Modification of Threonine-1050 of SlBRI1 regulates BR Signalling and increases fruit yield of tomato

Wang, Shufen; Liu, Jianwei; Zhao, Tong; Du, Chenxi; Nie, Shaoping; Zhang, Yanyu; Lv, Siqi; Huang, Sunhua

doi:10.1186/s12870-019-1869-9

Cited by 24 publications

(25 citation statements)

References 45 publications

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“…The supernatants (100 μL) were subjected to ELISA according to the manufacturer’s instructions (MyBioSource). Optical density was measured at 450 nm using a microplate reader (Molecular Devices, Sunnyvale, CA, USA), as described previously [71].…”

Section: Methodsmentioning

confidence: 99%

Melatonin Deficiency Confers Tolerance to Multiple Abiotic Stresses in Rice via Decreased Brassinosteroid Levels

Hwang

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2019

IJMS

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Melatonin has long been recognized as a positive signaling molecule and potent antioxidant in plants, which alleviates damage caused by adverse conditions such as salt, cold, and heat stress. In this study, we found a paradoxical role for melatonin in abiotic stress responses. Suppression of the serotonin N-acetyltransferase 2 (snat2) gene encoding the penultimate enzyme in melatonin biosynthesis led to simultaneous decreases in both melatonin and brassinosteroid (BR) levels, causing a semi-dwarf with erect leaf phenotype, typical of BR deficiency. Here, we further characterized snat2 rice in terms of grain morphology and abiotic stress tolerance, to determine whether snat2 rice exhibited characteristics similar to those of BR-deficient rice. As expected, the snat2 rice exhibited tolerance to multiple stress conditions including cadmium, salt, cold, and heat, as evidenced by decreased malondialdehyde (MDA) levels and increased chlorophyll levels, in contrast with SNAT2 overexpression lines, which were less tolerant to stress than wild type plants. In addition, the length and width of grain from snat2 plants were reduced relative to the wild type, which is reminiscent of BR deficiency in rice. Other melatonin-deficient mutant rice lines with suppressed BR synthesis (i.e., comt and t5h) also showed tolerance to salt and heat stress, whereas melatonin-deficient rice seedlings without decreased BR levels (i.e., tdc) failed to exhibit increased stress tolerance, suggesting that stress tolerance was increased not by melatonin deficiency alone, but by a melatonin deficiency-mediated decrease in BR.

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Section: Methodsmentioning

confidence: 99%

Melatonin Deficiency Confers Tolerance to Multiple Abiotic Stresses in Rice via Decreased Brassinosteroid Levels

Hwang

Back

2019

IJMS

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“…Several bri1 -defective mutants of many species exhibit a semi-dwarf phenotype and decreased stem-leaf angles, which are recognized as indispensable attributes for intensive agriculture [ 15 , 19 , 48 ]. We previously reported that SlBRI1-overexpressing plants exhibited increased tomato yields, and dephosphorylated Thr-1050 of SlBRI1 could promote tomato yield by compacting plant architecture, as well as increasing fruit number and fruit weight, under normal conditions [ 16 , 25 ]. In the present investigation, the effects of Ser-1040 of SlBRI1 on tomato yield were distinct from those of Thr-1050.…”

Section: Discussionmentioning

confidence: 99%

“…Dephosphorylation of Thr-1054 in SlBRI1 severely attenuated BR signalling and disturbed plant growth in tomato [ 24 ]. Our previous study further found that the tomato SlBRI1 phosphorylation site Thr-1050 could affect tomato yield by regulating BR signalling [ 25 ]. These results suggest the potential value of phosphorylation sites of BRI1 in crop agronomic trait improvement, given their precise modification of the biological function of BRI1.…”

Section: Introductionmentioning

confidence: 99%

Modification of Serine 1040 of SIBRI1 Increases Fruit Yield by Enhancing Tolerance to Heat Stress in Tomato

