Modification of the analysis of amino acids in pig plasma

Jonge, L.H. de; Breuer, M.

doi:10.1016/0378-4347(93)e0390-c

Cited by 15 publications

(6 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The rest of the blood was centrifuged ( 0°C, 3,000 × g, 10 min) and within 24 h the refrigerated plasma was analyzed for ammonia and urea (Test combination 542946, Boehringer, Mannheim, Germany) and a-amino nitrogen (Huntington, 1984). An aliquot of the plasma was stored at −80°C until it was analyzed for PAH (Eisemann et al, 1987) and free amino acids (De Jonge and Breuer, 1994). The latter were analyzed in plasma, sampled at 0555, and from 0700 onward at hourly intervals.…”

Section: Methodsmentioning

confidence: 99%

Effect of dietary neutral detergent fiber on ileal digestibility and portal flux of nitrogen and amino acids and on nitrogen utilization in growing pigs.

Lenis¹,

Bikker²,

Meulen³

et al. 1996

Journal of Animal Science

102

View full text Add to dashboard Cite

Two experiments were conducted to determine the effects of dietary NDF on apparent ileal and fecal digestibility and portal flux of nitrogen (N) and amino acids, and on N retention in growing pigs. In four equal portions (at 0600, 1200, 1800, and 2400) barrows on Treatment B received a basal diet, based on casein, cornstarch, and dextrose, at a feeding level of 2.6 times energy for maintenance. Barrows on Treatment B+NDF received an additional amount of 15% (wt/wt) of purified wheat bran NDF (pNDF). In Exp. 1, four ileally cannulated barrows (40 to 75 kg) were used in a crossover arrangement comprising two treatments and three periods. The addition of pNDF decreased ileal N digestibility from 94.1 to 88.9% (P < .001), whereas ileal digestibility of most amino acids was 2 to 5.5 percentage units lower (P < .001). Utilization of ileally digested N increased from 64 to 72% with the addition of pNDF, presumably because of the contribution of pNDF to the energy supply. In Exp. 2, three barrows (30 to 54 kg) fitted with catheters in the portal vein and the mesenteric vein and artery were used in a crossover arrangement comprising two treatments and five periods. Portal absorption of nutrients was derived by multiplying the porto-arterial plasma concentration differences by portal vein plasma flow. The pNDF did not significantly affect the absorption of ileally digested amino acids and the portal flux of ammonia and urea. The results showed that addition of NDF reduced amino acid digestibility, but not the portal flux of digested amino acids, and NDF energy presumably improved utilization of ileally digested amino acids.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of dietary neutral detergent fiber on ileal digestibility and portal flux of nitrogen and amino acids and on nitrogen utilization in growing pigs.

Lenis¹,

Bikker²,

Meulen³

et al. 1996

Journal of Animal Science

102

View full text Add to dashboard Cite

show abstract

“…Detection with UV-detection at 207 nm. Plasma concentrations of the other LNAAs were measured by HPLC as described by de Jonge and Breuer (1994).…”

Section: Physiological and Neurobiological Measurementsmentioning

confidence: 99%

Chronic increase of dietary l-tryptophan decreases gentle feather pecking behaviour

Hierden

Koolhaas

Korte

2004

Applied Animal Behaviour Science

View full text Add to dashboard Cite

Many studies show the involvement of the serotonergic (5-HT) system in the performance of abnormal behaviour in both human and animals. Recently, we showed that acute reduction of 5-HT turnover in the forebrain, increased gentle and severe feather pecking behaviour in chicks from a high (HFP) and low feather pecking (LFP) line of laying hens, suggesting that the performance of feather pecking behaviour involves low 5-HT neurotransmission.In the present study, we postulated that if low 5-HT is causally underlying feather pecking, increasing 5-HT turnover in the forebrain will decrease the development and performance of feather pecking. Augmentation of 5-HT neurotransmission in the brain was induced by chronically increasing dietary levels of the essential amino acid l-tryptophan (TRP) from which 5-HT is synthesised. From the age of 34 days, LFP and HFP chicks were fed a diet containing 2% TRP, whereas control birds of both lines were continuously fed with the normal rearing feed (0.16% TRP). From 35 days of age, litter was removed from the pens (10 pens/line-treatment) and all chicks (10 chicks/pen) were housed on a slatted floor until the end of the experiment. At 49 days of age, feather pecking behaviour was studied for 30 min. At 50 days of age baseline corticosterone, TRP and other large amino acids (LNAAs) were measured in the blood plasma of decapitated chicks (10 chicks per line-treatment). Furthermore, plasma corticosterone and central 5-HT turnover levels in response to manual restraint (5 min) were determined (10 chicks/line-treatment).For neither gentle nor severe feather pecking a significant line × treatment interaction was found. However, TRP treatment resulted in a significant [P = 0.02] overall decrease of the frequency of gentle feather pecking. For severe feather pecking a similar but not significant pattern was found. Significant line effects were found for gentle and severe feather pecking. HFP birds showed significantly higher levels of gentle and severe feather pecking behaviour than LFP birds [P < 0.001]. TRP treatment significantly increased the TRP/LNAA ratio in the plasma of the chicks. Furthermore, TRP treatment overall increased baseline and stress-induced levels of plasma corticosterone (although * Corresponding author. Tel.: +31-320-238171; fax: +31-320-239094. van Hierden et al. / Applied Animal Behaviour Science 89 (2004) [71][72][73][74][75][76][77][78][79][80][81][82][83][84] more pronounced in the LFP line). TRP supplementation significantly increased 5-HT turnover in the hippocampus and archistriatum and tended to do so in the remainder of the forebrain.The results confirm our hypothesis that feather pecking behaviour is triggered by low serotonergic neurotransmission, as increasing serotonergic tone, by increasing dietary TRP, decreases gentle feather pecking behaviour.

show abstract

“…This is avoided on addition of sulfosalicyclic acid followed by SPE. An increase was observed in the results for threonine, asparagine, glutamic acid, glutamine, glycine, alanine, valine and lysine, whereas those for phenylalanine and tryptophane showed a decrease with this modification 143 .…”

Section: Underivatized Analytesmentioning

confidence: 87%

Analytical Aspects of Amino, Quaternary Ammonium, Nitro, Nitroso and Related Functional Groups

Zabicky

Bittner

2009

Patai's Chemistry of Functional Groups

View full text Add to dashboard Cite

show abstract

Modification of the analysis of amino acids in pig plasma

Cited by 15 publications

References 22 publications

Effect of dietary neutral detergent fiber on ileal digestibility and portal flux of nitrogen and amino acids and on nitrogen utilization in growing pigs.

Effect of dietary neutral detergent fiber on ileal digestibility and portal flux of nitrogen and amino acids and on nitrogen utilization in growing pigs.

Chronic increase of dietary l-tryptophan decreases gentle feather pecking behaviour

Analytical Aspects of Amino, Quaternary Ammonium, Nitro, Nitroso and Related Functional Groups

Contact Info

Product

Resources

About