Modifications of plasma proteome in long-lived rats fed on a coenzyme Q10-supplemented diet

Abstract

Dietary coenzyme Q 10 prolongs lifespan of rats fed on a PUFAn-6-enriched diet.Our aim was to analyze changes in the levels of plasma proteins of rats fed on a PUFAn-6 plus coenzyme Q 10 -based diet. This approach could give novel insights into the mechanisms of lifespan extension by dietary coenzyme Q 10 in the rat. Serum albumin, which decreases with aging in the rat, was significantly increased by coenzyme Q 10 supplementation both at 6 and 24 months. After depletion of the most abundant proteins by affinity chromatography, levels of less abundant plasma proteins were also studied by using 2D-electrophoresis and MALDI-TOF mass fingerprinting analysis. Our results have shown that lifelong dietary supplementation with coenzyme Q 10 induced significant decreases of plasma hemopexin, apolipoprotein H and interalpha-inhibitor H4P heavy chain (at both 6 and 24 months), preprohaptoglobin, fibrinogen γ-chain precursor, and fetuin-like protein (at 6 months), and alpha-1-antitrypsin precursor and type II peroxiredoxin (at 24 months). On the other hand, coenzyme Q 10 -supplementation resulted in significant increases of serine protease inhibitor 3, vitamin D-binding protein (at 6 months), and Apo A-I (at 24 months). Our results support a beneficial role of dietary coenzyme Q 10 decreasing oxidative stress and cardiovascular risk, and modulating inflammation during aging.

Dietary oil modifies the plasma proteome during aging in the rat

“…Interestingly, a significant number of protein changes identified in this work as the result of consumption of the VOO in comparison with the SO diet were also highlighted in a previous study focused on changes induced by dietary CoQ 10 supplementation of a diet containing SO compared to an SO alone diet (Santos-González et al 2007). The fact that a VOO diet and a CoQ 10 -supplemented SO diet increase the levels of proteins, such as serine protease inhibitor III, apolipoprotein A-I, or vitamin D binding protein, and decrease other proteins, such as hemopexin, prepohaptoglobin, fibrinogen gamma chain, alpha-2-HS glycoprotein, type II peroxiredoxin, inter-alpha inhibitor H4P heavy chain, or apolipoprotein H, when compared with the SO alone diet indicates that CoQ 10 and VOO may have similar effects on processes related to inflammation, blood coagulation, lipid metabolism, transport, and bone metabolism.…”

“…Animals and diets used here were as documented in previous works (Bello et al 2006;Santos-González et al 2007;Quiles et al 2010). Groups of ten male Wistar rats (Rattus norvegicus) initially weighing 80-90 g were housed under standard conditions and maintained on a 12-h light/12-h darkness cycle and at a temperature of 22±1°C.…”

Mediterranean diet supplemented with coenzyme Q10 induces postprandial changes in p53 in response to oxidative DNA damage in elderly subjects

“…Previous evidence showed that DNA damage plays a crucial role in the mechanisms of atherosclerosis (Bennett 2001), as well as other diseases related to aging. In the same line, studies carried out on rats fed with diets rich in polyunsaturated fatty acids demonstrated that CoQ supplements have a protective effect against oxidative damage (Thomas et al 1996;Santos-Gonzalez et al 2007). Thus, our findings demonstrate for the first time that the long-term consumption of the Med+CoQ diet induced less DNA damage in elderly subjects, with an intermediate effect for the Med diet, both diets producing less damage than the SFA diet.…”

“…Indeed, supplementation with exogenous CoQ has been shown to lead to an increase in the CoQ content of LDL, and a decrease of their peroxidizability (Stocker et al 1991;Thomas et al 1996). Studies in animal models have shown the protective effect of CoQ when supplemented in the diet (Bello et al 2005;Santos-Gonzalez et al 2007), in which supplementation attenuated oxidative alterations, with lower levels of DNA damage (Quiles et al 2004). However, to date there are no human studies, especially cross-randomized controlled trials, aimed to determine the antioxidant effects of CoQ during the postprandial phase associated with aging.…”

Proteomic investigation of the prefrontal cortex in the rat clomipramine model of depression

“…The proteins of homogenized PFC tissues were precipitated using chloroform and methanol [34]. Subsequently, samples (n = 4 in each group) were resuspended in lysis buffer, and were assayed for protein concentration using 2-D Quant Kit.…”