Modular design of a hybrid hydrogel for protease-triggered enhancement of drug delivery to regulate TNF-α production by pro-inflammatory macrophages

Nguyen, Dang Tri; Soeranaya, Bob Hartadhi Tji; Truong, Thi Hong Anh; Dang, Tram T.

doi:10.1016/j.actbio.2020.09.026

Cited by 14 publications

(7 citation statements)

References 77 publications

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“…Many strategies are developed for wound healing. In the early stage of recovery (several hours to days), it is an effective approach to introduce the reducing agents such as the derivatives of dopamine, , arginine, phenylborate esters, and tetraaniline (TA) to scavenge ROS and polarizing of macrophages toward M2 phenotype by bioactive materials or immunomodulatory drugs and genes . In the mid and late stages of recovery (several days to months), more efforts should be devoted to promote proliferation by improving cell affinity and inhibition of apoptosis, , vascularization by regulation of genes and microenvironments, and collagen deposition by inhibition of metalloproteinases (MMPs).…”

Section: Introductionmentioning

confidence: 99%

Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing

Jin

Shang

Zhan

et al. 2020

ACS Appl. Mater. Interfaces

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Injectable hydrogels with the capability to cast a hypoxic microenvironment is of great potentialities to develop novel therapies for tissue regeneration. However, the relative research still remains at the conceptual phase. Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxiainduced hydrogels. To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking. Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol−ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment. The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions. This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.

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Section: Introductionmentioning

confidence: 99%

Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing

Jin

Shang

Zhan

et al. 2020

ACS Appl. Mater. Interfaces

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“…Additionally, it was discovered that knocking down lncZFY-AS1 reduced inflammatory factors TNF-α, IL-1β, and IL-6 in the periodontitis model. It is worth noting that these inflammatory factors are mainly released by macrophages under inflammatory stimulation [ 34 ]. Therefore, it was speculated that preventing macrophage infiltration is a momentous measure for lncZFY-AS1 to repress periodontitis inflammation.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA ZFY-AS1 represses periodontitis tissue inflammation and oxidative damage via modulating microRNA-129-5p/DEAD-Box helicase 3 X-linked axis

Cheng

Fan

Cheng³

et al. 2022

Bioengineered

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A large number of studies have manifested long non-coding RNA (lncRNA) is involved in the modulation of the development of periodontitis, but the specific mechanism has not been fully elucidated. The purpose of this study was to explore the biological function and latent molecular mechanism of lncZFY-AS1 in periodontitis. The results clarified lncZFY-AS1 and DEAD-Box Helicase 3 X-Linked (DDX3X) were up-regulated, but microRNA (miR)-129-5p was down-regulated in periodontitis. Knockdown of lncZFY-AS2 or overexpression of miR-129-5p decreased macrophage infiltration and periodontal membrane cell apoptosis, increased cell viability, repressed inflammatory factors and nuclear factor kappa B activation, reduced oxidative stress, but promoted nuclear factor-E2-related factor 2/heme oxygenase 1 expression. LncZFY-AS1 elevation further aggravated periodontitis inflammation, oxidative stress, and apoptosis. LncZFY competitively adsorbed miR-129-5p to mediate DDX3X expression. Knockdown lncZFY’s improvement effect on periodontitis was reversed by depressive miR-129-5p or enhancive DDX3X. In conclusion, these data suggest lncZFY-AS1 promotes inflammatory injury and oxidative stress in periodontitis by competitively binding to miR-129-5p and mediating DDX3X expression. LncZFY-AS1/miR-129-5p/DDX3X may serve as a novel molecular target for treatment of periodontitis in the future.

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“…Hydrogels loading anti-inflammatory drugs have also been designed to modulate macrophages. For example, protease-cleavable hydrogel has been designed to locally deliver PLGA NPs loaded with ibuprofen to inflamed tissues where elevated protease activity is found [ 135 ]. Delivered ibuprofen inhibited TNFα expression in RAW 264.7 murine macrophages ( Figure 7 ).…”

Section: Nanocomposite Hydrogels To Modulate Macrophagesmentioning

confidence: 99%

Nanoparticles to Target and Treat Macrophages: The Ockham’s Concept?

2021

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Nanoparticles are nanomaterials with three external nanoscale dimensions and an average size ranging from 1 to 1000 nm. Nanoparticles have gained notoriety in technological advances due to their tunable physical, chemical, and biological characteristics. However, the administration of functionalized nanoparticles to living beings is still challenging due to the rapid detection and blood and tissue clearance by the mononuclear phagocytic system. The major exponent of this system is the macrophage. Regardless the nanomaterial composition, macrophages can detect and incorporate foreign bodies by phagocytosis. Therefore, the simplest explanation is that any injected nanoparticle will be probably taken up by macrophages. This explains, in part, the natural accumulation of most nanoparticles in the spleen, lymph nodes, and liver (the main organs of the mononuclear phagocytic system). For this reason, recent investigations are devoted to design nanoparticles for specific macrophage targeting in diseased tissues. The aim of this review is to describe current strategies for the design of nanoparticles to target macrophages and to modulate their immunological function involved in different diseases with special emphasis on chronic inflammation, tissue regeneration, and cancer.

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Modular design of a hybrid hydrogel for protease-triggered enhancement of drug delivery to regulate TNF-α production by pro-inflammatory macrophages

Cited by 14 publications

References 77 publications

Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing

Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing

Long non-coding RNA ZFY-AS1 represses periodontitis tissue inflammation and oxidative damage via modulating microRNA-129-5p/DEAD-Box helicase 3 X-linked axis

Nanoparticles to Target and Treat Macrophages: The Ockham’s Concept?

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