Modifications of carbohydrate residues in the sheep oviductal ampulla after superovulation

Desantis, Salvatore; Accogli, Gianluca; Silvestre, Frédéric; Binetti, F; Caira, Michele; Lacalandra, Giovanni Michele

doi:10.1016/j.theriogenology.2014.11.038

Cited by 4 publications

(7 citation statements)

References 79 publications

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“…As in the above analyzed oviductal segments, the ampulla of the preovulatory phase also expressed supranuclear Tn antigen (HPA) and an increase in cell surface sialic acids (MAL II, sPNA, sSBA). A high presence of sialoglycans has been revealed in the sheep oviductal ampulla [ 28 ] and in growth phase of the camel oviduct [ 36 ]. Jaffe et al [ 23 ] found the oviductal glycoprotein mRNA levels were elevated in the ampulla after estradiol treatment and in the late follicular stage of the menstrual cycle.…”

Section: Discussionmentioning

confidence: 99%

“…Glycans of oviductal epithelium play a key role in oviductal physiology and mammalian reproduction [ 9 , 26 , 27 ]. Changes in glycan patterns have been reported in the oviductal glycoproteins of several mammals due to sex hormone fluctuation as well as after experimental treatment by in vivo injection of estradiol (see [ 28 ] for references). This work contributes to the knowledge on the reproductive system of the Papio hamadryas by correlating the menstrual cycle stages with changes in the glycan expression of the oviduct.…”

Section: Introductionmentioning

confidence: 99%

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Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of Baboon Papio hamadryas during the Menstrual Cycle

Desantis

Albrizio

Lacitignola

et al. 2022

Animals

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The mammalian oviduct is a highly specialized structure where fertilization and early embryonic development occur. Its mucosal epithelium is involved in maintaining and modulating a dynamic intraluminal fluid. The oviductal epithelium consists of ciliated and non-ciliated (secretory) cells whose differentiation and activity are sex hormone-dependent. In this study, we investigated for the first time both the morphology and the glycan composition of baboon oviductal epithelium during the menstrual cycle. Oviducts were laparoscopically removed from 14 healthy adult female Papio hamadryas whose menstrual cycle phase was assessed based on the sex hormone levels and the vaginal cytology features. Histological investigations were carried out on fimbriae, infundibulum, ampulla, and isthmus separately fixed in 4% (v/v) paraformaldehyde, embedded in paraffin wax, and stained with hematoxylin-eosin for morphological analyses and using a panel of nine fluorescent lectins for glycoconjugate characterization. The histomorphological analysis revealed that in the entire oviduct (i) the ciliated and non-ciliated cells were indistinguishable during the follicular and luteal phases, whereas they were highly differentiated during the preovulatory phase when the non-ciliated cells exhibited apical protrusions, (ii) the epithelium height was significantly higher in the preovulatory phase compared to other menstrual phases, and (iii) the number of ciliated cells significantly (p ≤ 0.05) increased from the fimbriae to the infundibulum and progressively reduced in the other oviductal segments with the lower presence of ciliated cells in the isthmus. The glycan characterization revealed a complex and region-specific composition during the different phases of the menstrual cycle. It can be summarized as follows: (i) high-mannosylated N-linked glycans (Con A reactivity) were present throughout the oviductal epithelium during the entire menstrual cycle and characteristically in the apical protrusions of non-ciliated cells of the ampulla during the preovulatory phase; (ii) sialoglycans with α2,3-linked sialic acids (MAL II binding) were expressed along the entire oviductal surface only during the preovulatory phase, whereas α2,6-linked ones (SNA affinity) were also detected in the surface of the luteal phase, although during the preovulatory phase they were characteristically found in the glycocalyx of the isthmus cilia, and O-linked sialoglycans with sialic acids linked to Galβl,3GalNAc (T antigen) (KsPNA) and terminal N-acetylgalactosamine (Tn antigen) (KsSBA) were found in the entire oviductal surface during all phases of the menstrual cycle; (iii) GalNAc terminating O-linked glycans (HPA staining) were mainly expressed in the entire oviducts of the luteal and preovulatory phases, and characteristically in the apical protrusions of the isthmus non-ciliated cells of the preovulatory phase; and (iv) fucosylated glycans with α1,2-linked fucose (LTA reactivity) occurred in the apical surface of fimbriae during the luteal phase, whereas α1,3/4-linked fucose (UEA I binders) were present in the apical protrusions of the ampulla non-ciliated cells and in the apical surface of isthmus during the preovulatory phase as well as in the isthmus apical surface of follicular-phase oviducts. These results demonstrate for the first time that morphological and glycan changes occur in the baboon oviductal epithelium during the menstrual cycle. Particularly, the sex hormone fluctuation affects the glycan pattern in a region-specific manner, probably related to the function of the oviductal segments. The findings add new data concerning baboons which, due to their anatomical similarity to humans, make an excellent model for female reproduction studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of Baboon Papio hamadryas during the Menstrual Cycle

