Modification of thermoplastic polyurethane nanofiber membranes by in situ polydopamine coating for tissue engineering

Abstract

To improve the interaction between cells and scaffolds, the appropriate surface chemical property is very important for tissue engineering scaffolds. In this work, the dopamine (DA) was first introduced into thermoplastic polyurethane (TPU) matrix to obtain TPU/DA nanofibers by electrospinning. Subsequently, the TPU@polydopamine (PDA) composite nanofibers with core/shell structure were fabricated by in situ polymerization of PDA. In comparison with TPU nanofibers, the uniformization of PDA coating layer on the surface of TPU/DA composite nanofibers significantly increased due to the addition of DA, which used as the active sites to guide the PDA particles accumulated along with the fiber direction. The hydrophilicity and water uptake ability of TPU@PDA composite nanofibers were larger than those of TPU nanofibers. The TPU@PDA composite nanofibers possess excellent comprehensive mechanical properties of high strength, stiffness, elasticity, and recoverability because of the hydrogen bonding occurrence between PDA and DA, as well as between PDA and TPU matrix. The attachment and viability of mouse embryonic osteoblasts cells (MC3T3‐E1) cultured on TPU@PDA composite nanofibers were obviously enhanced compared with TPU nanofibers. Those results suggested that the modified TPU@PDA composite nanofibers have superior mechanical and biological properties, which promoting them potentially useful for tissue engineering scaffolds.

Rational Design of Thermoresponsive Microgel Templates with Polydopamine Surface Coating for Microtissue Applications

“…To achieve this goal, we focus on the surface modification of the microgels using PDA, as schematically shown in Figure 1‐2, since PDA demonstrates cell adhesion‐promoting properties and adheres to a multitude of surfaces through physical binding forces. [ 20,21 ]…”

A Solvent System Involved Fabricating Electrospun Polyurethane Nanofibers for Biomedical Applications

“…DMF and THF were common solvents of TPU in previous studies [ 21 , 22 , 23 ]. In this study, the DMF/THF (5/5) solvent system was used as the control group.…”

“…The mixture was stirred using a magnetic bar until the solution was uniform and free of bubbles. Based on previous studies [ 21 , 22 ] DMF and THF mixed at the volume radio of 5:5 was used as the control group.…”

“…When the concentration of the solut concentration, polymer chains in the solution do not have eno difficult to form a Taylor cone or get electrospun fibers w electrospinning. Figure 2 shows SEM images of nanofibers p TCM/TFE and DMF/THF solvent systems with TPU concentrat nanofibers from TCM/TFE solvent show a smooth surface m distribution, while electrospun TPU nanofibers from DMF/THF sh DMF and THF were common solvents of TPU in previous studies [21][22][23]. In this study, the DMF/THF (5/5) solvent system was used as the control group.…”

“…TPU can be dissolved and fo stable TPU solution when VTCM/ VTFE is 8/2, 7/3, 6/4, 5/5, 4/6. These solution transparent during long-term storage, and this is beneficial to industrial productio [21][22][23]. DMF/THF (5/5) solvent system was used as the control group.…”