Modified flotation method with the use of Percoll for the detection of Isospora suis oocysts in suckling piglet faeces

Karamon, Jacek; Ziomko, I.; Cencek, Tomasz; Sroka, Jacek

doi:10.1016/j.vetpar.2008.05.020

Cited by 13 publications

(13 citation statements)

References 9 publications

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“…For example, diarrhoeal faeces of suckling piglets contained a lot of fat fraction flowing to the surface of flotation liquid, which reduces significantly the visibility in the McMaster chamber during microscopic examination (Karamon et al 2008). In this case, the efficiency of detection can be increased by the use of centrifugation with Percoll.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the accuracy and precision of the McMaster method in detection of the eggs of Toxocara and Trichuris species (Nematoda) in dog faeces

Kochanowski¹,

Dąbrowska²,

Karamon³

et al. 2013

FOLIA PARASIT

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Abstract:The aim of this study was to determine the accuracy and precision of McMaster method with Raynaud's modification in the detection of the eggs of the nematodes Toxocara canis (Werner, 1782) and Trichuris ovis (Abildgaard, 1795) in faeces of dogs. Four variants of McMaster method were used for counting: in one grid, two grids, the whole McMaster chamber and flotation in the tube. one hundred sixty samples were prepared from dog faeces (20 repetitions for each egg quantity) containing 15, 25, 50, 100, 150, 200, 250 and 300 eggs of T. canis and T. ovis in 1 g of faeces. To compare the influence of kind of faeces on the results, samples of dog faeces were enriched at the same levels with the eggs of another nematode, Ascaris suum goeze, 1782. in addition, 160 samples of pig faeces were prepared and enriched only with A. suum eggs in the same way. the highest limit of detection (the lowest level of eggs that were detected in at least 50% of repetitions) in all McMaster chamber variants were obtained for T. canis eggs (25-250 eggs/g faeces). In the variant with flotation in the tube, the highest limit of detection was obtained for T. ovis eggs (100 eggs/g). The best results of the limit of detection, sensitivity and the lowest coefficients of variation were obtained with the use of the whole McMaster chamber variant. There was no significant impact of properties of faeces on the obtained results. Multiplication factors for the whole chamber were calculated on the basis of the transformed equation of the regression line, illustrating the relationship between the number of detected eggs and that of the eggs added to the sample. Multiplication factors calculated for T. canis and T. ovis eggs were higher than those expected using McMaster method with Raynaud modification.

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Section: Discussionmentioning

confidence: 99%

Analysis of the accuracy and precision of the McMaster method in detection of the eggs of Toxocara and Trichuris species (Nematoda) in dog faeces

Kochanowski¹,

Dąbrowska²,

Karamon³

et al. 2013

FOLIA PARASIT

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“…It is based on the separating of eggs from faecal material and concentrating them by means of a flotation fluid with an appropriate specific gravity. This is a good technique to use in initial surveys to establish which groups of parasites are present (Karamon, et al, 2008).…”

Section: Simple Test Tube Flotation 2 Simple Flotationmentioning

confidence: 99%

Attributable risk of Capillaria species in domestic pigeons (Columba livia domestica)

Qamar

Butt

Ehtisham-ul-Haque

et al. 2017

Arq. Bras. Med. Vet. Zootec.