Desantis

Albrizio

Lacitignola

et al. 2022

Animals

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“…After fixation, tissues were washed and dehydrated in ethanol series, cleared in xylene, and embedded in paraffin wax. 4-mm thick sections were cut and, after dewaxing with xylene and hydration in an ethanol series of descending concentration, they were stained with Mayer's hematoxylin and eosin (to study the general morphology) or processed for lectin histochemistry [34].…”

Section: Tissue Preparationmentioning

confidence: 99%

“…Thus, the aim of this study was to investigate for the first time the glycan pattern of the lining epithelium of sheep oviductal isthmus and the changes induced by an effective superovulatory treatment [32,33]. The glycan pattern was investigated by lectin histochemistry, the most suitable in situ technique to detect cellular glycosylation and has been successfully used to characterize oligosaccharides in the oviduct of several mammals, including sheep [34].…”

Section: Introductionmentioning

confidence: 99%

Glycan profile of oviductal isthmus epithelium in normal and superovulated ewes

et al. 2016

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“…Examined specimens from group II revealed marked affection in the epithelial lining together with increased apoptosis in muocsa (mostly the ciliated epithelial cells). The presence of negative charges on the oviductal cilia glycocalyx is considered the principle of the electrostatic interaction between the cilia and oocytecumulus cell complexes as well as pickup and transport of oocyte [38] . The oviductal pathology could be attributed to the significant decrease in the circulating estrogen and progesterone, where the oviducts are dynamic tissues whose cellular components respond to hormonal changes.…”

mentioning

confidence: 99%

The effectiveness of resveratrol in protection against histological alterations induced by hyperprolactinemia in reproductive organs of female albino rats

Ismail

Yousry

2018

Egyptian Journal of Histology

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Introduction: Hyperprolactinemia (HPRL) commonly causes primary amenorrhea and reproductive disorders. Resveratrol (RES), a natural phenol, possesses antioxidant, anti-inflammatory and anti-apoptotic effects. However, its protective role on the histological structure of female reproductive organs in case of HPRL remains unclear. Aim of the work:This study aimed at evaluating the protective effect of RES against the HPRL induced by metoclopramide in female albino rats. Materials and Methods: Female rats that showed three regular estrus cycles (determined by vaginal smears) were divided into three groups. Group I was the control group. Group II rats were given oral metoclopramide (2 mg/Kg/day) for 28 consecutive days. Group III rats were given oral RES (20 mg/kg/day) for 28 days concurrently with metoclopramide. Serum superoxide anion (O 2 − ), prolactin (PRL), estradiol (E2), progesterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) were assessed, in addition to measurement of tissues tumor necrosis factor-alpha (TNF-α). Sections from uteri, oviducts and ovaries were stained with hematoxylin and eosin and caspase-3 immunohistochemical stain and were subjected to morphometric and statistical studies. Results: Group II showed significant increase in O 2 − , TNF-α and PRL, with significant decrease in the sex hormones. There were histological alterations in the uteri, oviducts and ovaries, with significant increased caspase-3 immunoexpression compared to the control. While, in group III, there was significant decrease in O 2 − , TNF-α and PRL, with significant increase in the sex hormones. The uteri, oviducts and ovaries showed minimal changes, with significant reduction in caspase-3 immunoreactivity compared to group II. Conclusion: Administration of resveratrol ameliorates the oxidative stress, inflammation, hormonal assay alterations, histological changes and apoptosis resulted from induced HPRL in female rats.

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Modifications of carbohydrate residues in the sheep oviductal ampulla after superovulation

Cited by 4 publications

References 79 publications

Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of Baboon Papio hamadryas during the Menstrual Cycle

Modification of Morphology and Glycan Pattern of the Oviductal Epithelium of Baboon Papio hamadryas during the Menstrual Cycle

Glycan profile of oviductal isthmus epithelium in normal and superovulated ewes

The effectiveness of resveratrol in protection against histological alterations induced by hyperprolactinemia in reproductive organs of female albino rats

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