Modification of spontaneous renal lesion of APA hamsters by streptozotocin‐induced diabetes

Abstract

Syrian hamsters of the APA strain (APA hamsters) develop spontaneous mesangial thickening in the renal glomeruli from an early age. They also develop focal and segmental glomerulosclerosis (FSG) at and after 6 months of age. In this study, histopathological, immunohistochemical and lectin histochemical examinations were conducted to clarify the modification of the spontaneous renal lesions of APA hamsters by streptozotocin(SZ)-induced diabetes. Histopathological analysis revealed that the expansion of the mesangial region was more prominent and the thickening of the glomerular basement membrane (GBM) was weaker in SZ-treated animals than in non-treated ones. Immunohistochemical analysis suggested that type IV collagen and laminin were involved in the expansion of the mesangial region and thickening of the GBM. In lectin histochemical analysis, podocytes, capillary endothelial cells, GBM and a part of mesangial region of SZ-treated animals were positive for RCA120 and GSL-I with neuraminidase-pretreatment although they were negative for these lectins in non-treated animals. These results suggest that the spontaneous glomerular lesion of APA hamsters is modified qualitatively and quantitatively by SZ-induced diabetes.

Effects of Endothelin Receptor Antagonists on the Progression of Diabetic Nephropathy

“…Most of the studies using the STZ model of diabetes reported an increased synthesis of both glomerular matrix proteins: collagen type IV and laminin [17, 34, 35, 36]. However, using the very sensitive computer-aided image analysis technique for quantification of matrix proteins as done in the present study, we were unable to demonstrate an increased laminin synthesis.…”

Amelioration of the β‐cell dysfunction in diabetic APA hamsters by antioxidants and AGE inhibitor treatments

“…APA hamsters show hyperlipidemia as well as hyperglycaemia, and develop diabetic complications similar to human diabetic diseases, such as atherosclerosis [17][18][19][20] and nephropathy [21,22]. In this diabetic animal model, probucol treatment ameliorates glucose intolerance and increases the insulin-positive area as a percentage of the whole pancreas area without a cholesterol-lowering effect [23].…”

Protective Effects of Probucol Treatment on Pancreatic .BETA.-cell Function of SZ-induced Diabetic APA Hamsters.

“…Syrian hamsters of the APA strain (APA hamsters) are known to enter a marked and continuous diabetic state after a single injection of SZ without severe toxic effects [13,23,47]. Studies have also demonstrated that APA hamsters show hyperlipidemia as well as hyperglycemia and develop diabetic complications similar to human diabetic diseases, such as atherosclerosis [14,[46][47][48] and nephropathy [13,16,23].…”

“…Studies have also demonstrated that APA hamsters show hyperlipidemia as well as hyperglycemia and develop diabetic complications similar to human diabetic diseases, such as atherosclerosis [14,[46][47][48] and nephropathy [13,16,23]. We recently showed that SZ-induced APA hamsters keep a diabetic condition for a long period, because resident β-cells in islets proliferate after SZ injection, but they degenerate and secretion of insulin is maintained at low levels [37].…”