Modifications and Degradation of High Density Lipoproteins

Ángel, A.; Fong, Bessie S.

doi:10.1007/978-1-4684-1262-8_4

Cited by 1 publication

(1 citation statement)

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“…Similar results were found for adrenocortical membranes. l 1 HDL also binds to muscle cells, but there are clear differences between HDL and LDL binding in that HDL is not internalized and LDL competes as effectively as HDL for HDL binding. This was not due to lipoprotein cross-contamination, since SDS-PAGE of the LDL and HDL preparations showed that the major protein components were apoprotein BlOO and apoproteins A and E, These results imply that LDL and HDL may interact with the same recognition site with multiple potential configurations capable of recognizing a variety of lipoproteins as proposed by Angel and Fong. There is a marked change in LDL-receptor binding when muscle cells differentiate.…”

Section: Discussionmentioning

confidence: 98%

The binding of lipoproteins to human muscle cells: Binding and uptake of LDL, HDL, and α‐tocopherol

Gurusinghe

Niese

Renaud³

et al. 1988

Muscle and Nerve

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The specific binding of low-density lipoprotein (LDL) to cells and its subsequent uptake into these cells is well documented, but little is known of the LDL binding and uptake by skeletal muscle. Lipoproteins are the major transporters of tocopherols, deficiencies of which have been associated with a number of muscle diseases of animals. Their possible implication in human muscle diseases prompted our investigation of LDL and high-density lipoprotein (HDL) binding and uptake into human muscle cells in culture. Cultured human muscle cells were used at both the myoblast and myotube stage. They were incubated with LDL or HDL which were labelled by protein iodination or with (3H) alpha-tocopherol and receptor binding and cell uptake characteristics established. LDL binds to both myoblasts and myotubes, but the binding affinity increases significantly with the more highly differentiated cells. This binding appears to be specific to LDL receptors. The LDL is taken into the muscle cell and protein is degraded, as with other types of cells. HDL also binds to muscle cells, but there is no evidence of internalization. alpha-Tocopherol is transferred to muscle cells from both LDL and HDL, but the transfer is not dependent on lipoprotein internalization. HDL is effective as a means of transport of alpha-tocopherol to muscle cells, but LDL appears to be about one order more effective.

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Section: Discussionmentioning

confidence: 98%