Modular Design of Picroside-II Biosynthesis Deciphered through NGS Transcriptomes and Metabolic Intermediates Analysis in Naturally Variant Chemotypes of a Medicinal Herb, Picrorhiza kurroa

Kumar, Varun; Bansal, Ankush; Chauhan, Rajinder Singh

doi:10.3389/fpls.2017.00564

Cited by 24 publications

(7 citation statements)

References 67 publications

(87 reference statements)

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“…It is currently known that the rootstock can alter the gene expression in the scion, especially in the presence of stress, disease or limiting factors. Several transcriptome changes are related to the phenylpropanoid pathway genes, like those responsible for stilbene and flavonoid biosynthesis [ 13 , 20 – 25 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and biochemical investigations support the role of rootstock-scion interaction in grapevine berry quality

Zombardo

Crosatti²,

Bagnaresi³

et al. 2020

BMC Genomics

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Background: In viticulture, rootstock genotype plays a critical role to improve scion physiology, berry quality and to adapt grapevine (Vitis vinifera L.) to different environmental conditions. This study aimed at investigating the effect of two different rootstocks (1103 Paulsen-P-and Mgt 101-14-M) in comparison with not grafted plants-NGC-on transcriptome (RNA-seq and small RNA-seq) and chemical composition of berry skin in Pinot noir, and exploring the influence of rootstock-scion interaction on grape quality. Berry samples, collected at veraison and maturity, were investigated at transcriptional and biochemical levels to depict the impact of rootstock on berry maturation. Results: RNA-and miRNA-seq analyses highlighted that, at veraison, the transcriptomes of the berry skin are extremely similar, while variations associated with the different rootstocks become evident at maturity, suggesting a greater diversification at transcriptional level towards the end of the ripening process. In the experimental design, resembling standard agronomic growth conditions, the vines grafted on the two different rootstocks do not show a high degree of diversity. In general, the few genes differentially expressed at veraison were linked to photosynthesis, putatively because of a ripening delay in not grafted vines, while at maturity the differentially expressed genes were mainly involved in the synthesis and transport of phenylpropanoids (e.g. flavonoids), cell wall loosening, and stress response. These results were supported by some differences in berry phenolic composition detected between grafted and not grafted plants, in particular in resveratrol derivatives accumulation. Conclusions: Transcriptomic and biochemical data demonstrate a stronger impact of 1103 Paulsen rootstock than Mgt 101-14 or not grafted plants on ripening processes related to the secondary metabolite accumulations in berry skin tissue. Interestingly, the MYB14 gene, involved in the feedback regulation of resveratrol biosynthesis was upregulated in 1103 Paulsen thus supporting a putative greater accumulation of stilbenes in mature berries.

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Section: Introductionmentioning

confidence: 99%

Transcriptomic and biochemical investigations support the role of rootstock-scion interaction in grapevine berry quality

Zombardo

Crosatti²,

Bagnaresi³

et al. 2020

BMC Genomics

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“…Active fractions and pure compounds from P. Kurroa have been reported to exhibit anti-asthma, antiin ammation, hepatoprotection, immunostimulation, and free radical scavenging properties (Table S1). These pharmacological properties are mostly attributed to the presence of a group of monoterpenoid bicyclic iridoid glycosides of catalposide class, namely, P-I, P-II, 6-feruloyl catalpol, and pikuroside [10,15]. Among these compounds, P-II and pikuroside are found mainly in roots and rhizomes and structurally both of these compounds are constituted of two moieties, a catalpol and a vanillic acid moiety bonded in a ester linkage at C-6 of the backbone catalpol moiety.…”

Section: Discussionmentioning

confidence: 99%

“…Picroside-II (P-II, amphicoside) is a major iridoid found in stolons and roots of P. kurroa [10]. P-II has a vanilloyl moiety acylated to C-6 of a catalpol (CAT) moiety and is also commercially referred to as 6vanillyl catalpol (https://www.sigmaaldrich.com/DE/en/search/amphicoside).…”

Section: Introductionmentioning

confidence: 99%

Capturing Acyltransferase(s) Transforming Final Step in the Biosynthesis of a Major Iridoid Glycoside, (Picroside-II) in a Himalayan Medicinal Herb, Picrorhiza kurroa