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Fecal samples were collected from 120 domestic pigeons to determine the Attributable risk of Capillaria spp. The Capillaria spp. was observed in 64 out of 120 (51%) pigeons (70 males and 50 females) under this study. A total of 64 (39 males and 25 females) were found naturally infected with Capillaria spp. with infection percentage of 51% and 50% in males and females respectively. Qualitative examinations include the direct microscopy and faecal floatation while quantitative examination includes McMaster technique (worms load was calculated per gram of the faeces). Month wise Attributable risk showed that eggs of the worms were found to be abundant in the month of July during the present study (60% to 73%) because of high humidity. Very high and very low temperature is not suitable for the proper development of the eggs. Qualitative and quantitative examination revealed that Capillaria spp. was more prevalent in males (51%) than females (50%) but overall there was no significant difference (P>0.05) in the male and female because both individuals invest equal amount of energy in search of food and incubating the eggs. Different breeds of pigeons gave different Attributable risk in different months during the study. Groups of pigeons from different locations showed different variable Attributable risk. Areas with high humidity were more suitable for the development of eggs, which is the reason why higher Attributable risk was observed in Shahdara (75%) area of Lahore, Pakistan.Keywords: Attributable risk, Capillaria species, pigeons, Pakistan RESUMO Amostras de fezes foram coletadas de 120 pombos domésticos para determinar os fatores de risco de Capillaria spp. Capillaria spp. foi observado em 64 de 120 (51%) pombos (70 machos e 50 fêmeas) neste estudo. Um total de 64 (39 machos e 25 fêmeas) foram naturalmente infectados com Capillaria spp. sendo 51% em machos e 50% em fêmeas. Exames qualitativos incluem microscopia direta e suspensão de fezes, e exames quantitativos incluem a técnica McMaster (vermes são calculados por grama de fezes). O risco por mês demonstrou que ovos dos vermes foram encontrados em abundância no mês de

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“…Its use has been described in detail in many diagnostics, both animal (Coles et al 1992;Karamon et al 2008;Morgan et al 2005;Nichols and Obendorf 1994;Pereckiene et al 2007;Rinaldi et al 2007;Ward et al 1997) and human (Bondarenko et al 2009;Flohr et al 2007;Stephenson et al 1989). Different McMaster method modifications use various weight of faeces examined, volumes and types of flotation solutions, sample dilutions, flotation times, applications of additional centrifugation, durations and speeds of centrifugation, numbers of sections of the McMaster slide counted and different coefficients for interpretation (Cringoli et al 2004;Karamon et al 2008;Pereckiene et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Which McMaster egg counting technique is the most reliable?

et al. 2011

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This study focuses on the comparison of three selected modifications of the McMaster counting technique, namely the McMaster method modified by Wetzel (W) and Zajíček (Z), as well as the concentration McMaster technique according to Roepstorff and Nansen (R&N). These modifications differ in the weights of faeces examined (W, 2 g/Z, 1 g/R&N, 4 g), flotation solutions (W, NaCl/Z, MgSO(4) + Na(2)S(2)O(3)/R&N, NaCl + glucose), centrifugation (W, none/Z, 2,000 RPM for 2 min and 2,000 RPM for 1 min/R&N, 1,200 RPM for 5 min), number of McMaster chambers investigated (W, 3/Z, 2/R&N, 2), and multiplication factors used (W, 67/Z, 33/R&N, 20). To investigate the sensitivity and reliability of these methods, nematode eggs (Teladorsagia circumcincta) were used. Parasite elements are distributed through negative binomial distribution in naturally infected host faeces, and the number of parasite elements in a given amount of faeces sample is unknown to man. Therefore, we decided to prepare the exact number of eggs which were added to the parasite negative faeces; the faecal sample was then investigated. From this perspective, this is the first time a comparison of the McMaster methods has been so accurately investigated. This approach allows us to evaluate the real sensitivity and reliability of the tested method. As the findings of this study indicate, the highest sensitivity and reliability were obtained using the Roepstorff and Nansen modification. This McMaster modification is able to detect 20 eggs per sample (in 70% of samples). Concentrations of 200 and 500 eggs can be found in almost 100% of samples. Moreover, this method is simple, cheap and fast. For these reasons, we can recommend this method for routine veterinary practice.

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Modified flotation method with the use of Percoll for the detection of Isospora suis oocysts in suckling piglet faeces

Cited by 13 publications

References 9 publications

Analysis of the accuracy and precision of the McMaster method in detection of the eggs of Toxocara and Trichuris species (Nematoda) in dog faeces

Analysis of the accuracy and precision of the McMaster method in detection of the eggs of Toxocara and Trichuris species (Nematoda) in dog faeces

Attributable risk of Capillaria species in domestic pigeons (Columba livia domestica)

Which McMaster egg counting technique is the most reliable?

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