Modified semi-synthetic diet for mass rearing of wax moth <i>Galleria mellonella</i> L.

Singh, J. P.; Rani, Seema; Rinni, Riazuddin; Manish,

doi:10.5958/0974-8172.2019.00133.0

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Methodsmentioning

confidence: 99%

“…To isolate nematodes, the technique of baiting with live insects of the greater wax moth (Galleria mellonella L.) (Lepidoptera: Pyralidae) was developed and used in the laboratory in the following research (Birah et al 2008). Greater wax moth (GWM) cultures were raised in 1-liter glass containers, kept at 28 °C, and fed an artificial diet (Birah et al 2008). The last instar larvae were heat-treated by soaking in water at 60 °C for 10 seconds before use to avoid spinning their silken cocoon and further development to the pupal stage.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of the Biostimulants of Microbiological Origin on the Entomopathogenic and Plant Parasitic Nematodes from Miscanthus × Giganteus Plantations

Stefanovska,

Skwiercz,

Pidlisnyuk

et al. 2024

Journal of Horticultural Research

View full text Add to dashboard Cite

This study aimed to evaluate the effect of biostimulants derived from Streptomyces avermitilis metabolites on entomopathogenic nematodes: Steinernema carpocapsae, Steinernema feltiae, and Heterorhabditis bacteriophora, obtained from the soil of several Miscanthus × giganteus plantations in 2020–2023. The nematodes were isolated, identified, and cultured using live insects (the greater wax moth Galleria mellonella). Three preparations containing biostimulants – Charkor, Stimpo, and Regoplant, were tested for compatibility with entomopathogenic and plant parasitic nematodes. Their effect on nematode survival was evaluated using the Petri dish test. The study showed that the effect of biostimulants on the survival of nematodes depended on the concentration of aversectin contained in the evaluated preparations. Stimpo and Regoplant had an adverse effect on plant parasitic nematodes. The highest G. mellonella mortality was observed at the higher dose of Charkor (0.4%), and the lowest at the lower dose of Regoplant (1.22%). The study showed that the virulence of the nematodes decreased after 48 hours of incubation in Regoplant and Stimpo solutions containing aversectin. The degree of interaction between nematodes depended on the nematode species, trophic preferences, and the concentration of active ingredients in the preparations. This conclusion is crucial for the selection of appropriate types of entomopathogenic nematodes and the application rate of plant parasitic nematodes in the assessment of their short- and long-term spread, persistence, and recycling in field conditions.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of the Biostimulants of Microbiological Origin on the Entomopathogenic and Plant Parasitic Nematodes from Miscanthus × Giganteus Plantations

Stefanovska,

Skwiercz,

Pidlisnyuk

et al. 2024

Journal of Horticultural Research

View full text Add to dashboard Cite

show abstract

“…An artificial diet was prepared for feeding the larvae of the wax moth while the adult moths were fed on 30% honey solution. The diet formulation was prepared following the method provided by Singh et al (2019), with slight modification. The ingredients used for making artificial diet included wheat flour, corn flour, milk powder, yeast, honey, glycerine, wheat bran, bees-wax, vitamin E capsule and Streptomycin sulphate.…”

Section: Rearing Wax Mothmentioning

confidence: 99%

Isolation and Identification of Entomopathogenic Fungi from Soils of Manipur (N-E India)

