Modulating Cell Behaviors on Chiral Polymer Brush Films with Different Hydrophobic Side Groups

Wang, Xing; Gan, Hui; Zhang, Mingxi; Sun, Taolei

doi:10.1021/la204143g

Cited by 68 publications

(56 citation statements)

References 40 publications

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“…Our recent work has demonstrated that cells exhibited distinct inclination to different chiral stereochemistry during its adhesion on external surfaces, 23,24 which inspired us to make further effort on antimicrobial material or surface catering to the "chiral taste" of microbe cells. A model system by polyborneolacrylate was thus achieved, 25 where L-borneol contented polymer with good biocompatibility was proved to be a promising candidate for antibacterial adhesion.…”

Section: Introductionmentioning

confidence: 99%

Borneol-grafted cellulose for antifungal adhesion and fungal growth inhibition

et al. 2015

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Cellulose fibers modified with antifungal activity have attracted much attention due to their versatile applications, although there are still some problems associated with the conventional protocols, including the toxicity to organisms, unwanted resistance, and gradually increasing environmental pressure. Natural and safe strategy is desired to design new type of antifungal cellulose. Herein, we present a borneol-grafted cellulose (BGC) by covalent tethering Lborneol, a natural product, to cellulose. The attained BGC has been characterized to confirm the chemical and amorphous features using a combination of spectroscopic and analytical techniques. This BGC material was subsequently challenged with Mucor racemosus and Aspergillus niger, and exhibited a remarkable performance in antifungal adhesion and fungal growth inhibition, suggesting the grafted borneol moieties are curial on influencing tactile sense of fungal cells and subsequently selective inadhesion. The BGC has also been evaluated as a non-cytotoxic material, implying its great potential for biomedical and sanitary applications.

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Section: Introductionmentioning

confidence: 99%

Borneol-grafted cellulose for antifungal adhesion and fungal growth inhibition

et al. 2015

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“…After selenocystine immobilization, the water contact angle rises again because of the consumption of the hydrophilic functional groups such as quinone groups. There are no differences between l -Se (68.9 ± 1.4°) and d -Se (68.9 ± 1.1°), indicating that the surface chirality does not influence surface hydrophilicity [27]. However, differences in the water contact angle between the l -Se and d -Se samples are found after protein adsorption (Fig.…”

Section: Resultsmentioning

confidence: 99%

Influence of chirality on catalytic generation of nitric oxide and platelet behavior on selenocystine immobilized TiO2 films

Fan

Xiaxin

Wang

et al. 2016

Colloids and Surfaces B: Biointerfaces

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As nitric oxide (NO) plays vital roles in the cardiovascular system, incorporating this molecule into cardiovascular stents is considered as an effective method. In the present study, selenocystine with different chirality (i.e., l- and d-selenocystine) was used as the catalytic molecule immobilized on TiO2 films for decomposing endogenous NO donor. The influences of surface chirality on NO release and platelet behavior were evaluated. Results show that although the amount of immobilized l-selenocystine on the surface was nearly the same as that of immobilized d-selenocystine, in vitro catalytic NO release tests showed that l-selenocystine immobilized surfaces were more capable of catalyzing the decomposition of S-nitrosoglutathione and thus generating more NO. Accordingly, l-selenocystine immobilized surfaces demonstrated significantly increased inhibiting effects on the platelet adhesion and activation, when compared to d-selenocystine immobilized ones. Measurement of the cGMP concentration of platelets further confirmed that surface chirality played an important role in regulating NO generation and platelet behaviors. Additionally, using bovine serum albumin and fibrinogen as model proteins, the protein adsorption determined with quartz crystal microbalance showed that the l-selenocystine immobilized surface enhanced protein adsorption. In conclusion, surface chirality significantly influences protein adsorption and NO release, which may have significant implications in the design of NO-generating cardiovascular stents.

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“…The adhesion of cells in three dimensional (3D) scaffolds is crucial if cell anoikis is to be avoided, and this is one of the most important issues in fundamental cell biology. 15,16 In order to encourage the adhesion of cells, immobilisation of bioactive molecules such as arginine–glycine–aspartic acid (RGD) peptide, hydroxyapatite or natural polysaccharide is a common strategy to ensure controlled interaction between the cells and the scaffold. 17-19 These methods are based on the formation of integrin-mediated bonds between substrata and the cell membrane.…”

Section: Strengths and Limitationsmentioning

confidence: 99%

Tissue-engineered cartilage constructed by a biotin-conjugated anti-CD44 avidin binding technique for the repairing of cartilage defects in the weight-bearing area of knee joints in pigs

Lin

Zhou

Cao

et al. 2017

Bone & Joint Research

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ObjectivesThe lack of effective treatment for cartilage defects has prompted investigations using tissue engineering techniques for their regeneration and repair. The success of tissue-engineered repair of cartilage may depend on the rapid and efficient adhesion of transplanted cells to a scaffold. Our aim in this study was to repair full-thickness defects in articular cartilage in the weight-bearing area of a porcine model, and to investigate whether the CD44 monoclonal antibody biotin-avidin (CBA) binding technique could provide satisfactory tissue-engineered cartilage.MethodsCartilage defects were created in the load-bearing region of the lateral femoral condyle of mini-type pigs. The defects were repaired with traditional tissue-engineered cartilage, tissue-engineered cartilage constructed with the biotin-avidin (BA) technique, tissue-engineered cartilage constructed with the CBA technique and with autologous cartilage. The biomechanical properties, Western blot assay, histological findings and immunohistochemical staining were explored.ResultsThe CBA group showed similar results to the autologous group in biomechanical properties, Moran’s criteria, histological tests and Wakitani histological scoring.ConclusionsThese results suggest that tissue-engineered cartilage constructed using the CBA technique could be used effectively to repair cartilage defects in the weight-bearing area of joints.Cite this article: H. Lin, J. Zhou, L. Cao, H. R. Wang, J. Dong, Z. R. Chen. Tissue-engineered cartilage constructed by a biotin-conjugated anti-CD44 avidin binding technique for the repairing of cartilage defects in the weight-bearing area of knee joints in pigs. Bone Joint Res 2017;6:–295. DOI: 10.1302/2046-3758.65.BJR-2016-0277.

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Modulating Cell Behaviors on Chiral Polymer Brush Films with Different Hydrophobic Side Groups

Cited by 68 publications

References 40 publications

Borneol-grafted cellulose for antifungal adhesion and fungal growth inhibition

Borneol-grafted cellulose for antifungal adhesion and fungal growth inhibition

Influence of chirality on catalytic generation of nitric oxide and platelet behavior on selenocystine immobilized TiO2 films

Tissue-engineered cartilage constructed by a biotin-conjugated anti-CD44 avidin binding technique for the repairing of cartilage defects in the weight-bearing area of knee joints in pigs

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