Modulating size and surface charge of ethylcellulose nanoparticles through the use of cationic nano-emulsion templates

Calderó, Gabriela; Leitner, S.; García-Celma, María José; Solans, Conxita

doi:10.1016/j.carbpol.2019.115201

Cited by 10 publications

(10 citation statements)

References 39 publications

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“…As shown in Figure 2b, the nano-emulsions formed display typically a translucent to transparent appearance, depending on the O/S ratio and the HEPES 20 mM content, with a bluish shine due to the Tyndall effect. This appearance is strikingly different from previously reported systems prepared with the same cationic surfactant (CatA) but different nonionic surfactants (namely Span 80 and Cremophor WO7) which showed higher opacity (Leitner et al 2019;Calderó et al 2019). It is also worth mentioning that in the system described herein, the nano-emulsion domain is considerably larger than in the latter mentioned systems.…”

Section: Transfection Efficiency Assaycontrasting

confidence: 96%

“…This value is much higher than expected for a nano-emulsion with 95 wt% aqueous component. In earlier reported systems, the shrinking factor of nano-emulsions at this aqueous component content was close to 1, that is, the volume of the nanoparticle and that of the nano-emulsion drop were very similar (Calderó et al 2019). The rather high shrinking factor in the current system suggests that the interfacial film in this nano-emulsion might be more efficient in avoiding solvent diffusion from the dispersed drop to the continuous phase along the dilution path followed for its preparation, in spite of the more favorable osmotic gradient expected with HEPES 20 mM buffer solution as compared to water.…”

Section: Transfection Efficiency Assaymentioning

confidence: 54%

“…As for bottom-up techniques, the low-energy emulsification approach is an environmentally friendly method, which has been reported for the preparation of ethylcellulose nanoparticles (Spernarth et al 2007;Generalova et al 2009;Calderó et al 2011). Although ethylcellulose is nonionic, recently it has been shown that positively charged ethylcellulose nanoparticle dispersions can be obtained through nano-emulsion templating in cationic:non-ionic surfactant-based systems (Leitner et al 2019;Calderó et al 2019). These oil-in-water (O/W) nano-emulsion templates are obtained by a low-energy method and the positive surface charge of the dispersed systems is attributed to the cationic surfactant.…”

Section: Introductionmentioning

confidence: 99%

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Ethylcellulose nanoparticles as a new “in vitro” transfection tool for antisense oligonucleotide delivery

Leitner

Grijalvo

Solans

et al. 2020

Carbohydrate Polymers

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Oil-in-water nano-emulsions have been obtained in the HEPES 20 mM buffer solution / [Alkylamidoammonium:Kolliphor EL=1:1] / [6 weight % ethylcellulose in ethyl acetate] system over a wide oil-to-surfactant (O/S) range and above 35 weight% aqueous component at 25ºC. The nano-emulsion with an O/S ratio of 70/30 and 95 weight % aqueous component was used for nanoparticles preparation. These nanoparticles (mean diameter around 90 nm and zeta potential of +22 mV) were non-toxic to HeLa cells up to a concentration of 3 mM of cationic species. Successful complexation with an antisense phosphorothioate oligonucleotide targeting Renilla luciferase mRNA was achieved at cationic/anionic charge ratios above 16, as confirmed by zeta potential measurements and an electrophoretic mobility shift assay, provided that no Fetal Bovine Serum is present in the cell culture medium. Importantly, Renilla luciferase gene inhibition shows an optimum efficiency (40%) for the cationic/anionic ratio 28, which makes these complexes promising for "in vitro" cell transfection.

