Modifying last layer in polyelectrolyte multilayer coatings for capillary electrophoresis of proteins

Roca, Sébastien; Leclercq, Laurent; Gonzalez, Philippe; Dhellemmes, Laura; Boiteau, Laurent; Rydzek, Gaulthier; Cottet, Hervé

doi:10.1016/j.chroma.2023.463837

Cited by 9 publications

(6 citation statements)

References 53 publications

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“…The thickness measurements, shown in Table , are in accordance with results found with a quartz microbalance (QCM) for the same coating . Cleaning the initial glass surface with piranha solution or SDS, isopropanol, and water was essential to remove impurities.…”

Section: Resultssupporting

confidence: 82%

Dual Contributions of Analyte Adsorption and Electroosmotic Inhomogeneity to Separation Efficiency in Capillary Electrophoresis of Proteins

Dhellemmes,

Leclercq,

Lichtenauer

et al. 2024

Anal. Chem.

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Improving separation efficiency in capillary electrophoresis (CE) requires systematic study of the influence of the electric field (or solute linear velocity) on plate height for a better understanding of the critical parameters controlling peak broadening. Even for poly(diallyldimethylammonium chloride) (PDADMAC)/poly(sodium styrenesulfonate) (PSS) successive multiple ionic-polymer layer (SMIL) coatings, which lead to efficient and reproducible separations of proteins, plate height increases with migration velocity, limiting the use of high electric fields in CE. Solute adsorption onto the capillary wall was generally considered as the main source of peak dispersion, explaining this plate height increase. However, experiments done with Taylor dispersion analysis and CE in the same conditions indicate that other phenomena may come into play. Protein adsorption with slow kinetics and few adsorption sites was established as a source of peak broadening for specific proteins. Surface charge inhomogeneity was also identified as a contribution to plate height due to local electroosmotic fluctuations. A model was proposed and applied to partial PDADMAC/poly(ethylene oxide) capillary coatings as well as PDADMAC/PSS SMIL coatings. Atomic force microscopy with topography and recognition imaging enabled the determination of roughness and charge distribution of the PDADMAC/PSS SMIL surface.

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Section: Resultssupporting

confidence: 82%

Dual Contributions of Analyte Adsorption and Electroosmotic Inhomogeneity to Separation Efficiency in Capillary Electrophoresis of Proteins

Dhellemmes,

Leclercq,

Lichtenauer

et al. 2024

Anal. Chem.

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“…Therefore, careful consideration of the capillary surface is crucial in achieving reliable and reproducible results in CE analysis. Capillary coating is a commonly used strategy in CE research as it can offer highly stable EOF and prevent analyte adsorption onto the capillary inner wall, resulting in improved separation efficiency and peak quality. − …”

Section: Methods Development and Technology Advancesmentioning

confidence: 99%

Capillary Electrophoresis: A Three-Year Literature Review

Li,

Miao,

Tan

et al. 2024

Anal. Chem.

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“…Permanently coated capillaries are commercially available, but the id is usually limited to 50 μm or greater and the price is usually high. Successive multiple ionic polymer layers coating, which combines alternating polycationic and polyanionic polymer layers boosting the durability of the coating, was successfully used in several recent publications [122][123][124][125][126], however, more studies are needed to investigate the performance of successive multiple ionic polymer layers for CE-MS top-down proteomics. An interesting approach for on-line preconcentration of proteins through switchable protein adsorption/desorption properties of mixed polymer brushes coatings was applied for on-line preconcentration of lysozyme in hen egg white [127,128], bovine serum albumin [129] and pepsin [130].…”

Section: Coatingmentioning

confidence: 99%

Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Maráková

Opetová

Tomašovský

2023

J of Separation Science

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Capillary electrophoresis is recognized as a valued separation technique for its high separation efficiency, low sample consumption, good economic and ecological aspects, reproducibility, and complementarity to traditional liquid chromatography techniques. Capillary electrophoresis experiments are generally performed utilizing optical detection, such as ultraviolet or fluorescence detectors. However, in order to provide structural information, capillary electrophoresis hyphenated to highly sensitive and selective mass spectrometry has been developed to overcome the limitations of optical detections. Capillary electrophoresis‐mass spectrometry is increasingly popular in protein analysis, including biopharmaceutical and biomedical research. It is frequently applied for the determination of physicochemical and biochemical parameters of proteins, offers excellent performance for in‐depth characterizations of biopharmaceuticals at various levels of analysis, and has been also already proven as a promising tool in biomarker discovery. In this review, we focus on the possibilities and limitations of capillary electrophoresis‐mass spectrometry for protein analysis at their intact level. Various capillary electrophoresis modes and capillary electrophoresis‐mass spectrometry interfaces, as well as approaches to prevent protein adsorption and to enhance sample loading capacity, are discussed and the recent (2018–March 2023) developments and applications in the field of biopharmaceutical and biomedical analysis are summarized.

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Modifying last layer in polyelectrolyte multilayer coatings for capillary electrophoresis of proteins

Cited by 9 publications

References 53 publications

Dual Contributions of Analyte Adsorption and Electroosmotic Inhomogeneity to Separation Efficiency in Capillary Electrophoresis of Proteins

Dual Contributions of Analyte Adsorption and Electroosmotic Inhomogeneity to Separation Efficiency in Capillary Electrophoresis of Proteins

Capillary Electrophoresis: A Three-Year Literature Review

Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

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