Modified H-Point Standard Addition Method and Logarithmic Function for the Spectrophotometric and Spectrodensitometric Determination of Hesperidin and Diosmin in Mixtures

Abstract

The similarity in the chemical structure of hesperidin and diosmin represents a difficulty in determining one or both of them when present together in pharmaceutical dosage forms. Different techniques were described in this article in order to determine each one of them without interference from the. other, or from other additives in the pharmaceutical dosage forms. 383 The first method utilized the zero-order and first-derivative techniques for the determination of hesperidin at 290 nm in which diosmin has minimum absorbance value.The H-point standard addition method (HPSAM) was applied successfully in a concentration range from 5-40 jig ml' 1 , sensitivity 5ug ml" 1 and detection limit 5ug.The HPSAM was also applied for the first derivative spectrophotometric determination of diosmin in the presence of hesperidin under the same concentration range, detection limit and sensitivity.The correlation coefficients(r) and regression equations for hesperidin and diosmin were found to be: 0.998, 0.994, respectively Y = 0.0226 x -0.0011 and Y = 0.2614x + 0.0643 for hesperidin and diosmin, respectively.The HPSAM was applied for the determination of hesperidin and diosmin in bulk powder mixture, in Daflon 500, and in Dioven tablets.The second method employed high performance thin layer chromatographic (HPTLC), plates for the separation of diosmin and hesperidin mixture or in pharmaceutical dosage forms, followed by spectrodensitometric measurement at 370nm.Linear relationships were obtained for hesperidin and diosmin in a concentration range from l-10pg/spot, detection limit 0.5ug/spot and sensitivity lug-The range of measurements was extended to 50ug/spot utilizing the logarithmic mode. The regression equations and correlation coefficients were calculated in each case. Application of the proposed spectrodensitometric method was made to a mixture of bulk powder, and tablet dosage form with several advantages over the other published techniques. Downloaded by [Emory University] at 09:41 05 August 2015 HESPERIDIN AND DIOSMIN IN MIXTURES 385

Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Hesperidin and Diosmin in the Pharmaceutical Dosage Form

“…The Literature Review. According to the literature survey, it was found that few analytical methods such as UV [1][2][3][4][5], Fluorometric method [6], LC-method [7], LC-MS method [8], HPLC method [9,10], capillary electrophoresis [11], simple colorimetric method [12], thin layer chromatography (TLC) [13], and adsorptive stripping voltammetry [14] were reported for hesperidin and Diosmin. To our knowledge, no study related to the UV spectrophotometric method for the simultaneous estimation of hesperidin and diosmin has been reported in literature.…”

A Sensitive Spectrophotometric Method for Determination of Diosmin Using Sodium Nitroprusside as a Chromogenic Reagent

“…These methods include voltammetry, [11] thin-layer chromatography, [12] high performance liquid chromatography (HPLC), [13,14] Fourier transform infrared spectrometry, [15,16] and spectrodensitometry. [17] The methods based on modern instrumental techniques are sensitive, i.e., by the HPLC method, 50 ng diosmin per injection could be detected. [13] Some of these methods require expensive instruments and maintenance.…”

“…Just one spectrophotometric method for determination of diosmin has been reported. [17] This is based on the derivative technique applied for the determination of diosmin and hesperidin in mixtures.…”

A Spectrophotometric Method for Diosmin Determination

“…Few analytical methods have been established for D determination and proposed for drug monitoring such as voltammetric [4], high performance liquid chromatography [5,6], thin-layer chromatography [7,8] or Fourier transform infrared spectrometry [9,10] and spectrophotometric methods [7]. Because there are sensitive, convenient and relatively inexpensive, most of visible spectrophotometric methods are preferred for pharmaceutical determination.…”