Wang

Tian

et al. 2020

IJMS

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High temperature is a major environmental factor that adversely affects plant growth and production. SlBRI1 is a critical receptor in brassinosteroid signalling, and its phosphorylation sites have differential functions in plant growth and development. However, the roles of the phosphorylation sites of SIBRI1 in stress tolerance are unknown. In this study, we investigated the biological functions of the phosphorylation site serine 1040 (Ser-1040) of SlBRI1 in tomato. Phenotype analysis indicated that transgenic tomato harbouring SlBRI1 dephosphorylated at Ser-1040 showed increased tolerance to heat stress, exhibiting better plant growth and plant yield under high temperature than transgenic lines expressing SlBRI1 or SlBRI1 phosphorylated at Ser-1040. Biochemical and physiological analyses further showed that antioxidant activity, cell membrane integrity, osmo-protectant accumulation, photosynthesis and transcript levels of heat stress defence genes were all elevated in tomato plants harbouring SlBRI1 dephosphorylated at Ser-1040, and the autophosphorylation level of SlBRI1 was inhibited when SlBRI1 dephosphorylated at Ser-1040. Taken together, our results demonstrate that the phosphorylation site Ser-1040 of SlBRI1 affects heat tolerance, leading to improved plant growth and yield under high-temperature conditions. Our results also indicate the promise of phosphorylation site modification as an approach for protecting crop yields from high-temperature stress.

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“…In tomato, the BR biosynthetic gene DWF affects fruit yield by regulating architecture, and the BR level was found to be positively correlated with carotenoid accumulation in the fruits (Li et al, 2016). BR was found to promote tomato yield through enhanced autophosphorylation of SlBRI1, and increased plant expansion, leaf area, fruit weight, and number of fruits per cluster (Wang et al, 2019). Brassinolide (BL), 28homobrassinolide (28-hBL), and 24-epibrassinolide (24-eBL) treatments stimulated both the growth and yield of tomato plants (Vardhini and Rao, 2001).…”

Section: Regulation Of Yield By Brassinosteroid In Different Cropsmentioning

confidence: 99%

Designed Manipulation of the Brassinosteroid Signal to Enhance Crop Yield

Lin

2020

Front. Plant Sci.

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Brassinosteroid (BR), a plant steroid hormone, plays crucial role in modulating plant growth and development, which affect crop architecture and yield. However, BR application cannot highly benefit to agricultural production as expectation, because it regulates multiple processes in different tissues and leads to side effect. In addition, accurately modifying BR signal at transcriptional level is difficult. Effective manipulation of the BR signal and avoidance of side effects are required to enhance yield in different crops. Application of BR by spraying at specific developmental stages can enhance crop yield, but this method is impractical for use on a large scale. The accurate molecular design of crops would be much more helpful to manipulate the BR signal in specific organs and/or at particular developmental stages to enhance crop yield. This minireview summarizes the BR regulation of yield in different crops, especially horticultural crops, and the strategies used to regulate the BR signal to enhance crop yield. One popular strategy is to directly modulate the BR signal through modifying the functions of important components in the BR signal transduction pathway. Another strategy is to identify and modulate regulators downstream of, or in crosstalk with, the BR signal to manipulate its role in specific processes and increase crop yield. Efforts to accurately design a BR manipulation strategy will ultimately lead to effective control of the BR signal to avoid side effects and enhance crop yield.

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Modification of Threonine-1050 of SlBRI1 regulates BR Signalling and increases fruit yield of tomato

Cited by 24 publications

References 45 publications

Melatonin Deficiency Confers Tolerance to Multiple Abiotic Stresses in Rice via Decreased Brassinosteroid Levels

Melatonin Deficiency Confers Tolerance to Multiple Abiotic Stresses in Rice via Decreased Brassinosteroid Levels

Modification of Serine 1040 of SIBRI1 Increases Fruit Yield by Enhancing Tolerance to Heat Stress in Tomato

Designed Manipulation of the Brassinosteroid Signal to Enhance Crop Yield

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