Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples

Panini, Nancy V.; Salinas, Eloy; Messina, Germán A.; Raba, Julio

doi:10.1016/j.foodchem.2010.09.035

Cited by 57 publications

(24 citation statements)

References 43 publications

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“…Subsequently, the developed system was integrated in microfluidic chips following the scheme displayed in Figure 4, where the simple channel layout of a double-T microchip was used to perform sequentially the immunoreaction and the enzyme reaction by applying a program of electric fields [38]. Another system for the determination of ZEA in feedstuffs samples has been recently reported using a microbiochip microfluidic immunosensor coupled with flow injection (FIA) with Au/SPEs as the working electrode showing good sensitivity and accuracy, and minimizing the use of expensive reagents [39]. Table 4 summarizes the main characteristics reported for the detection of biological toxins using electrochemical magnetoimmunosensors.…”

Section: Detection Of Biological Toxinsmentioning

confidence: 99%

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

2011

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Nowadays, detecting the presence of bioterrorism and biohazard agents in environmental and food samples is of great concern, due to their toxicity, and because many of them are prone to be used in terrorism attacks. The use of functionalized magnetic beads (MBs) in the development of electrochemical immuno-and genosensors has resulted in innovative and powerful detection strategies that may be applied to environmental, food and clinical analysis. This review describes current research on the combination of functionalized MBs with electrochemical detection for the development of magnetobiosensors applied to rapid, sensitive and specific detection of bioterrorism and biohazard agents.

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Section: Detection Of Biological Toxinsmentioning

confidence: 99%

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

2011

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“…[1][2][3][4][5][6][7] Microfluidic devices are capable of performing many different functions, including sample injection and separation, cell sorting and counting, polymerase chain reaction (PCR), species mixing, micro pumping, and microfluidic rectification. [8][9][10][11][12][13] Compared to their large-scale counterparts, miniaturized systems have many important advantages, including a reduced sample and reagent consumption, a more rapid response, an enhanced efficiency, a lower power consumption, an improved sensitivity, lower fabrication and operating costs, greater portability, and the potential for integration with other miniaturized devices.…”

Section: Introductionmentioning

confidence: 99%

“…Ezkerra et al 37 proposed a micropump comprising a deflectable diaphragm and a set of out-of-plane cantilever check valves. The experimental results showed that the micropump achieved a maximum flow rate of 177 ll/min given an effective area of 10 mm 2 , an actuating frequency of 6 Hz and a driving pressure of 200 kPa. Ni et al 38 presented a pneumatic PDMS-based micropump consisting of two in-plane low-leakage check valves.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic rectifier based on poly(dimethylsiloxane) membrane and its application to a micropump

Wang

Tsai

et al. 2013

Biomicrofluidics

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A microfluidic rectifier incorporating an obstructed microchannel and a PDMS membrane is proposed. During forward flow, the membrane deflects in the upward direction; thereby allowing the fluid to pass over the obstacle. Conversely, during reverse flow, the membrane seals against the obstacle, thereby closing the channel and preventing flow. It is shown that the proposed device can operate over a wide pressure range by increasing or decreasing the membrane thickness as required. A microfluidic pump is realized by integrating the rectifier with a simple stepper motor mechanism. The experimental results show that the pump can achieve a vertical left height of more than 2 m. Moreover, it is shown that a maximum flow rate of 6.3 ml/min can be obtained given a membrane thickness of 200 lm and a motor velocity of 80 rpm. In other words, the proposed microfluidic rectifier not only provides an effective means of preventing reverse flow but also permits the realization of a highly efficient microfluidic pump. V C 2013 AIP Publishing LLC.

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“…These methods, however, require expensive equipment, complicated cleanup procedures, preconcentration steps, and skilled operators. The determination of the ZEA is usually also carried out by immunochemical assays, [32,33] which could process many samples per day, but have some drawbacks such as their cost, availability limited to some mycotoxins, and cross-reactivity phenomena that give rise to false positive results. [34] In addition to these, it is possible to meet the analytical methods for the determination of ZEA by utilizing certain sophisticated instruments in screening the literature, as well.…”

Section: Introductionmentioning

confidence: 99%

Determination of Zearalenone by the Capillary Zone Electrophoresis-Uv Detection and Its Application to Poultry Feed and Cereals

Güray

Tunçel

Uysal

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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& A capillary zone electrophoretic method for the determination of zearalenone is described in this study. In the separation, a run buffer consisting of 20 mM sodium tetraborate at pH 9.0 with 15% acetonitrile applying 15 kV, injecting 10 s at 50 mbar was utilized. Phenobarbital was a suitable internal standard and the signals were recorded at 254 nm. Zearalanon and internal standard appeared at 5.46 min (RSD% 0.20) and 6.35 min (RSD% 0.18), respectively. The repeatability results are in the range of 0.01-1.58 for inter-day. The linearity was investigated in the range of 5.20 Â 10 À7 M to 7.86 Â 10 À5 M ZEA and it was linear fitting to the equation of [rPN ¼ 244803.8 C(M) À0.0348; r ¼ 0.9999]. The LOD and LOQ were calculated to be 2.70 Â 10 À8 M (8.25 lg=L) and 8.17 Â 10 À8 M (25 lg=L), respectively. Then, the method was successfully applied to the analysis of ZEA in poultry feeds, flour of maize, grain, and certain cereal samples such as fibrous biscuit, popcorn, and rice crisp.

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Modified paramagnetic beads in a microfluidic system for the determination of zearalenone in feedstuffs samples

Cited by 57 publications

References 43 publications

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

Magnetic Beads‐Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents

Microfluidic rectifier based on poly(dimethylsiloxane) membrane and its application to a micropump

Determination of Zearalenone by the Capillary Zone Electrophoresis-Uv Detection and Its Application to Poultry Feed and Cereals

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