Modified chitosan for effective renal delivery of siRNA to treat acute kidney injury

Tang, Weimin; Jogdeo, Chinmay M.; Panja, Subhash Chandra; Tang, Siyuan; Ding, Ling; Yu, Ao; Foster, Kirk W.; Dsouza, Del; Chhonker, Yashpal S.; Jensen-Smith, Heather; Jang, Hee‐Seong; Boesen, Erika I.; Murry, Daryl J.; Padanilam, Babu J.; Oupický, David

doi:10.1016/j.biomaterials.2022.121562

Cited by 39 publications

(23 citation statements)

References 82 publications

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“…Nanotechnology-based drug delivery systems have facilitated AKI therapy, such as early prevention, early diagnosis, drug delivery, and gene therapy. − In particular, stimulus-responsive polymeric drug delivery systems have attracted increasing attention for low toxicity, less side effects, prone accumulation into targeted tissues, and response to the disease microenvironment. − Liu et al synthesized a ROS-responsive prodrug based on chitosan-SS31 for AKI therapy. The prodrug presented ROS-responsive drug release leading to an enhanced therapeutic effect of SS31.…”

Section: Introductionmentioning

confidence: 99%

Dual-Responsive Curcumin-Loaded Nanoparticles for the Treatment of Cisplatin-Induced Acute Kidney Injury

et al. 2022

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Acute kidney injury (AKI) has been a global public health concern leading to high patient morbidity and mortality in the world. Nanotechnology-mediated antioxidative therapy has facilitated the treatment of AKI. Herein, a hierarchical curcumin-loaded nanodrug delivery system (NPS@Cur) was fabricated for antioxidant therapy to ameliorate AKI. The nanoplatform could respond to subacidic and reactive oxygen species (ROS) microenvironments. The subacidic microenvironment led to a smaller size (from 140.9 to 99.36 nm) and positive charge (from −4.9 to 12.6 mV), contributing to the high accumulation of nanoparticles. An excessive ROS microenvironment led to nanoparticle degradation and drug release. In vitro assays showed that NPS@Cur could scavenge excessive ROS and relieve oxidative stress in H2O2-induced HK-2 cells through reduced apoptosis, activated autophagy, and decreased endoplasmic reticulum stress. Results from cisplatin-induced AKI models revealed that NPS@Cur could effectively alleviate mitochondria injury and protect kidneys via antioxidative protection, activated autophagy, decreased endoplasmic reticulum stress, and reduced apoptosis. NPS@Cur showed excellent biocompatibility and low toxicity to primary tissues in mice. These results revealed that NPS@Cur may be a potential therapeutic strategy for efficiently treating cisplatin or other cause-induced AKI.

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Section: Introductionmentioning

confidence: 99%

Dual-Responsive Curcumin-Loaded Nanoparticles for the Treatment of Cisplatin-Induced Acute Kidney Injury

et al. 2022

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“…After 20 min of paraformaldehyde fixation, the frozen kidney slides were incubated with proteinase K for 10 min at 25 °C. Following that, TUNEL labeling was performed in accordance with the manufacturer’s protocol . TUNEL-positive cells (green fluorescence) were counted in six different fields of each sample at random.…”

Section: Methodsmentioning

confidence: 99%

“…Following that, TUNEL labeling was performed in accordance with the manufacturer's protocol. 62 TUNEL-positive cells (green fluorescence) were counted in six different fields of each sample at random. The ratio of TUNELpositive cells was calculated by dividing the number of apoptotic cells by the total number of cells in the field of view.…”

Section: 5mentioning

confidence: 99%

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic Acid Acetoxymethyl Ester Loaded Reactive Oxygen Species Responsive Hyaluronic Acid–Bilirubin Nanoparticles for Acute Kidney Injury Therapy via Alleviating Calcium Overload Mediated Endoplasmic Reticulum Stress

