Modulating the expression of survivin and other basal epidermal proteins protects human skin from <scp>UVB</scp> damage and oxidative stress

Labarrade, F.; Bergeron, L.; Serre, C.; Lebleu, A.; Busuttil, V.; Botto, J.; Domloge, N.

doi:10.1111/jocd.12150

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…This environment was progressively characterized by defined markers, such as α6 integrin, β1 integrin, keratin 15, and survivin. 9,10 Skin renewal process rely on skin stem cells but also on their differentiation behaviors. Defect in the differentiation process and changes in epidermis thickness lead to several tissue disorders, and a compromised skin barrier.…”

Section: Introductionmentioning

confidence: 99%

“…Keratinocyte stem cells are located at the deepest point in the epidermis, at the base of the epidermal ridge or peg 6–8 . Keratinocyte stem cells reside in a special microenvironment called the “niche.” This environment was progressively characterized by defined markers, such as α6 integrin, β1 integrin, keratin 15, and survivin 9,10 . Skin renewal process rely on skin stem cells but also on their differentiation behaviors.…”

Section: Introductionmentioning

confidence: 99%

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miR‐203 represses keratinocyte stemness by targeting survivin

Labarrade

Botto

Imbert

2022

J of Cosmetic Dermatology

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Objective The epidermis possesses the capacity to replace dying cells and to heal wounds, thanks to resident stem cells, which have self‐renewal properties. In skin physiology, miRNAs have been shown to be involved in many processes, including skin and hair morphogenesis. Recently, differentiation of epidermal stem cells was shown to be promoted by the miR‐203. The miR‐203 is upregulated during epidermal differentiation and is of interest because of significant targets. Methods By utilizing a bioinformatic tool, we identified a target site for miR‐203 in the survivin mRNA. Silencing miR‐203 was managed with the use of antagomir; the silencing of survivin was performed with a siRNA. Survivin expression was determined by qPCR or immunofluorescence in cultured cells, and by immunohistochemistry in skin sections. Involucrin expression was used as marker of keratinocyte differentiation. A rice extract with previously demonstrated anti‐aging properties was evaluated on miR‐203 modulation. Results In this study, we identified a miR‐203/survivin axis, important for epidermal homeostasis. We report that differentiation of keratinocyte is dependent on the level of miR‐203 expression and that inhibition of miR‐203 can increase the expression of survivin, an epidermal marker of stemness. Conclusion In summary, our findings suggest that miR‐203 target 3′UTR region of survivin mRNA and directly represses survivin expression in the epidermis. The rice extract was identified as modulator of miR‐203 and pointed out as a promising microRNA‐based strategy in treating skin changes occurring with aging.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

miR‐203 represses keratinocyte stemness by targeting survivin

Labarrade

Botto

Imbert

2022

J of Cosmetic Dermatology

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Survivin expression in head and neck squamous cell carcinomas is frequent and correlates with clinical parameters and treatment outcomes

et al. 2018

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Effect of Ceramides Derivatives from the Peach on Skin Function Improvement in UV-Irradiated Hairless Mice

Kim,

Lee,

Cho

et al. 2024

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This study investigated the protective effects of a ceramides derivates from the peach (PF3) on photoaging by UV-irradiated hairless mice. Mice were randomly divided into seven groups: AIN93G without UVB exposure (normal control, NC), AIN93G with UVB exposure (control, C), AIN93G supplemented 100 mg/kg body weight (BW) of L-ascorbic acid with UVB exposure (AA), AIN93G supplemented 100 mg/kg BW of arbutin with UVB exposure (Arbutin), AIN93G supplemented 10 mg/kg BW of PF3 with UVB exposure (10PF3), AIN93G supplemented 20 mg/kg BW of PF3 with UVB exposure (20PF3), and AIN93G supplemented 40 mg/kg BW of PF3 with UVB exposure (40PF3). The study examined the impact of PF3 on skin hydration, wrinkle formation, and melanogenesis using enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (real-time PCR), and Western blot analysis. The PF3 demonstrated significant protective effects against photoaging by reducing skin wrinkle formation, decreasing epidermal and dermal thickening, and improving skin hydration. It also enhanced the expression of moisture-related factors (hyaluronic acid synthase [HAS], long-chain ceramides [LCBs], dihydroceramide desaturase 1 [DEGS1], and type I collagen [COL1A]) and antioxidant enzyme activities while reducing pro-inflammatory cytokines and oxidative stress markers. The PF3 supplementation positively modulated skin wrinkle formation-related factors, increasing collagen-related gene expression and decreasing matrix metalloproteinases. Additionally, PF3 showed potential in regulating melanogenesis by reducing the nitric oxide and cAMP content, as well as the expression of melanogenesis-related proteins. These comprehensive findings suggest that PF3 supplementation may be an effective strategy for preventing and treating UVB-induced skin photoaging through multiple mechanisms, including improved skin structure, hydration, antioxidant defense, and reduced inflammation and pigmentation.

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Modulating the expression of survivin and other basal epidermal proteins protects human skin from UVB damage and oxidative stress

Abstract: Compound IV08.009 demonstrated to be effective in regulating survivin expression and in preserving the basal epidermis from stresses such as UVB and H2 O2 . These results suggest a protective activity of IV08.009 on the essential renewing potential of KSCs.

Cited by 3 publications

References 29 publications

miR‐203 represses keratinocyte stemness by targeting survivin

miR‐203 represses keratinocyte stemness by targeting survivin

Survivin expression in head and neck squamous cell carcinomas is frequent and correlates with clinical parameters and treatment outcomes

Effect of Ceramides Derivatives from the Peach on Skin Function Improvement in UV-Irradiated Hairless Mice

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