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Section: Transfection Efficiency Assaycontrasting

confidence: 96%

Section: Transfection Efficiency Assaymentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Ethylcellulose nanoparticles as a new “in vitro” transfection tool for antisense oligonucleotide delivery

Leitner

Grijalvo

Solans

et al. 2020

Carbohydrate Polymers

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“…Since there is an increasing interest to employ processes that are environmentally sustainable, safe and energy-saving, the solvent displacement method has been widely used. This method enables the production of nanospheres as well as nanocapsules in one cost-efficient process, with a high yield encapsulation of hydrophobic compounds [ 5 ]. The nanoprecipitation method requires three basic ingredients to be performed: the polymer, the solvent and the antisolvent (also known as non-solvent) of the polymer.…”

Section: Introductionmentioning

confidence: 99%

Bio-Based Nanoparticles as a Carrier of β-Carotene: Production, Characterisation and In Vitro Gastrointestinal Digestion

et al. 2020

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β-carotene loaded bio-based nanoparticles (NPs) were produced by the solvent-displacement method using two polymers: zein and ethylcellulose. The production of NPs was optimised through an experimental design and characterised in terms of average size and polydispersity index. The processing conditions that allowed to obtain NPs (<100 nm) were used for β-carotene encapsulation. Then β-carotene loaded NPs were characterised in terms of zeta potential and encapsulation efficiency. Transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction analysis were performed for further morphological and chemical characterisation. In the end, a static in vitro digestion following the INFOGEST protocol was performed and the bioaccessibility of β-carotene encapsulated in both NPs was determined. Results show that the best conditions for a size-controlled production with a narrow size distribution are lower polymer concentrations and higher antisolvent concentrations. The encapsulation of β-carotene in ethylcellulose NPs resulted in nanoparticles with a mean average size of 60 ± 9 nm and encapsulation efficiency of 74 ± 2%. β-carotene loaded zein-based NPs resulted in a mean size of 83 ± 8 nm and encapsulation efficiency of 93 ± 4%. Results obtained from the in vitro digestion showed that β-carotene bioaccessibility when encapsulated in zein NPs is 37 ± 1%, which is higher than the value of 8.3 ± 0.1% obtained for the ethylcellulose NPs.

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“…Nanoparticles of positive surface charge could be depleted via diluting circumstances over time. Calderó, Leitner, García-Celma, and Solans (2019) The correlation between emulsion characteristics and produced electrosprayed nanoparticles…”

Section: Isoniazidmentioning

confidence: 99%

Evaluating the structural properties of bioactive‐loaded nanocarriers with modern analytical tools

Rostamabadi

Falsafi

Assadpour

et al. 2020

Comp Rev Food Sci Food Safe

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With the emergence of nanocarriers for offering smart transformers improving the bioavailability and functionality of food bioactive compounds, a critical issue is experimental and analytical evaluation of the bioactive-loaded nanocarriers; so, further technical advancements are necessary for the precise structural characterization of these nanodelivery systems and application of sophisticated analytical techniques. Here, we have portrayed the current progress in the structural characterization approaches including X-ray diffraction, differential scanning calorimetry, thermogravimetric analysis, fluorescence spectroscopy, circular dichroism, Fourier-transform infrared spectroscopy, nuclear magnetic resonance, X-ray photoelectron spectroscopy, and small-angle X-ray scattering/small-angle neutron scattering as powerful and informative tools to have a better control on the design, development, and appraisal of nanoengineered bioactive delivery systems. Different examples and case studies have been tabulated along with illustrative and schematic representation of relevant results. An emerging view concerning the challenges for future structural characterization of these systems is also discussed in detail.

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Modulating size and surface charge of ethylcellulose nanoparticles through the use of cationic nano-emulsion templates

Cited by 10 publications

References 39 publications

Ethylcellulose nanoparticles as a new “in vitro” transfection tool for antisense oligonucleotide delivery

Ethylcellulose nanoparticles as a new “in vitro” transfection tool for antisense oligonucleotide delivery

Bio-Based Nanoparticles as a Carrier of β-Carotene: Production, Characterisation and In Vitro Gastrointestinal Digestion

Evaluating the structural properties of bioactive‐loaded nanocarriers with modern analytical tools

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