Beemrote,

Srinivasan,

Jeyarani

et al. 2023

IJARe

View full text Add to dashboard Cite

Background: Entomopathogenic fungi (EPF) are natural adversaries of insects, serving a crucial role in the regulation of insect pest populations. In response to the growing demand for sustainable agricultural practices that prioritize environmental protection, human safety and animal welfare, the utilization of bio-control agents like entomopathogenic fungi offers a superior and safe alternative to chemical pesticides. Entomopathogenic fungi effectively infect and eliminate insects, thereby contributing to the control of insect populations through the induction of lethal infections known as epizootics. Isolating EPF from the soil is an effective method as they naturally inhabit soil ecosystems. The north-eastern region of India possesses a forest cover exceeding 80%, with Manipur alone accounting for nearly 75% of forest cover in its total geographical area. This abundant forest cover, along with undisturbed land, contributes to the region’s wealth of micro flora and fauna, including a thriving population of entomopathogenic fungi. However, the potential of these fungi in pest population management remains largely unexplored. Therefore, this study was conducted to investigate the diversity of these promising entomopathogenic fungi. Methods: In this study, we isolated fungi from the soils of ten districts of Manipur and identified several isolates with entomopathogenic properties. Soil bating using Galleria mellonella larvae was employed for the isolation of entomopathogenic fungi. Result: A total of 73 fungal isolates were obtained from 100 soil samples, out of which 54 were identified as entomopathogenic fungi. The genus Aspergillus constituted the most commonly isolated entomopathogenic fungi, followed by isolates of Beauveria, Clonostachys, Talaromyces, Trichoderma, Fusarium, Aspergillus, Candida and Meyerozyma genera. Diversity studies revealed variations in the types and proportions of fungi among different regions of Manipur. Pathogenicity tests confirmed the virulence of the isolated entomopathogenic fungi, with 14 isolates of Beauveria bassiana and two isolates of Talaromyces purpureogenus causing 100% mortality of the test insects. The isolated fungi exhibited excellent performance in insect control and could be further mass-produced for effective pest management.

show abstract

“…An assay was performed to estimate natural occurrence (%) of EPNs in soil of the study site using the insect bait method according to Bedding and Akhurst (1975). Last-instar larvae of greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae) were used as baits, reared using artificial diet containing honey (900 g), bee wax (200 g), glycerin (900 g), rice flour (1 300 g), and yeast (400 g) under temperature of 25±1 °C, relative humidity (RH) of 65±5%, and lighting period of 16 h (Birah et al, 2008). Accordingly, each soil sample was mixed well and then, 250 g of the soil, as sub-sample, was transferred to a plastic container (10×10×5 cm).…”

Section: Evaluation Of Epns ҆ Populationmentioning

confidence: 99%

Populations of entomopathogenic nematodes in agricultural lands

VAFA¹,

SAEEDIZADEH²

2022

JCEA

View full text Add to dashboard Cite

Entomopathogenic nematodes (EPNs) are known agents in pest biological control. Environmental factors can influence on populations of soil-inhabitant nematodes like EPNs in agricultural lands. In the current study, after selecting a suitable study site (33.20-33.42 N and 48-48.55 E), and soil sampling (250 samples) in May to July 2019, infectious juveniles of EPNs were extracted from soil samples according to the bait method. According to occurrence of EPNs-infected Galleria mellonella larvae, natural occurrence (%) of EPNs was estimated, while considering soil characteristics (salinity and acidity), region (altitude) and type of crop. Also, population incidence (%) of EPNs in EPNs-positive soil samples was estimated, according to G. mellonella larvae mortality by re-baiting. As results, seven soil samples were found to be EPNspositive per total samples. Natural occurrence (%) of EPNs, Steinernematidae and Heterorhabditidae was equal to 2.8, 2, and 0.8%, respectively. This was variable under ranges of area altitude, soil salinity and acidity, and crop type, so that the highest to lowest occurrence (%) of EPNs was observed in forage crops (4%), vegetables (4%), industrial crops (4%), fruit trees (2%), and cereals (1.33%), respectively. There was a significant difference (P<0.05) in incidence of EPNs ҆ population (%) in EPNs-positive samples. The lowest-highest incidence of EPNs ҆ population was obtained as 15.52-33.4% in 7 EPNspositive samples. As a conclusion, type of crop, altitude and soil acidity and salinity play a role in the level of occurrence and incidence of EPNs populations. These may have affected the biological and ecological properties of the nematodes populations.

show abstract

Modified semi-synthetic diet for mass rearing of wax moth Galleria mellonella L.

Cited by 3 publications

References 0 publications

Effect of the Biostimulants of Microbiological Origin on the Entomopathogenic and Plant Parasitic Nematodes from Miscanthus × Giganteus Plantations

Effect of the Biostimulants of Microbiological Origin on the Entomopathogenic and Plant Parasitic Nematodes from Miscanthus × Giganteus Plantations

Isolation and Identification of Entomopathogenic Fungi from Soils of Manipur (N-E India)

Populations of entomopathogenic nematodes in agricultural lands

Contact Info

Product

Resources

About