Modulating Effect of Lupeol on the Expression Pattern of Apoptotic Markers in 7, 12-Dimethylbenz(a)anthracene Induced Oral Carcinogenesis

Manoharan, Shanmugam; Palanimuthu, Duraisamy; Baskaran, Nagarethinam; Silvan, Simon

doi:10.7314/apjcp.2012.13.11.5753

Cited by 27 publications

(14 citation statements)

References 27 publications

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“…Previous studies from our laboratory reported the chemopreventive potential as well as proapoptotic, anti-inflammatory and anti-angiogenic potential of lupeol in DMBA induced hamster buccal pouch carcinogenesis. [ 69 70 ] Manimaran et al .,[ 71 ] reported that lupeol showed potent anti-cellproliferative, anti-inflammatory and anti-angiogenic potential during DMBA induced hamster buccal pouch carcinogenesis. Rao et al .,[ 72 ] demonstrated the anticancer efficacy of squalene in azoxy methane induced rat colon carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

Modulating effect of Enicostemma littorale on the expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers During 7, 12-Dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis

Manoharan¹,

Rajasekaran²,

Prabhakar³

et al. 2015

Toxicol Int

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Enicostemma littorale leaves are traditionally used for the treatment of several diseases, including inflammation and cancer. This study has taken effort to explore the antitumor initiating potential of E. littorale leaves (ElELet) by analyzing the expression pattern of apoptotic (p53, Bcl-2 and Bcl-2 associated X-protein), cell-proliferative (cyclin D1 and proliferating cell nuclear antigen), angiogenic (vascular endothelial growth factor), invasive (matrix metalloproteinase-2 and 9), and inflammatory (NF-κB and cyclooxygenase-2) markers during 7, 12-dimethylbenz (a) anthracene (DMBA) induced hamster buccal pouch carcinogenesis. Oral tumors were induced in the buccal pouches of hamsters using the potent site and organ specific carcinogen, DMBA. DMBA application 3 times a week for 14 weeks resulted in tumor formation in the buccal pouches. Hundred percent tumor formations with dysregulation in the expression pattern of apoptotic, cell proliferative, inflammatory, angiogenic, and invasive markers were observed in the buccal pouches of hamsters treated with DMBA alone. ElELet at a dose of 250 mg/kg body weight orally to DMBA treated hamsters significantly prevented the tumor formation as well as corrected the abnormalities in the expression pattern of above mentioned molecular markers. ElELet thus modulated the expression pattern of all the above mentioned molecular markers in favor of the suppression of cell proliferation occurring in DMBA induced hamster buccal pouch carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

Modulating effect of Enicostemma littorale on the expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers During 7, 12-Dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis

Manoharan¹,

Rajasekaran²,

Prabhakar³

et al. 2015

Toxicol Int

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“…In the seeds, the terpenoids detected using gas chromatography were β -sitosterol, stigmasterol, lupeol, campesterol, and squalene [14, 15]. Interestingly, lupeol was found to possess anticarcinogenic [16], antinociceptive, and anti-inflammatory properties [17]. No flavonoid like quercetin, myricetin, luteolin, kaempferol, or apigenin was detected in the methanolic extract of P. speciosa seeds using a reversed-phase high performance liquid chromatography [18], but it was noted to be present in the ethanolic extract when screened using a colorimetric assay [10].…”

Section: Chemical Compoundsmentioning

confidence: 99%

Parkia speciosaHassk.: A Potential Phytomedicine

Kamisah

Othman

Qodriyah

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Parkia speciosa Hassk., or stink bean, is a plant indigenous to Southeast Asia. It is consumed either raw or cooked. It has been used in folk medicine to treat diabetes, hypertension, and kidney problems. It contains minerals and vitamins. It displays many beneficial properties. Its extracts from the empty pods and seeds have a high content of total polyphenol, phytosterol, and flavonoids. It demonstrates a good antioxidant activity. Its hypoglycemic effect is reported to be attributable to the presence of β-sitosterol, stigmasterol, and stigmast-4-en-3-one. The cyclic polysulfide compounds exhibit antibacterial activity, while thiazolidine-4-carboxylic acid possesses anticancer property. The pharmacological properties of the plant extract are described in this review. With ongoing research conducted on the plant extracts, Parkia speciosa has a potential to be developed as a phytomedicine.

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“…A large number of oral cancer chemopreventive agents were reported against 7,12-dimethylbenz(a)anthracene (DMBA)-induced oral carcinogenesis (Baskaran et al, 2012;Manoharan et al, 2012a;2012b;Prabhakar et al, 2012).…”

Section: Research Articlementioning

confidence: 99%

Saffron Reduction of 7,12-Dimethylbenz[a]anthracene-induced Hamster Buccal Pouch Carcinogenesis

Manoharan

Wani²,

Vasudevan³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Our aim was to investigate the chemopreventive potential of saffron in DMBA-induced hamster buccal pouch carcinogenesis. Assessment was by monitoring the percentage of tumor bearing hamsters, tumor size as well as the status of detoxification agents, lipid peroxidation and antioxidants. Oral squamous cell carcinomas were induced in the buccal pouch of Syrian golden hamsters by painting them with 0.5% DMBA in liquid paraffin three times a week for 14 weeks. We observed 100% oral tumor formation with severe histopathological abnormalities in all the hamsters treated with DMBA alone, activities of phase I and phase II detoxification enzymes, lipid peroxidation and antioxidants being significantly altered. Though oral administration of saffron completely prevented the formation of tumors, we noticed severe hyperplasia and dysplasia in hamsters treated with DMBA, suggesting that tumors might eventually develop. Oral administration of saffron return detoxification enzymes, lipid peroxidation and antioxidants to normal ranges. The chemopreventive potential of saffron thus is likely due to antioxidant properties and modulating effects on detoxification in favour of the excretion of carcinogenic metabolites during DMBA-induced hamster buccal pouch carcinogenesis.

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Modulating Effect of Lupeol on the Expression Pattern of Apoptotic Markers in 7, 12-Dimethylbenz(a)anthracene Induced Oral Carcinogenesis

Cited by 27 publications

References 27 publications

Modulating effect of Enicostemma littorale on the expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers During 7, 12-Dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis

Modulating effect of Enicostemma littorale on the expression pattern of apoptotic, cell proliferative, inflammatory and angiogenic markers During 7, 12-Dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis

Parkia speciosaHassk.: A Potential Phytomedicine

Saffron Reduction of 7,12-Dimethylbenz[a]anthracene-induced Hamster Buccal Pouch Carcinogenesis

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