Modular bioink for 3D printing of biocompatible hydrogels: sol–gel polymerization of hybrid peptides and polymers

Echalier, Cécile; Levato, Riccardo; Mateos‐Timoneda, Miguel A.; Castaño, Óscar; Dejean, Stéphane; Garric, Xavier; Pinese, Coline; Noël, Danièle; Engel, Elisabeth; Martinez, Jean; Mehdi, Ahmad; Subra, Gilles

doi:10.1039/c6ra28540f

Cited by 43 publications

(41 citation statements)

References 30 publications

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“…After this point, viscosity increased quickly. We determined that the ‘ideal’ printing window corresponded to a hydrogel viscosity comprised between 2500 and 4500 mPa.s, allowing the 3D printing of this hydrogel with a good retention of the shape, enabling extrusion and avoiding spreading . Besides, the slope of the curve of viscosity as a function of time was calculated between the lowest and the highest limit of this interval; the smaller the value, the bigger amount of time usable for printing.…”

Section: Resultsmentioning

confidence: 99%

“…They were obtained by click chemistry (e. g. Michael addition, Schiff‐base reaction, aldehyde‐hydrazide ligation), photo‐polymerization and enzymatic reactions . In that context we have developed the sol‐gel inorganic polymerization as a new method for 3D printing . Sol‐gel is a well‐known process allowing the preparation of oxides or hybrid materials in soft conditions.…”

Section: Introductionmentioning

confidence: 99%

“…We have also demonstrated that short peptide sequences inspired from collagen could also be used to obtain hydrogels in which cells can be embedded . However, only few examples of sol‐gel process hydrogels applied to 3D‐printing have been published, most of the time incompatible with cell encapsulation due to reaction conditions (acidic conditions or use of toxic catalysts) …”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9] In that context we have developed the solgel inorganic polymerization as a new method for 3D printing. [10] Sol-gel is a well-known process [11][12][13][14] allowing the preparation of oxides or hybrid materials in soft conditions. Hydrolysis and condensation of alkoxysilanes may proceed in water, in a chemoselective way, and can be considered as a bioorthogonal reaction.…”

Section: Introductionmentioning

confidence: 99%

“…[16] However, only few examples of sol-gel process hydrogels applied to 3D-printing have been published, most of the time incompatible with cell encapsulation due to reaction conditions (acidic conditions or use of toxic catalysts). [10,17,18] The main complexity of using bioink based on chemical hydrogel is to control its viscosity, as it increases simultaneously with the reticulation. For this reason, a suitable 'printing window' has to be determined, i. e. a period when the viscosity is sufficient to print with retention of the shape and without flowing, For extrusion printing, this window should be comprised between 30 and 6.10 7 mPa.s, depending of the nozzle's size, shape and the type of extruder (e. g. endless screw, air compressor).…”

Section: Introductionmentioning

confidence: 99%

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Biocompatible Glycine‐Assisted Catalysis of the Sol‐Gel Process: Development of Cell‐Embedded Hydrogels

et al. 2019

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The sol‐gel process can be used for hydrogel cross‐linking, thus opening an attractive route for the design of biocompatible hydrogels under soft conditions. The sol‐gel process can be catalysed at basic or acidic pH values, under neutral conditions with the addition of a nucleophile. Therefore, working around pH 7 unlocks the possibility of direct cell embedment and the preparation of bioinks. We aimed to propose a generic method for sol‐gel 3D bioprinting, and first screened different nucleophilic catalysts using bis‐silylated polyethylene glycol (PEG) as a model hydrogel. A synergistic effect of glycine and NaF, used in low concentrations to avoid any toxicity, was observed. Biocompatibility of the approach was demonstrated by embedding primary mouse mesenchymal stem cells. The measure of viscosity as a function of time showed the impact of reaction parameters, such as temperature, complexity of the medium, pH and cell addition, on the kinetics of the sol‐gel process, and allowed prediction of the gelation time.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biocompatible Glycine‐Assisted Catalysis of the Sol‐Gel Process: Development of Cell‐Embedded Hydrogels

et al. 2019

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Advances in Extrusion 3D Bioprinting: A Focus on Multicomponent Hydrogel‐Based Bioinks

Cui

Hartanto

et al. 2020

Adv Healthcare Materials

257

214

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Abstract3D bioprinting involves the combination of 3D printing technologies with cells, growth factors and biomaterials, and has been considered as one of the most advanced tools for tissue engineering and regenerative medicine (TERM). However, despite multiple breakthroughs, it is evident that numerous challenges need to be overcome before 3D bioprinting will eventually become a clinical solution for a variety of TERM applications. To produce a 3D structure that is biologically functional, cell‐laden bioinks must be optimized to meet certain key characteristics including rheological properties, physico‐mechanical properties, and biofunctionality; a difficult task for a single component bioink especially for extrusion based bioprinting. As such, more recent research has been centred on multicomponent bioinks consisting of a combination of two or more biomaterials to improve printability, shape fidelity and biofunctionality. In this article, multicomponent hydrogel‐based bioink systems are systemically reviewed based on the inherent nature of the bioink (natural or synthetic hydrogels), including the most current examples demonstrating properties and advances in application of multicomponent bioinks, specifically for extrusion based 3D bioprinting. This review article will assist researchers in the field in identifying the most suitable bioink based on their requirements, as well as pinpointing current unmet challenges in the field.

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Peptide Hydrogels as Immunomaterials and Their Use in Cancer Immunotherapy Delivery

Falcone,

Ermis,

Tamay

et al. 2023

Adv Healthcare Materials

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Peptide‐based hydrogel biomaterials have emerged as an excellent strategy for immune system modulation. Peptide‐based hydrogels are supramolecular materials that self‐assemble into various nanostructures through various interactive forces (i.e., hydrogen bonding and hydrophobic interactions) and respond to microenvironmental stimuli (i.e., pH, temperature). While they have been reported in numerous biomedical applications, they have recently been deemed promising candidates to improve the efficacy of cancer immunotherapies and treatments. Immunotherapies seek to harness the body's immune system to preemptively protect against and treat various diseases, such as cancer. However, their low efficacy rates result in limited patient responses to treatment. Here, the immunomaterial's potential to improve these efficacy rates by either functioning as immune stimulators through direct immune system interactions and/or delivering a range of immune agents is highlighted. The chemical and physical properties of these peptide‐based materials that lead to immuno modulation and how one may design a system to achieve desired immune responses in a controllable manner are discussed. Works in the literature that reports peptide hydrogels as adjuvant systems and for the delivery of immunotherapies are highlighted. Finally, the future trends and possible developments based on peptide hydrogels for cancer immunotherapy applications are discussed.

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Modular bioink for 3D printing of biocompatible hydrogels: sol–gel polymerization of hybrid peptides and polymers

Abstract: Inorganic polymerization as a cross-linking method for 3D printing of PEG–peptide hydrogels.

Cited by 43 publications

References 30 publications

Biocompatible Glycine‐Assisted Catalysis of the Sol‐Gel Process: Development of Cell‐Embedded Hydrogels

Biocompatible Glycine‐Assisted Catalysis of the Sol‐Gel Process: Development of Cell‐Embedded Hydrogels

Advances in Extrusion 3D Bioprinting: A Focus on Multicomponent Hydrogel‐Based Bioinks

Peptide Hydrogels as Immunomaterials and Their Use in Cancer Immunotherapy Delivery

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