Modified Lipoprotein Lipase Catalyzes Ester Synthesis in Benzene

Abstract

Modified lipoprotein lipase catalyzed the synthesis of trilaurin from mono- or diacylglycerol and fatty acid and also the synthesis of ester from fatty acid and alcohol in benzene. In the ester synthesis reaction, the longer the chain length of fatty acid or alcohol, the higher the ester synthesis activity. The ester synthesis was competitively inhibited by fatty acids with branch of carbon chain at the position neighboring the carboxyl group. Other substrates including secondary and tertiary alcohols and carboxylic acids having benzene ring were tested and discussed in relation to the ester synthesis.

Solubilization of Biomaterials into Ionic Liquids

“…PEO modification had already proved to be an excellent way to make proteins soluble in nonaqueous solvents [14]. There are nevertheless some reports of enzymatic activities [15,16] and structure [17] determination of PEO-modified enzymes in homogeneous solutions of organic solvents. Although native heme proteins are insoluble and redox inactive in dehydrated PEO oligomers, these heme proteins have proved to be soluble and show redox activity even in PEO oligomers when they are chemically modified with PEO [18].…”

Ester Synthesis in Various Organic Solvents by Three Kinds of Lipase Preparations Derived from Pseudomonas fragi 22.39 B

“…The course of the ester synthesis was followed by a colorimetric method 9 ) using cetyl palmitate as a standard. Water contents of the organic solvents were 0.001",,0.02 (vjv) and those of lipase preparations were 5.4, 5.2, and 3.2% (wjw) for crude, pure, and PEG-modified, respectively.…”

“…At intervals of 10 min, 100 pI of the reaction mixture was taken out and 0.2 N sulfuric acid in benzene was added to it to stop the reaction, and the synthesized ester was extracted by the method of Inada. 9 ) The extracted ester was colorimetrically measured by the method of Snyder/Oj and the reaction curve of the degree of ester synthesis was drawn. The curves obtained by the reactions in various organic solvents were straight until an hour.…”

Optimized enzymatic synthesis of methyl benzoate in organic medium. Operating conditions and impact of different factors on kinetics

“…Among various esters present in flavors, methyl esters (Perraud, 1995) and esters from nonaliphatic acids are still hard to obtain in good yields by enzymatic methods (Takahashi, 1984). However, they are very valuable to consumers when they are produced by enzymatic synthesis.…”

“…BzOH was reported as a lipase inhibitor (Takahashi, 1984). Its solubilization in generally used media is the main problem because of its solid form, while aliphatic acids are liquid.…”