Modified electrochemical aptasensor for ultrasensitive detection of tetracycline: In silico and in vitro studies

Naseri, Masoomeh; Niazi‎, Ali; Bagherzadeh, Kowsar; Konoz, Elaheh; Samadikhah, Hamid Reza

doi:10.1016/j.foodchem.2023.136195

Cited by 14 publications

(4 citation statements)

References 32 publications

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“…The obtained limit of detection (LOD) is 1 fg/mL using a 3SD/L method (SD is the standard deviation of blank, and L is the slope of the calibration curve). The LOD and detection time in the work have sufficient advantages compared with the most recently published fluorescence analysis methods, 3 colorimetric assays, 11,12 electrochemical analytical method, 39,40 and SERS sensor 41,42 (Table S1).…”

Section: Enrichment Mechanismmentioning

confidence: 97%

Aptamer-Modified Porous Anodized Aluminum Substrate for Rapid and Ultrasensitive Detection of Tetracycline via Surface Enhanced Raman Spectroscopy Couple with Electric Field Enrichment

Dai,

He,

Zhou

et al. 2023

ACS Appl. Nano Mater.

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Tetracycline (TC) has broad-spectrum antimicrobial activity and is low cost and widely used in animal disease treatment drugs and feed additives. However, the misuse of tetracycline causes serious damage to ecosystems and poses a major threat to human health. Therefore, the development of rapid and ultrasensitive TC detection methods is of great significance for the effective monitoring and prevention of TC pollution. Here, a surface enhanced Raman spectroscopy (SERS) sensor combined with electric field enrichment technology was developed for rapidly quantitative testing of TC. The SERS sensor was constructed by introducing Ag nanoparticles (AgNPs) and a Raman probe (4aminothiophenol, 4-ATP) on the porous anodized aluminum (PAA) membrane through hybridization of aptamer-2 (Apt-2) with aptamer-1 (Apt-1). In the presence of TC, the SERS tags (4-ATP/AgNPs/Apt-2) dissociate from this sensing platform, attributed to the stronger specific binding of TC to Apt-1, leading to a significant decrease in the Raman signal, which enables indirect quantitative analysis of TC. Based on the ion current property of the composite PAA membrane and the high activity and specificity of the SERS substrate, TC can be rapidly enriched in 1 min by electric field enrichment technology and identified by potable Raman spectrometer in 3 min. The improved SERS sensor allows for a real-time response to 1 fg/mL−1 ng/mL TC, and the limit of detection (LOD) is 1 fg/mL. More importantly, the SERS sensor can be used to detect TC in real food samples (such as milk and shrimp) and has excellent performance. The sensing strategy is very promising for mitigating food security risk.

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Section: Enrichment Mechanismmentioning

confidence: 97%

Aptamer-Modified Porous Anodized Aluminum Substrate for Rapid and Ultrasensitive Detection of Tetracycline via Surface Enhanced Raman Spectroscopy Couple with Electric Field Enrichment

Dai,

He,

Zhou

et al. 2023

ACS Appl. Nano Mater.

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“…The biosensor showed a wide linear range from 1.0 10 −17 to 1.0 × 10 −5 M and a low detection limit (2.28 × 10 −18 M). The proposed device su cessfully determined tetracycline residues in milk samples [49].…”

Section: Antibiotics and Electrochemical Detectionmentioning

confidence: 99%

Relevant Aspects in the Development of Electrochemical Aptasensors for the Determination of Antibiotics—A Review

da Silva,

Pereira

2023

Electrochem

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Aptamers are three-dimensional structures of DNA or RNA that present high affinity and selectivity to specific targets, obtained through in vitro screening. Aptamers are used as biological recognizers in electrochemical biosensors, the so-called aptasensors, providing greater specificity in recognizing the most diverse analytes. Electrochemical aptasensors have extremely relevant characteristics, such as high sensitivity, low cost compared to other biorecognizers such as antibodies, and excellent compatibility, being considered one of the most promising alternative methods in several areas, such as biomedical diagnosis and monitoring environmental contaminants. In this sense, the present work reviews the relevant aspects of methodologies based on electrochemical aptasensors and their applications in determining antibiotics, seeking to foster innovation in electrochemical biosensors.

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“…Biosensors have emerged as a promising alternative for quick and cost‐effective antibiotic measurement. While biosensors for aminoglycosides, macrolides, and tetracyclines are developed (Mehlhorn et al., 2018 ; Naseri et al., 2023 ; Yang et al., 2023 ), progress in detecting beta‐lactam antibiotics is limited. Detecting penicillins and cephalosporins is until now addressed through enzymatic methods, aptamers and whole‐cell biosensors (Au et al., 2020 ; Chinnappan et al., 2020 ; Lautenschläger et al., 2020 ; Valtonen et al., 2002 ; Xiao et al., 2016 ), but detecting carbapenems is challenging due to their complex structure, limited stability and low abundance (Codjoe & Donkor, 2017 ), presenting an ongoing challenge for accurate and affordable measurement.…”

Section: Introductionmentioning

confidence: 99%

A whole‐cell hypersensitive biosensor for beta‐lactams based on the AmpR‐AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20

Higuera‐Llantén,

Alcalde‐Rico,

Vasquez‐Ponce

et al. 2024

Microbial Biotechnology

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Detecting antibiotic residues is vital to minimize their impact. Yet, existing methods are complex and costly. Biosensors offer an alternative. While many biosensors detect various antibiotics, specific ones for beta‐lactams are lacking. To address this gap, a biosensor based on the AmpC beta‐lactamase regulation system (ampR–ampC) from Pseudomonas sp. IB20, an Antarctic isolate, was developed in this study. The AmpR–AmpC system is well‐conserved in the genus Pseudomonas and has been extensively studied for its involvement in peptidoglycan recycling and beta‐lactam resistance. To create the biosensor, the ampC coding sequence was replaced with the mCherry fluorescent protein as a reporter, resulting in a transcriptional fusion. This construct was then inserted into Escherichia coli SN0301, a beta‐lactam hypersensitive strain, generating a whole‐cell biosensor. The biosensor demonstrated dose‐dependent detection of penicillins, cephalosporins and carbapenems. However, the most interesting aspect of this work is the high sensitivity presented by the biosensor in the detection of carbapenems, as it was able to detect 8 pg/mL of meropenem and 40 pg/mL of imipenem and reach levels of 1–10 ng/mL for penicillins and cephalosporins. This makes the biosensor a powerful tool for the detection of beta‐lactam antibiotics, specifically carbapenems, in different matrices.

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Modified electrochemical aptasensor for ultrasensitive detection of tetracycline: In silico and in vitro studies

Cited by 14 publications

References 32 publications

Aptamer-Modified Porous Anodized Aluminum Substrate for Rapid and Ultrasensitive Detection of Tetracycline via Surface Enhanced Raman Spectroscopy Couple with Electric Field Enrichment

Aptamer-Modified Porous Anodized Aluminum Substrate for Rapid and Ultrasensitive Detection of Tetracycline via Surface Enhanced Raman Spectroscopy Couple with Electric Field Enrichment

Relevant Aspects in the Development of Electrochemical Aptasensors for the Determination of Antibiotics—A Review

A whole‐cell hypersensitive biosensor for beta‐lactams based on the AmpR‐AmpC regulatory circuit from the Antarctic Pseudomonas sp. IB20

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