Modulating the Antifungal Activity of Antimycotic Drugs with Farnesol

Sachivkina, Nadezhda; Senyagin, Alexander; Podoprigora, I. V.; Brown, D. G.; Vissarionova, V. V.

doi:10.33380/2305-2066-2021-10-4-162-168

Cited by 3 publications

(5 citation statements)

References 16 publications

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“…Regardless of the fungal strains, we observed that the inhibition diameters decreased with a decrease in the concentration of the AgNPs, which may indicate that the synthesized AgNPs are dose-dependent. Similar results are usually reported in most studies investigating the antimicrobial properties of nanoparticles and other drugs [ 2 , 24 , 34 – 37 ]. At lower amounts of AgNPs (5 μg/mL), all the clinical strains (C1, C2, C3, and C4) were not sensitive (0 mm), while at the same concentration, an inhibition zone of 10 mm was found against the reference strains C. albicans ATCC 10231.…”

Section: Resultssupporting

confidence: 89%

Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans

et al. 2023

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Background and Aim: Resistance to antifungal agents is a serious public health concern that has not been investigated enough because most studies on antimicrobials are dedicated to antibacterial resistance. This study aimed to synthesize silver nanoparticles (AgNPs) using Aloe vera extract, and to assess its antifungal activity against Candida albicans. Materials and Methods: Silver nanoparticles were synthesized by reducing Ag nitrate with aqueous A. vera extracts. Physicochemical properties of synthesized AgNPs were determined by ultraviolet–visible spectrophotometry, photon cross-correlation spectroscopy, energy-dispersive X-ray fluorescence spectrometry, X-ray diffraction analysis, and Fourier-transform infrared spectroscopy. An antifungal investigation was performed against four clinical C. albicans (C1, C2, C3, and C4) and a reference strain, C. albicans ATCC 10321. Results: Cubic AgNPs with a mean X50 hydrodynamic diameter of 80.31 ± 10.03 nm were successfully synthesized. These AgNPs exhibited maximum absorbance at 429.83 nm, and X-ray fluorescence (XRF) confirmed the presence of Ag in AgNPs solution by a characteristic peak in the spectrum at the Ag Kα line of 22.105 keV. Infrared spectra for AgNPs and A. vera extract indicated that the compounds present in the extract play an essential role in the coating/capping of synthesized AgNPs. Different concentrations (200, 100, 50, 25, 10, and 5 μg/mL) of AgNPs were tested. The antifungal activity was shown to be dose-dependent with inhibition zones ranging from 10 mm to 22 mm against C. albicans ATCC 10231, 0 mm to 15 mm against C1, 0 mm to 16 mm against C2 and C3, and 0 mm to 14 mm for C4. Minimum inhibitory concentration ranged from 16 μg/mL to 32 μg/mL against clinical C. albicans (C1, C2, C3, and C4) and was 4 μg/mL against C. albicans ATCC 10231. Conclusion: This study showed the ability of A. vera to serve as an efficient reducing agent for the biogenic synthesis of AgNPs with excellent antifungal activity.

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Section: Resultssupporting

confidence: 89%

Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans

et al. 2023

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“…Much research has been devoted to studying the role of Far as an inhibitor of hyphal morphogenesis [ 41 , 43 ]; however, little research has been published regarding the impacts of Far on fungal virulence and the development of M. pachydermatis biofilms.…”

Section: Discussionmentioning

confidence: 99%

“…The sensitivity of BY M. pachydermatis to antimycotic drugs was investigated using the Kirby–Bauer’s disk diffusion method, exactly as described in our previous study [ 41 , 42 , 43 ]. The antimycotics tested were nystatin (NS; 50 µg), amphotericin B (AP; 10 µg), ketoconazole (KT; 10 µg), clotrimazole (CC; 10 µg), voriconazole (VOR; 10 µg), fluconazole (FU; 25 µg), miconazole (MIC; 10 µg), and intraconazole (IT; 10 µg) (HiMedia™ Laboratories Pvt.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Activity of Farnesol against Malassezia pachydermatis Isolates from Otitis Externa Cases in Dogs

