Modified gold nanoparticle colorimetric probe-based biosensor for direct and rapid detection of Mycobacterium tuberculosis in sputum specimens

Kooti, Sara; Kadivarian, Sepide; Abiri, Ramin; Mohajeri, Parviz; Atashi, Sara Naybandi; Ahmadpor, Hossein; Alvandi, Amirhooshang

doi:10.1007/s11274-023-03564-w

Cited by 11 publications

(2 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An assessment of gold nanoparticles for detecting bacteria and viruses has been previously reported. A nanoparticle-based method is used for detecting Helicobacter pylori with 98% sensitivity and specificity [ 23 ], Escherichia coli with a detection limit of 2.17 pM of target DNA [ 24 ], pirAvp toxin gene (causative agent of acute hepatopancreatic necrosis disease) at 20 fg/µL of shrimp genomic DNA [ 25 ], influenza virus with a detection limit of 10 ng/ml [ 26 ], Salmonella with 89.15% sensitivity and 99.04% specificity [ 27 ], Mycobacterium tuberculosis (MTB) with 96.6% sensitivity and 98.9% specificity, Mycobacterium tuberculosis complex (MTBC) with 94.7% sensitivity and 99.6% specificity [ 28 , 29 ], Pseudomonas syringae pathovars with a sensitivity as low as 15 ng/mL of genomic DNA [ 2 ], S. epidermidis with a detection limit of 20 ng/mL [ 18 ], and Klebsiella pneumoniae with a sensitivity of 1 pg/μL [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Colorimetric bacteria sensing of Pseudomonas aeruginosa using gold nanoparticle probes

Mousivand

Haddadi

Kamaladini

2023

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Background Due to the advantages of molecular methods over biochemical methods, the use of molecular methods for diagnosing nosocomial infections such as Pseudomonas can be an appropriate and rapid way to choose the right diagnosis and treatment of infection and prevent further complications caused by the infection. The present article provides a description of the development of a nanoparticle-based detection technique for sensitive and specific deoxyribonucleic acid-based diagnostic of Pseudomonas aeruginosa. Specific thiolated oligonucleotide probes for one of the hypervariable regions of the 16S rDNA gene were designed and applied for colorimetric detection of the bacteria. Results The results of gold nanoprobe-nucleic sequence amplification indicated the probe attached to gold nanoparticles in the presence of the target deoxyribonucleic acid. It caused aggregation of gold nanoparticles in the form of connected networks resulting in color change and indicating the presence of the target molecule in the sample, which could be observed by the naked eye. In addition, the wavelength of gold nanoparticles changed from 524 to 558 nm. Multiplex polymerase chain reactions were performed using four specific genes of Pseudomonas aeruginosa (oprL, oprI, toxA, and 16S rDNA). The sensitivity and specificity of the two techniques were assessed. According to the observations, the specificity of both techniques was 100%, and the sensitivity was 0.5 ng/μL and 0.01 ng/μL of genomic deoxyribonucleic acid for multiplex polymerase chain reaction and colorimetric assay, respectively. Conclusions The sensitivity of colorimetric detection was about 50 times higher than the polymerase chain reaction using the 16SrDNA gene. The results of our study proved to be highly specific with potential use for early detection of Pseudomonas aeruginosa.

show abstract

Section: Discussionmentioning

confidence: 99%

Colorimetric bacteria sensing of Pseudomonas aeruginosa using gold nanoparticle probes

Mousivand

Haddadi

Kamaladini

2023

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

show abstract

“…In the past few years, several visual (naked eye) detection methods to detect MTBC have been researched, such as gold nanoparticles, − magnetic bead-based flocculation, − and lateral flow assays. − These methods provide rapid, user-friendly, and easy-to-interpret results without the need for specialized equipment or training, making them suitable for resource-poor or POC settings or during outbreaks, where timely diagnosis is critical. These methods can be tailored to work with various clinical specimen types, enhancing their versatility in detecting pathogens from different sources, such as sputum, blood, urine, or cerebrospinal fluid.…”

mentioning

confidence: 99%

Label-Free Citrate-Stabilized Silver Nanoparticles-Based, Highly Sensitive, Cost-Effective, and Rapid Visual Method for the Differential Detection of Mycobacterium tuberculosis and Mycobacterium bovis

Patnaik,

Dey

2023

ACS Infect. Dis.

View full text Add to dashboard Cite

Tuberculosis poses a global health challenge, and it demands improved diagnostics and therapies. Distinguishing between Mycobacterium tuberculosis (M. tb) and Mycobacterium bovis (M. bovis) infections holds critical "One Health" significance due to the zoonotic nature of these infections and inherent resistance of M. bovis to pyrazinamide, a key part of the directly observed treatment, shortcourse (DOTS) regimen. Furthermore, most of the currently used molecular detection methods fail to distinguish between the two species. To address this, our study presents an innovative molecularbiosensing strategy. We developed a label-free citrate-stabilized silver nanoparticle aggregation assay that offers sensitive, cost-effective, and swift detection. For molecular detection, genomic markers unique to M. tb and M. bovis were targeted using species-specific primers. In addition to amplifying species-specific regions, these primers also aid the detection of characteristic deletions in each of the mycobacterial species. Post polymerase chain reaction (PCR), we compared two highly sensitive visual detection methods with respect to the traditional agarose gel electrophoresis. The paramagnetic bead-based bridging flocculation assay successfully discriminates M. tb from M. bovis with a sensitivity of ∼40 bacilli. The second strategy exploits citrate-stabilized silver nanoparticles, which aggregate in the absence of amplified dsDNA on the addition of sodium chloride (NaCl). This technique enables the precise, sensitive, and differential detection of as few as ∼4 bacilli. Our study hence advances tuberculosis detection, overcoming the challenges of M. tb and M. bovis differentiation and offering a quicker alternative to time-consuming methods.

show abstract

Ultra-sensitive colorimetric detection of SARS-CoV-2 by novel gold nanoparticle (AuNP)-assisted loop-mediated isothermal amplification (LAMP) and freezing methods

Alhamid,

Tombuloglu,

BenRashed

et al. 2024

Microchim Acta

View full text Add to dashboard Cite

Modified gold nanoparticle colorimetric probe-based biosensor for direct and rapid detection of Mycobacterium tuberculosis in sputum specimens

Cited by 11 publications

References 42 publications

Colorimetric bacteria sensing of Pseudomonas aeruginosa using gold nanoparticle probes

Colorimetric bacteria sensing of Pseudomonas aeruginosa using gold nanoparticle probes

Label-Free Citrate-Stabilized Silver Nanoparticles-Based, Highly Sensitive, Cost-Effective, and Rapid Visual Method for the Differential Detection of Mycobacterium tuberculosis and Mycobacterium bovis

Ultra-sensitive colorimetric detection of SARS-CoV-2 by novel gold nanoparticle (AuNP)-assisted loop-mediated isothermal amplification (LAMP) and freezing methods

Contact Info

Product

Resources

About