΔKinase expression with the agronomic performance of soybean plants, indicating detrimental effects of expressing ΔKinase that presumably led to the suppression of ER signaling. Two lines were identified that showed favorable agronomic traits and expression of ΔKinase gene, although at lower levels compared with the rest of the transgenic lines. The drought stress analysis on the progenies of these lines, however, showed that these plants were more susceptible to water-deficit stress as compared with the non-transgenic controls. The selected transgenic plants showed greater stomata density and conductance, which potentially led to higher biomass, and consequently more water demand and greater susceptibility to the periods of water withholding.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Suppression of ERECTA Signaling Impacts Agronomic Performance of Soybean (Glycine max (L) Merril) in the Greenhouse

2021

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An LTR retrotransposon insertion inside CsERECTA for an LRR receptor-like serine/threonine-protein kinase results in compact (cp) plant architecture in cucumber

et al. 2023

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The compact (cp) phenotype in cucumber (Cucumis sativus L.) is an important plant architecture-related trait with a great potential for cucumber improvement. In this study, we conducted map-based cloning of the cp locus, identi ed and functionally characterized the candidate gene. Comparative microscopic analysis suggested that the short internode in the cp mutant is due to fewer cell numbers. Fine genetic mapping delimited cp into an 8.8-kb region on chromosome 4 harboring only one gene, CsERECTA (CsER) that encodes a leucine rich repeat receptor-like kinase. The insertion of a 5.5-kb insertion of along terminal repeat retrotransposon in the 22 nd exon resulted in loss-of-function of CsER in the cp plant. Spatiotemporal expression analysis in cucumber and CsER promoter-driven GUS assays in Arabidopsis indicated that CsER was highly expressed in the stem apical meristem and young organs, but the expression level was similar in the wild type and mutant cucumber plants. However, CsER protein accumulation was reduced in the mutant as revealed by western hybridization. The mutation in cp also did not seem to affect self-association of CsER for formation of dimers. Ectopic expression of CsER in Arabidopsis was able to rescue the plant height of the loss-of-function AtERECTA mutant, whereas the compact in orescence and small rosette leaves of the mutant could be partially recovered. Transcriptome pro ling in the mutant and wild type plants revealed hormone biosynthesis/or signaling, and photosynthesis pathways associated with CsER-dependent regulatory network. Our work from the present study provides new insights for the use of cp in cucumber breeding. HighlightsThe cucumber compact (cp) plant architecture is due to a loss-of-function mutation inside the CsERECTA gene for a leucine rich repeat receptor-like kinase (LRR-RLK), which is caused by insertion of an LTR retrotransposon in its 22 nd exon.

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Genetic assessment of diverse rice germplasm for yield-related and stress tolerance traits

Shanmugam,

Samonte,

Sanchez

et al. 2024

Genet Resour Crop Evol

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The development of stress-resistant crops without compromising their yield and nutritional quality is one of the major challenges in plant biology. Plant breeders have made considerable effort in developing cultivars with high yield and tolerance to biotic and abiotic stresses. This research aimed to evaluate pre-breeding resources for favorable genes conferring good yield and resistance to biotic and abiotic stresses for rice varietal improvement. Two hundred forty diverse rice cultivars from top countries with which the United States imports and exports rice were selected from the Germplasm Resource Information Network (GRIN) collection, and these were characterized using molecular markers for various desirable traits. Among the cultivars, 16 were used as checks. The genes of various traits evaluated were grain number (Gn1a), panicle weight (WFP), cold stress tolerance (qSCT-11 and qCTS-12), anaerobic germination (AG1 and AG2), rice blast disease resistance (PiB, Pi-ta, Ptr, and Piz), wide compatibility (S5-n), and restoring ability (Rf4). Out of these 240 accessions, 192 have the Gn1a gene, 168 possess the qSCT-11 cold tolerance gene, 139 carry the Rf4 gene, 152 have the qCTS12 cold tolerance gene, 87 have blast resistance genes, and 44 possess the WC gene. None of the 240 accessions possess the WFP (Wealthy Farmer’s Panicle) gene. The selections with favorable genes can be used by breeders as potential donor parents for varietal improvement programs to produce new and promising cultivars with desired specific traits that may contribute towards alleviating global issues related to food security.

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Modification of soybean growth and abiotic stress tolerance by expression of truncated ERECTA protein from Arabidopsis thaliana

Cited by 14 publications

References 37 publications

Suppression of ERECTA Signaling Impacts Agronomic Performance of Soybean (Glycine max (L) Merril) in the Greenhouse

Suppression of ERECTA Signaling Impacts Agronomic Performance of Soybean (Glycine max (L) Merril) in the Greenhouse

An LTR retrotransposon insertion inside CsERECTA for an LRR receptor-like serine/threonine-protein kinase results in compact (cp) plant architecture in cucumber

Genetic assessment of diverse rice germplasm for yield-related and stress tolerance traits

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