Olabode

Sachivkina

Karamyan

et al. 2023

Animals

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Chronic otitis externa of dogs is a significant problem due to the prevalence and complexity of the treatment of such animals. There is evidence that in 60–80% of cases of infectious diseases microorganisms located in the biofilm phenotype play the main role. Microorganisms in the biofilm phenotype have a number of advantages, the most significant of which is considered to be increased resistance to various external factors. Among them, a special place is occupied by resistance to antibiotics. In recent decades, research has been conducted at an increasing scale on the role of biofilm infections in various pathologies in veterinary medicine. The etiology and therapy of dog otitis externa caused by Malassezia pachydermatis biofilm has not been fully studied. This is why we consider relevant the scientific and practical aspects of research on the etiology and therapy of dog otitis externa from the position of biofilm infection. In this work, it has been statistically proven that there is a relationship between the optical density of Malassezia pachydermatis biofilms and their sensitivity to drugs, and this relationship is statistically significant. In addition, we have demonstrated that Farnesol has a good antibiofilm effect at a concentration of more 1.6 μM/mL (24% OD decrease of biofilm), and its highest antibiofilm effect (71–55%—more than a half) was observed at a concentration of 200–12.5 μM/mL.

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“…Due to the fact that YLF of Malassezia genus often enter into symbiotic relationships with pathogenic and opportunistic bacteria, Farnesol therapy is of particular interest, since the antibacterial activity of this drug has long been recognized worldwide [14][15][16][17][18][19][20][33][34][35][36]. Thus, the local application of Farnesol in polyinfection may have an effect on several microorganisms at once.…”

Section: Indicatorsmentioning

confidence: 99%

“…Recently, in the scientific literature, close attention has been paid to the study of herbal medicines, the possibility of their use for the treatment of infectious (fungal and bacterial) diseases [12,13]. According to the literature and our own research, Farnesol (C 15 H 26 O) has proven its antimycotic efficacy in in vivo and in vitro models [14][15][16][17][18][19]. Chemically, Farnesol is an acyclic sesquiterpene alcohol, is a thermally stable molecule that is not exposed to extreme pH values, which is especially important in the development of YLF infection.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Farnesol for treatment of dog otitis complicated by Malassezia pachydermatis

Olabode,

Sachivkina,

Kiseleva

et al. 2023

Vestn. Ross. univ. družby nar., Ser. Agron. životnovod.

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The study involved 30 dogs with otitis complicated by Malassezia pachydermatis . The animals were divided into 2 groups: experimental (n = 15) and control (n = 15). The experimental group was prescribed veterinary ear drops Surolan + a solution of the drug Farnesol; the control group - Surolan. The study showed that when Farnesol is added to the treatment regimen for fungal otitis etiology in dogs, small changes in the clinical composition of the blood are recorded, characterized by an increase in the number of erythrocytes and hemoglobin, a decrease in the number of leukocytes, with a decrease in the indicators of eosinophils and rod-shaped neutrophils in the experiment compared with the control. In addition, the complete absence of Malassezia pachydermatis in the smears of the ear contents after two weeks of therapy with a combined drug was proved. That is, in the experimental group, there were no cases of the presence of yeast-like fungi (YLF) in the smear after combined therapy with Surolan + Farnesol. And in the control group of 15 animals, YLF were observed in two with microscopy of ear exudate smears, but not in high concentration. The use of a combination of Surolan and Farnesol in dogs of the experimental group led to a decrease in hyperemia, itching, edema, ear fetid fluid on the 5th7th days of treatment, and complete clinical recovery of the animals occurred by the 10th14th day of therapy. In the control group, only Surolan was used, and improvement of clinical condition occurred on days 1214, and recovery only after completion of the full course. As a result, both treatment regimens were successful, but the scheme with Farnesol gave faster results due to the wide spectrum of action of this drug: both antimicrobial and anti-inflammatory.

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Modulating the Antifungal Activity of Antimycotic Drugs with Farnesol

Cited by 3 publications

References 16 publications

Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans

Antifungal activity of silver nanoparticles prepared using Aloe vera extract against Candida albicans

In Vitro Activity of Farnesol against Malassezia pachydermatis Isolates from Otitis Externa Cases in Dogs

Effectiveness of Farnesol for treatment of dog otitis complicated by Malassezia pachydermatis

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