Modification of the erythrocyte membrane dielectric constant by alcohols

Orme, Frank W.; Moronne, Mario M.; Macey, Robert I.

doi:10.1007/bf01871902

Cited by 30 publications

(8 citation statements)

References 63 publications

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“…An enhancement of permeability much more pronounced for hydrophobic ions than for erythri-to1 is also observed in erythrocytes in the presence of n-alkanols [24,26,Deuticke,B.,unpublished results]. This similarity suggested to us similar underlying mechanisms.…”

Section: I992 Biochemical Society Transactionsmentioning

confidence: 61%

“…This similarity suggested to us similar underlying mechanisms. Stimulation by alcohols of the permeation of hydrophobic ions has been ascribed [26,271 to an increase of the dielectric constant of the hydrophobic membrane core following the admixture, to the apolar hydrocarbon phase, of the more polar alkanols. It seems probable that, following oxidative modification of the membrane, the lipid domain may become slightly more polar.…”

Section: I992 Biochemical Society Transactionsmentioning

confidence: 99%

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Erythrocyte membrane barrier function: role of bilayer polarity and skeletal proteins

Deüticke

Klonk

Haest

1992

Biochemical Society Transactions

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The barrier function of the erythrocyte membrane, which is a vital prerequisite for the cell's survival, has long been known to be lost reversibly under the influence of biaxial stress during osmotic lysis. The processes occurring, on a molecular level, during lysis are, however, still essentially unknown [ 1, 21.More recent studies have demonstrated that uniaxial stress, particularly after minor oxidative alteration of membrane constituents [ 3 , 41 induces cation leakiness, leading to cell swelling and finally to lysis driven by a colloid-osmotic imbalance. More advanced states of membrane chemical damage, in particular by mild or aggressive oxidants, produce major leakiness to ions and nonelectrolytes even in the absence of additional shear stresses. Since our first observation of leak formation in erythrocytes treated with the mild SH-oxidant, diamide [5,6], we have studied a number of other examples of chemically induced leakiness, e.g. by SH-specific oxidants [7], by 0,-derived reactive species [ 8lo], by photo-oxidation [ l l ] , or by tellurite [12], and also following electroporation [ 131.Leaks induced by diamide and other mild oxidants are reversible following treatment with reducing agents, e.g. dithioerythritol (DTE). Leak formation can be suppressed by pretreatment of the cells with low concentrations of the SH alkylating agent N-ethylmaleimide (NEM). These findings indicate the involvement of SH groups. Leaks formed by electroporation are essentially stable at

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Section: I992 Biochemical Society Transactionsmentioning

confidence: 61%

Section: I992 Biochemical Society Transactionsmentioning

confidence: 99%

Erythrocyte membrane barrier function: role of bilayer polarity and skeletal proteins

Deüticke

Klonk

Haest

1992

Biochemical Society Transactions

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“…As for the permeation of solutes by the solubilityediffusion mechanism (Finkelstein, 1987), Born energy is required to transfer charged particles from the high dielectric aqueous phase to the low dielectric membrane interior. Based on this theory, lipid bilayers are virtually impermeable to most ions, because the electrostatic energy of ions is much lower in a water medium with high dielectric constant (about 80) than in a typical bilayer with low dielectric constant (about 2) (Orme et al, 1988). As proposed previously (Kimura and Ikegami, 1985), the dielectric constant of mem-branes increases when their fluidity is increased.…”

Section: Discussionmentioning

confidence: 80%

Influence of membrane physical state on lysosomal potassium ion permeability

Wang

Zhang

2005

Cell Biology International

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Lysosomal permeability to potassium ions is an important property of the organelle. Influence of the membrane physical state on the potassium ion permeability of isolated lysosomes was assessed by measuring the membrane potential with bis(3-propyl-5-oxoisoxazol-4-yl)pentamethine oxonol and monitoring the lysosomal proton leakage with p-nitrophenol. The membrane fluidity of lysosomes was modulated by treatment with membrane fluidizer benzyl alcohol and rigidifier cholesteryl hemisuccinate. Changes in the membrane order were examined by steady-state fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene. The measurements of membrane potential and proton leakage demonstrated that the permeability of lysosomes to potassium ions increased with rigidification of their membranes by cholesteryl hemisuccinate treatment at 37 degrees C, and decreased with fluidization of their membranes by benzyl alcohol treatment at 2 degrees C. The changes in ion permeability could be recovered by fluidizing the rigidified membranes and rigidifying the fluidized membranes. The results suggest that the physical states of lysosomal membranes play an important role in the regulation of their K(+) permeability.

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“…Alcohols mimic the effects of heat shock -particularly the effects on fluidity and permeability of membranes and on heat shock protein synthesis -in many ways (Orme et al 1988;Petrov and Okorokov 1990;Meyer et al 1995). The fact that heat shock and ethanol elicit similar responses in the various second messenger systems may thus be due to similar effects on ion concentrations, as has been shown in mammalian cells (Skrandies et al 1997).…”

Section: Discussionmentioning

confidence: 94%

Heat shock effects on second messenger systems of Neurospora crassa

Kallies

Gebauer²,

Rensing³

1998

Archives of Microbiology

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Exposure of growing hyphae of Neurospora crassa to heat shock (44 degrees C) or ethanol (2.6 M) for 1 h induced a significant increase in the cAMP level, which reached a maximum approximately 2 min after the beginning of treatment and then decreased to control values despite continued heat or ethanol exposure. A 10-s heat shock or a 5-s ethanol shock also resulted in a transient cAMP increase 2 min after the pulse. Heat shock or ethanol treatment led to an increase in the amount of catalytic subunits of the cAMP-dependent protein kinase A in the nucleus almost synchronously with the increase of cAMP in the cytoplasm. The concentration of cGMP decreased a few seconds after the beginning of heat shock (44 degrees C) or ethanol treatment (2.6 M) to approximately 50% of the control level. Exposure to heat shock (44 degrees C, 1 h) led to an increase in the amount of inositol phosphates 0.5-2 min after the onset of heat shock. Thereafter, inositol phosphate levels dropped to control values despite continued heat exposure. Incubation of growing hyphae with cAMP or 8-Br-cAMP led to a two- to threefold increase of inositol phosphates 10-300 s after the beginning of incubation. Heat treatment furthermore caused a rapid release of calcium from vacuoles as determined by Fura-2 measurement of the calcium content released from isolated vacuoles. These heat-shock-dependent second messenger changes may play a role in the heat-shock-induced phase shifts of the circadian clock and heat-shock-induced conidiation.

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Modification of the erythrocyte membrane dielectric constant by alcohols

Cited by 30 publications

References 63 publications

Erythrocyte membrane barrier function: role of bilayer polarity and skeletal proteins

Erythrocyte membrane barrier function: role of bilayer polarity and skeletal proteins

Influence of membrane physical state on lysosomal potassium ion permeability

Heat shock effects on second messenger systems of Neurospora crassa

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