2012
DOI: 10.1016/j.molcatb.2011.12.010
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Modular microfluidic reactor and inline filtration system for the biocatalytic synthesis of chiral metabolites

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Cited by 42 publications
(37 citation statements)
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“…This simple colored food dye perfusion experiment demonstrated that the perfusion functionality of the cellular acetate membrane was retained after 3D printing; demonstrating the feasibility of using the FDM-based 3D printing and embedding method to fabricate porous membrane-based microfluidic devices. The first 3D printed perfusion fluidic device is applicable to continuous microcarrier-based cell culture or biocatalytic synthesis applications similar to the one described by Abeille et al 29 and O'Sullivan et al, 30 respectively, but without the need for any postdevice assembly and finishing.…”
Section: Resultsmentioning
confidence: 99%
“…This simple colored food dye perfusion experiment demonstrated that the perfusion functionality of the cellular acetate membrane was retained after 3D printing; demonstrating the feasibility of using the FDM-based 3D printing and embedding method to fabricate porous membrane-based microfluidic devices. The first 3D printed perfusion fluidic device is applicable to continuous microcarrier-based cell culture or biocatalytic synthesis applications similar to the one described by Abeille et al 29 and O'Sullivan et al, 30 respectively, but without the need for any postdevice assembly and finishing.…”
Section: Resultsmentioning
confidence: 99%
“…[33][34][35][36] These systems have been used extensively to study upstream bioprocessing steps, [37][38][39][40][41] but the number of reports of microfluidic devices developed to study process optimisation of downstream processing steps, such as purification and separation of biomolecules, is still limited. 27,[42][43][44][45][46][47][48] In downstream bioprocessing, flocculation has attracted renewed interest but suitable analytical tools to comprehensively understand the flocculation mechanism have been absent. The precise control over the microenvironment afforded by microfluidic devices opened up an opportunity to investigate, for the first time, the formation and growth of flocs independently from floc breakage and ageing phases.…”
Section: Discussionmentioning
confidence: 99%
“…The recovery and reuse of enzymes has been achieved using bio-microreactors with immobilized enzymes, a topic discussed later in this paper. Nevertheless, some interesting results were also obtained for continuous flow systems, as in the case of the synthesis of a chiral compound using transketolase [82], where the separation of the enzyme from the other substances was achieved by coupling a tangential flow filtration system to a microfluidic reactor.…”
Section: Bio-microreactors With Free Enzymesmentioning
confidence: 99%
“…A lower yield with respect to a typical chemical synthesis was observed with high substrate concentrations, which was attributed to inhibition and denaturation effects induced by the presence of an excess of substrate molecules in the proximity of the enzyme [82]. To overcome this problem, an interesting strategy incorporating a multi-input microfluidic reactor, capable of substrate feeding at multiple points, was designed and successfully applied to the transketolase-catalyzed synthesis of L-erythrulose [83].…”
Section: Bio-microreactors With Free Enzymesmentioning
confidence: 99%
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