Modified Method for Purifying Rat Liver Branched-Chain α-Ketoacid Dehydrogenase Complex

Fujimura, Yukihiro; Muramatsu, Yuji; Akita, Keiichi; Bajotto, Gustavo; Shimomura, Yoshiharu

doi:10.1271/bbb.80744

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Inhibitory Effect Of Mcfas On Purified Bckdh-bdk Complex1

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2015

2021

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Cited by 2 publications

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“…Purified BCKDH-BDK complex was prepared from rat livers as described previously [26,27]; the specific BCKDC activity was 6.62 units/mg protein. The kinase reaction mixture contained 2 units of BCKDC per ml in 20 mmol/L Hepes-NaOH (pH 7.5), 1.5 mmol/L MgCl 2 , 2 mmol/L dithiothreitol, 1% Tween 20, 50 mmol/L K 2 HPO 4 , 0.5 mmol/L ATP, and various concentrations of MCFAs.…”

Section: Inhibitory Effect Of Mcfas On Purified Bckdh-bdk Complexmentioning

confidence: 99%