Modifying the host range properties of retroviral vectors

Russell, Stephen J.; Cosset, F.-L.

doi:10.1002/(sici)1521-2254(199909/10)1:5<300::aid-jgm59>3.0.co;2-t

Cited by 80 publications

(44 citation statements)

References 94 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Substantial progress has been achieved towards this goal: (1) human complement-resistant retroviral vectors can now be generated thanks to the development of human cell-derived packaging cell lines 1,2 and to the use of specific retroviral envelope glycoproteins; 1,3 (2) methods that optimize the production and quality of retroviral vectors have been developed; 4,5 (3) several strategies aimed at targeting gene delivery and/or expression have been described; [6][7][8] and (4) new vectors that allow gene delivery into nonproliferating cells have now emerged. 9,10 Retroviral vectors derived from murine leukemia viruses (MLVs) and from other type C and D mammalian retroviruses have proved to be extremely useful and adaptable in transducing target cells cultivated in vitro or ex vivo.…”

Section: Introductionmentioning

confidence: 99%

Characterization of novel safe lentiviral vectors derived from simian immunodeficiency virus (SIVmac251) that efficiently transduce mature human dendritic cells

et al. 2000

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Characterization of novel safe lentiviral vectors derived from simian immunodeficiency virus (SIVmac251) that efficiently transduce mature human dendritic cells

et al. 2000

View full text Add to dashboard Cite

“…These applications will benefit from advances in the fields of transcriptional targeting 34 and targeting vectors. 35,36 In summary, hapten-directed targeting of agents to sites of receptor transgene expression takes advantage of the high selectivity of antibody-antigen interactions. scFv's can be efficiently expressed on cells by employing an extracellular domain to reduce cleavage and a TM domain with an intact cytoplasmic tail to anchor the receptors on the cell surface.…”

Section: Discussionmentioning

confidence: 99%

Hapten-directed targeting to single-chain antibody receptors

et al. 2004

View full text Add to dashboard Cite

Artificial recombinant receptors may be useful for selectively targeting imaging and therapeutic agents to sites of gene expression. To evaluate this approach, we developed transgenes to express highly on cells a single-chain antibody (scFv) against the hapten 4-ethoxymethylene-2-phenyl-2-oxazoline-5-one (phOx). A phOx enzyme conjugate was created by covalently attaching phOx molecules to polyethylene glycol (PEG)-modified b-glucuronidase. Cells expressing phOx scFv but not control scFv receptors were selectively killed after exposure to -glucuronidase derivatized with phOx and PEG (phOx-bG-PEG) and a glucuronide prodrug (phydroxy aniline mustard b-D-glucuronide, HAMG) of p-hydroxyaniline mustard. Targeted activation of HAMG produced bystander killing of receptor-negative cells in mixed populations containing as few as 10% phOx-receptor-positive cells. Functional phOx scFv receptors were stably expressed on B16-F1 melanoma tumors in vivo. Treatment of mice bearing established phOx-receptorpositive tumors with phOx-bG-PEG and HAMG significantly (Pp.0005) suppressed tumor growth as compared with treatment with bG-PEG and HAMG or prodrug alone. phOx was unstable in the serum, suggesting alternative haptens may be more suitable for in vivo applications. Our results show that therapeutic agents can be targeted to artificial hapten receptors in vitro and in vivo. The expression of artificial receptors on target cells may allow preferential delivery of therapeutic or imaging molecules to sites of transgene expression.

show abstract

“…To minimize the disruptive effects of large polypeptide insertions on envelope functions such as membrane fusion, shorter receptor-binding motifs have been rationally inserted into permissive sites within envelope proteins. However, with few exceptions (Hatziioannou et al, 1999;Patterson et al, 1999;Russell and Cosset, 1999), an incomplete understanding of the complicated structurefunction relationships of viral envelopes still limits our ability to rationally engineer targeted and efficient envelope proteins. For example, the binding of the envelope protein to a cell surface receptor often triggers viral fusion with the cell membrane, and the complex fusion domains can be widely distributed throughout an envelope protein (Kayman et al, 1999;Li et al, 1993;Martinez and Wertz, 2005;Schlehuber and Rose, 2004).…”

Section: Retroviral Vectorsmentioning

confidence: 99%

Library selection and directed evolution approaches to engineering targeted viral vectors

Jang

Lim

Schaffer

2007

Biotech & Bioengineering

View full text Add to dashboard Cite

Gene therapy, to delivery of genetic material to a patient for therapeutic benefit, has significant promise for translating basic knowledge of disease mechanism into biomedical treatments. The clinical development of the field has been slowed, however, by the need for improvements in the properties and capabilities of gene delivery vehicles. Vehicles based on viruses offer the potential for efficient gene delivery, but because viruses did not evolve to serve human therapeutic needs, many of their properties require significant improvement, including their safety, efficiency, and capacity for targeted gene delivery. Since viruses are highly complex biological entities, engineering such properties at the molecular level can be challenging. However, there has been significant progress in developing approaches that mimic the mechanisms by which viruses arose in the first place. In particular, library-based selection, the generation of one diverse genetic library and selection for new properties, and directed evolution, based on the multiple rounds of library generation and selection for iterative improvement of function, have strong potential in engineering novel properties into these complex biomolecular assemblies. This review will discuss progress in the application of peptide display, library selection, and directed evolution technologies toward engineering vectors based on retrovirus, adeno-associated virus, and adenovirus that are capable of targeted delivery to specific cell types. In addition to creating biomedically useful products, these approaches have future potential to yield novel insights into viral structure-function relationships.

show abstract

Modifying the host range properties of retroviral vectors

Cited by 80 publications

References 94 publications

Characterization of novel safe lentiviral vectors derived from simian immunodeficiency virus (SIVmac251) that efficiently transduce mature human dendritic cells

Characterization of novel safe lentiviral vectors derived from simian immunodeficiency virus (SIVmac251) that efficiently transduce mature human dendritic cells

Hapten-directed targeting to single-chain antibody receptors

Library selection and directed evolution approaches to engineering targeted viral vectors

Contact Info

Product

Resources

About