Kharb

Sharma

et al. 2021

Preprint

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BackgroundPicrorhiza kurroa has been reported as an age-old ayurvedic hepatoprotection to treat hepatic disorders due to the presence of iridoids such as picroside-II (P-II), picroside-I, and kutkoside. The acylation of catalpol and vanilloyl coenzyme A by acyltransferases (ATs) is critical step in P-II biosynthesis. Since accumulation of P-II occurs only in roots, rhizomes and stolons, uprooting of this critically endangered herb has been the only source of this compound. Recently, we reported that P-II acylation likely happen in roots, while stolons serve as the vital P-II storage compartment. Therefore, developing an alternate engineered platform for P-II biosynthesis require identification of P-II specific AT/s.Methods and results In that direction, egg-NOG function annotated 815 ATs from de novo RNA sequencing of tissue culture based ‘shoots-only’ system and nursery grown shoots, roots, and stolons varying in P-II content, were cross-compared in silico to arrive at ATs sequences unique and/or common to stolons and roots. Verification for organ and accession-wise upregulation in gene expression of these ATs by qPCR has shortlisted six putative ‘P-II-forming’ ATs. Further, six-frame translation, ab initio protein structure modelling and protein-ligand molecular docking of these ATs signified one MBOAT domain containing AT with preferential binding to the vanillic acid CoA thiol ester as well as with P-II., implying that this could be potential AT decorating final structure of P-II. ConclusionOrgan-wise comparative transcriptome mining coupled with reverse transcription real time qPCR and protein-ligand docking led to the identification of an acyltransferases, contributing to the final structure of P-II.

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“…Morphologically, plants are herbaceous with an elongated rhizome (rootstock) and aerial portion consists of basal leaves and terminal inflorescence (flowering spike) [ 5 ]. P. kurroa propagates vegetatively as young buds on rhizomes, which subsequently develops as a new rhizome with autonomous root and shoot sections [ 6 ]. In addition, rhizome survives underground during snow cover whereas, shoot emerges rapidly during onset of suitable growth conditions.…”

Section: Introductionmentioning

confidence: 99%

In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry

et al. 2021

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Background Picrorhiza kurroa Royle ex Benth. being a rich source of phytochemicals, is a promising high altitude medicinal herb of Himalaya. The medicinal potential is attributed to picrosides i.e. iridoid glycosides, which synthesized in organ-specific manner through highly complex pathways. Here, we present a large-scale proteome reference map of P. kurroa, consisting of four morphologically differentiated organs and two developmental stages. Results We were able to identify 5186 protein accessions (FDR < 1%) providing a deep coverage of protein abundance array, spanning around six orders of magnitude. Most of the identified proteins are associated with metabolic processes, response to abiotic stimuli and cellular processes. Organ specific sub-proteomes highlights organ specialized functions that would offer insights to explore tissue profile for specific protein classes. With reference to P. kurroa development, vegetative phase is enriched with growth related processes, however generative phase harvests more energy in secondary metabolic pathways. Furthermore, stress-responsive proteins, RNA binding proteins (RBPs) and post-translational modifications (PTMs), particularly phosphorylation and ADP-ribosylation play an important role in P. kurroa adaptation to alpine environment. The proteins involved in the synthesis of secondary metabolites are well represented in P. kurroa proteome. The phytochemical analysis revealed that marker compounds were highly accumulated in rhizome and overall, during the late stage of development. Conclusions This report represents first extensive proteomic description of organ and developmental dissected P. kurroa, providing a platform for future studies related to stress tolerance and medical applications.

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Modular Design of Picroside-II Biosynthesis Deciphered through NGS Transcriptomes and Metabolic Intermediates Analysis in Naturally Variant Chemotypes of a Medicinal Herb, Picrorhiza kurroa

Cited by 24 publications

References 67 publications

Transcriptomic and biochemical investigations support the role of rootstock-scion interaction in grapevine berry quality

Transcriptomic and biochemical investigations support the role of rootstock-scion interaction in grapevine berry quality

Capturing Acyltransferase(s) Transforming Final Step in the Biosynthesis of a Major Iridoid Glycoside, (Picroside-II) in a Himalayan Medicinal Herb, Picrorhiza kurroa

In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry

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