Wang

Yan

et al. 2022

ACS Nano

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Calcium overload is one of the early determinants of the core cellular events that contribute to the pathogenesis of acute kidney injury (AKI), which include oxidative stress, ATP depletion, calcium overload, and inflammatory response with self-amplifying and interactive feedback loops that ultimately lead to cellular injury and renal failure. Excluding adjuvant therapy, there are currently no approved pharmacotherapies for the treatment of AKI. Using an adipic dihydride linker, we modified the hyaluronic acid polymer chain with a potent antioxidant, bilirubin, to produce an amphiphilic conjugate. Subsequently, we developed a kidney-targeted and reactive oxygen species (ROS)-responsive drug delivery system based on the flash nanocomplexation method to deliver a well-known intracellular calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid acetoxymethyl ester (BAPTA-AM, BA), with the goal of rescuing renal cell damage via rapidly scavenging of intracellularly overloaded Ca 2+ . In the ischemia−reperfusion (I/R) induced AKI rat model, a single dose of as-prepared formulation (BA 100 μg•kg −1 ) 6 h post-reperfusion significantly reduced renal function indicators by more than 60% within 12 h, significantly alleviated tissular pathological changes, ameliorated tissular oxidative damage, significantly inhibited apoptosis of renal tubular cells and the expression of renal tubular marker kidney injury molecule 1, etc., thus greatly reducing the risk of kidney failure. Mechanistically, the treatment with BA-loaded NPs significantly inhibited the activation of the ER stress cascade response (IRE1-TRAF2-JNK, ATF4-CHOP, and ATF6 axis) and regulated the downstream apoptosisrelated pathway while also reducing the inflammatory response. The BA-loaded NPs hold great promise as a potential therapy for I/R injury-related diseases. continued...

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“…This ensures the colocalization of the cargos and overcomes the pharmacokinetic issues, maximizing the chances of achieving the highest synergistic efficacy. [84][85][86][87] Also, in cases where NAs are used to inhibit the expression of genes involved in the development of drug resistance; silencing of genes temporarily sensitizes the cells to the anticancer drug. This creates a short timeframe in which the drug is effective.…”

Section: Lipid-based Codelivery Systems For Drug-nucleic Acid Combina...mentioning

confidence: 99%

Advances in Lipid‐Based Codelivery Systems for Cancer and Inflammatory Diseases

Jogdeo

Panja

Kanvinde

et al. 2022

Adv Healthcare Materials

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Combination therapy targeting multiple therapeutic targets is a favorable strategy to achieve better therapeutic outcomes in cancer and inflammatory diseases. Codelivery is a subfield of drug delivery that aims to achieve combined delivery of diverse therapeutic cargoes within the same delivery system, thereby ensuring delivery to the same site and providing an opportunity to tailor the release kinetics as desired. Among the wide range of materials being investigated in the design of codelivery systems, lipids have stood out on account of their low toxicity, biocompatibility, and ease of formulation scale-up. This review highlights the advances of the last decade in lipid-based codelivery systems focusing on the codelivery of drug-drug, drug-nucleic acid, nucleic acid-nucleic acid, and protein therapeutic-based combinations for targeted therapy in cancer and inflammatory diseases.

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Modified chitosan for effective renal delivery of siRNA to treat acute kidney injury

Cited by 39 publications

References 82 publications

Dual-Responsive Curcumin-Loaded Nanoparticles for the Treatment of Cisplatin-Induced Acute Kidney Injury

Dual-Responsive Curcumin-Loaded Nanoparticles for the Treatment of Cisplatin-Induced Acute Kidney Injury

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic Acid Acetoxymethyl Ester Loaded Reactive Oxygen Species Responsive Hyaluronic Acid–Bilirubin Nanoparticles for Acute Kidney Injury Therapy via Alleviating Calcium Overload Mediated Endoplasmic Reticulum Stress

Advances in Lipid‐Based Codelivery Systems for Cancer and Inflammatory Diseases

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