Modulating the Biofunctionality of Metal–Organic‐Framework‐Encapsulated Enzymes through Controllable Embedding Patterns

Abstract

Embedding an enzyme within aMOF as exoskeleton (enzyme@MOF) offers new opportunities to improve the inherent fragile nature of the enzyme,but also to impart novel biofunctionality to the MOF.D espite the remarkable stability achieved for MOF-embedded enzymes,e mbedding patterns and conversion of the enzymatic biofunctionality after entrapment by aM OF have only received limited attention. Herein, we reveal howe mbedding patterns affect the bioactivity of an enzyme encapsulated in ZIF-8. The enzyme@MOF can maintain high activity when the encapsulation process is driven by rapid enzyme-triggered nucleation of ZIF-8. When the encapsulation is driven by slow coprecipitation and the enzymes are not involved in the nucleation of ZIF-8, enzy-me@MOF tends to be inactive owing to unfolding and competing coordination caused by the ligand, 2-methyl imidazole.T hese two embedding patterns can easily be controlled by chemical modification of the amino acids of the enzymes,modulating their biofunctionality.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

“Armor‐Plating” Enzymes with Metal–Organic Frameworks (MOFs)

“… Copyright 2019 American Chemical Society; (b,c) reprinted with permission from Ref. Copyright 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim).…”

“…A very recent findings by our group showed that the encapsulation patterns also played a vital role in changing the enzymatic activity (Figure b) . When the encapsulation process was driven by the rapid enzyme‐triggered nucleation of ZIF‐8, the entrapped enzymes within ZIF‐8 maintained a high activity.…”

“…Owing to the accelerating effect caused by the high local concentrations of the substrates at the intercommunicating enzymes encapsulated in the ZIF‐8, the sensor showed excellent sensitivity for glucose detection, and with much higher stability compared to the sensing system in solution. This MOF‐confined enzymatic sensor was also applied for naked‐eye detection of biothiols based on a competing reaction mechanism . As well as for colorimetric sensing, robust enzyme@MOFs biocomposites were also proposed to fabricate ultra‐stable modified‐electrode for electrochemical biosensing .…”

Metal‐Organic Frameworks: A New Platform for Enzyme Immobilization

“…In addition, in the case that the enzymes with positive charges are not able to induce the MOFs spontaneous formation, changing the charges of proteins from positive to negative by surface chemical modification could realize the biomineralization process. [19] And the enzymes encapsulated within MOFs by this improved method could well maintain their activity after the immobilization. Biomineralized enzyme@MOFs composites were now received increasing attentions in different research fields such as biocatalysis and biomedicine.…”

“Panzerung” von Enzymen mit Metall‐organischen Gerüsten

“…, Copyright 2019 American Chemical Society; Wiedergabe von (b, c) mit Genehmigung aus Lit. , Copyright 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.…”