Modified Adamek's medium renders high yields of Metarhizium robertsii blastospores that are desiccation tolerant and infective to cattle-tick larvae

Iwanicki, Natasha Sant’Anna; Ferreira, Bruna de Oliveira; Mascarin, Gabriel Moura; Delalibera, Ítalo

doi:10.1016/j.funbio.2018.05.004

Cited by 27 publications

(28 citation statements)

References 34 publications

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“…Blastospores are thin-walled, pleomorphic, hydrophilic propagules which generally germinate faster than conidia [24]. Blastospores production use to be cheaper than conidial production because they can be produced in large amounts in liquid media, and in less time and space [22,25]. Under proper storage conditions, they may remain viable for up to 12 months [26,27].…”

Section: Introductionmentioning

confidence: 99%

UV‐B tolerances of conidia, blastospores, and microsclerotia of Metarhizium spp. entomopathogenic fungi

et al. 2020

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The aim of the present study was to analyze ten native Metarhizium spp. isolates as to their UV‐B tolerances. Comparisons included: different fungal propagules (conidia, blastospores, or microsclerotia [MS]); conidia in aqueous suspensions or in 10% mineral oil‐in‐water emulsions; and conidia mixed with different types of soil. The UV‐B effect was expressed as the germination of conidia or culturability of blastospores and MS relative to nongerminated propagules. Metarhizium anisopliae LCM S05 exhibited high tolerance as blastospores and/or MS, but not as conidia; LCM S10 and LCM S08 had positive results with MS or conidia but not blastospores. The formulations with 10% mineral oil did not always protect Metarhizium conidia against UV‐B. Conidia of LCM S07, LCM S08, and LCM S10 exhibited the best results when in aqueous suspensions, 24 h after UV‐B exposure. In general, conidia mixed with soil and exposed to UV‐B yielded similar number of colony forming units as conidia from unexposed soil, regardless the soil type. It was not possible to predict which type of propagule would be the most UV‐B tolerant for each fungal isolate; in conclusion, many formulations and propagule types should be investigated early in the development of new fungal biocontrol products.

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Section: Introductionmentioning

confidence: 99%

UV‐B tolerances of conidia, blastospores, and microsclerotia of Metarhizium spp. entomopathogenic fungi

et al. 2020

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“…Fungi were grown on a modified Adamek medium ( Iwanicki et al 2018 ) with the following nutritional composition per liter: 80 g yeast extract, 40 g cornsteep liquor (Sigma, St. Louis, USA), minerals, trace metals and vitamins adapted from Jackson’s medium ( Jackson et al 1997 ) at the following concentrations per liter: KH 2 PO 4 , 2.5 g; CaCl 2 0.2H 2 O, 1.0 g; MgSO 4 0.7H 2 O, 0.83 g; FeSO 4 0.7H 2 O, 0.3 g; CoCl 2 0.6H 2 O, 29.6 mg; MnSO 4 .H 2 O, 12.8 mg; ZnSO 4 0.7H 2 O, 11.2 mg; 0.2 mg each of thiamin, riboflavin, pantothenate, niacin, pyridoxamine, thioctic acid; and 0.02 mg each of folic acid, biotin, and vitamin B12. The medium was amended with 140g L -1 of glucose solutions that were autoclaved separately.…”

Section: Methodsmentioning

confidence: 99%

“…A pilot study was carried out to determine the moment of expressive mycelial growth without the presence of conidia in agar medium, to determine optimal time point but for RNA extraction. Then, mycelial hyphae were harvested from five-day old fungal cultures grown on modified Adamek agar media ( Iwanicki et al 2018 ). Hyphae were removed from the medium with a sterilized and pre-cooled spatula and placed immediately in an Eppendorf tube containing 1mL of TRIzol and kept on ice.…”

Section: Methodsmentioning

confidence: 99%

Comparative RNAseq Analysis of the Insect-Pathogenic Fungus Metarhizium anisopliae Reveals Specific Transcriptome Signatures of Filamentous and Yeast-Like Development

Iwanicki

Delalibera

Eilenberg

et al. 2020

G3 Genes|Genomes|Genetics

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The fungus Metarhizium anisopliae is a facultative insect pathogen used as biological control agent of several agricultural pests worldwide. It is a dimorphic fungus that is able to display two growth morphologies, a filamentous phase with formation of hyphae and a yeast-like phase with formation of single-celled blastospores. Blastospores play an important role for M. anisopliae pathogenicity during disease development. They are formed solely in the hemolymph of infected insects as a fungal strategy to quickly multiply and colonize the insect’s body. Here, we use comparative genome-wide transcriptome analyses to determine changes in gene expression between the filamentous and blastospore growth phases in vitro to characterize physiological changes and metabolic signatures associated with M. anisopliae dimorphism. Our results show a clear molecular distinction between the blastospore and mycelial phases. In total 6.4% (n = 696) out of 10,981 predicted genes in M. anisopliae were differentially expressed between the two phases with a fold-change > 4. The main physiological processes associated with up-regulated gene content in the single-celled yeast-like blastospores during liquid fermentation were oxidative stress, amino acid metabolism (catabolism and anabolism), respiration processes, transmembrane transport and production of secondary metabolites. In contrast, the up-regulated gene content in hyphae were associated with increased growth, metabolism and cell wall re-organization, which underlines the specific functions and altered growth morphology of M. anisopliae blastospores and hyphae, respectively. Our study revealed significant transcriptomic differences between the metabolism of blastospores and hyphae. These findings illustrate important aspects of fungal morphogenesis in M. anisopliae and highlight the main metabolic activities of each propagule under in vitro growth conditions.

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“…The manipulation of the liquid medium is an essential factor to obtain high concentrations of the desired propagule, in a short time and with the ability to resist the stabilization process (JARONSKI & MASCARIN, 2017). In just two days of growth, Iwanicki et al (2018) obtained a concentration of 5.8 x 10 8 blastospores ml -1 for an isolate of M. robertsii, which remained with high viability after the drying process.…”

Section: Discussionmentioning

confidence: 99%

Production of >i<Purpureocillium lilacinum>/i< and >i<Pochonia chlamydosporia>/i< by submerged fermentation, and their effects on >i<Tetranychus urticae>/i< and >i<Heterodera glycines>/i< through seed inoculation of common beans plants

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Modified Adamek's medium renders high yields of Metarhizium robertsii blastospores that are desiccation tolerant and infective to cattle-tick larvae

Cited by 27 publications

References 34 publications

UV‐B tolerances of conidia, blastospores, and microsclerotia of Metarhizium spp. entomopathogenic fungi

UV‐B tolerances of conidia, blastospores, and microsclerotia of Metarhizium spp. entomopathogenic fungi

Comparative RNAseq Analysis of the Insect-Pathogenic Fungus Metarhizium anisopliae Reveals Specific Transcriptome Signatures of Filamentous and Yeast-Like Development

Production of >i<Purpureocillium lilacinum>/i< and >i<Pochonia chlamydosporia>/i< by submerged fermentation, and their effects on >i<Tetranychus urticae>/i< and >i<Heterodera glycines>/i< through seed inoculation of common beans plants

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