Modulating the phenotype of host macrophages to enhance osteogenesis in MSC-laden hydrogels: Design of a glucomannan coating material

Niu, Yiming; Li, Qiu; Xie, Ruiyu; Liu, Shang; Wang, Ruibing; Xing, Panfei; Shi, Yundi; Wang, Ying; Dong, Lei; Wang, Chunming

doi:10.1016/j.biomaterials.2017.05.042

Cited by 74 publications

(60 citation statements)

References 48 publications

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“…141 Besides, coating of macrophage-affinitive glucomannan enhances the bone regenerative performance of 3D hydrogel scaffolds. 142 MiRNAs are being developed to enhance tissue regeneration, because they can downregulate or upregulate the expression of their target genes. 143 Zhang et al showed that miR-26a increased osteoblast activities by functionally targeting Gsk-3β in bone repair.…”

Section: Controlled Release Of Active Chemical Components For Bone Tementioning

confidence: 99%

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

2020

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Natural bone is a mineralized biological material, which serves a supportive and protective framework for the body, stores minerals for metabolism, and produces blood cells nourishing the body. Normally, bone has an innate capacity to heal from damage. However, massive bone defects due to traumatic injury, tumor resection, or congenital diseases pose a great challenge to reconstructive surgery. Scaffold-based tissue engineering (TE) is a promising strategy for bone regenerative medicine, because biomaterial scaffolds show advanced mechanical properties and a good degradation profile, as well as the feasibility of controlled release of growth and differentiation factors or immobilizing them on the material surface. Additionally, the defined structure of biomaterial scaffolds, as a kind of mechanical cue, can influence cell behaviors, modulate local microenvironment and control key features at the molecular and cellular levels. Recently, nano/micro-assisted regenerative medicine becomes a promising application of TE for the reconstruction of bone defects. For this reason, it is necessary for us to have in-depth knowledge of the development of novel nano/micro-based biomaterial scaffolds. Thus, we herein review the hierarchical structure of bone, and the potential application of nano/micro technologies to guide the design of novel biomaterial structures for bone repair and regeneration.

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Section: Controlled Release Of Active Chemical Components For Bone Tementioning

confidence: 99%

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

2020

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“…12 Therefore, a new strategy constituting a more physiologically relevant way of improving the performance of engineered tissues may involve utilizing the power of macrophages. 13 However, few studies have investigated how the interactions between biomaterials and immune cells affect the subsequent osteogenesis and angiogenesis of seeding cells.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis

Xue

Chen

Zhong

et al. 2018

IJN

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BackgroundThe osteo-immunomodulatory properties of biomaterials play an important role in the outcomes of bone regeneration. Graphene oxide (GO) has been widely applied in many research fields due to its unique properties. However, the immunomodulatory properties of GO as a biomaterial for bone tissue engineering are still unclear.Materials and methodsIn this study, we evaluated the Inflammatory response of RAW264.7 cells influenced by GO. Then the osteogenic differentiation of BMSCs, and angiogenic differentiation of human umbilical vein endothelial cells (HUVECs) by stimulation with GO/RAW 264.7-conditioned culture medium were accessed. We also further investi gated the possible mechanisms underlying the osteo- and angio-immunomodulatory effects of GO.ResultsOur results showed that GO stimulates the secretion of oncostatin M, tumor necrosis factor alpha and other factors through the nuclear factor-κB pathway. GO/RAW264.7-conditioned medium promoted the osteogenic differentiation of BMSCs, stimulated upregulation of the HUVECs of vascular-related receptors, and promoted their tube formation in vitro.ConclusionIn conclusion, our research shows that GO, as a biomaterial, can induce the formation of a beneficial osteo-immunomodulatory environment and is a promising biomaterial for bone tissue engineering.

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“…In regard to wound healing and regeneration, harnessing the host macrophages to enhance the differentiation of delivered cells has become a good strategy for regenerative medicine. Niu et al ( 2017 ) recently designed a new acetyl Bletilla striata polysaccharide (acBSP) polymer coating, which was able to promote the activation of tissue macrophages at the host-scaffold interface to secrete pro-regenerative cytokines that can enhance the osteogenesis of the mesenchymal stem cells inside the scaffold (Niu et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Immune Assisted Tissue Engineering via Incorporation of Macrophages in Cell-Laden Hydrogels Under Cytokine Stimulation

Barthès

Dollinger

Muller

et al. 2018

Front. Bioeng. Biotechnol.

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The function of soft tissues is intricately linked to their connections with the other systems of the body such as circulation, nervous system, and immune system. The presence of resident macrophages in tissues provides a means to control tissue homeostasis and also a way to react to the physical/biological insults and tissue damage. Thus, incorporation of resident macrophage like phenotype-controlled macrophages in engineered tissues can improve their fidelity as model tissues and also improve their rate of integration and facilitate the resolution of inflammation for regenerative medicine applications. Herein, we demonstrate two potential ways to immunoassist the remodeling process of engineered soft tissues in three-dimensional (3-D) gelatin based hydrogels containing fibroblasts and/or endothelial cells: (i) with supplementation of interleukin-4 (IL-4) in the presence of macrophages and (ii) in tri-culture via naive monocytes or differentiated macrophages. The presence of IL-4 had a proliferative effect on fibroblasts, with a significant boosting effect on proliferation and cytokine secretion in the presence of differentiated macrophages with an upregulation of activin, interleukin-1 receptor antagonist (IL-1RA), tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β), creating a more stimulating microenvironment. The addition of IL-4 in endothelial cell/macrophage co-culture configuration improved the organization of the sprout-like structures, with a boost in proliferation at day 1 and with an upregulation of IL-6 and IL-1RA at the earliest stage in the presence of differentiated macrophages creating a favorable microenvironment for angiogenesis. In tri-culture conditions, the presence of monocytes or macrophages resulted in a denser tissue-like structure with highly remodeled hydrogels. The presence of differentiated macrophages had a boosting effect on the angiogenic secretory microenvironment, such as IL-6 and IL-8, without any additional cytokine supplementation. The presence of fibroblasts in combination with endothelial cells also had a significant effect on the secretion of angiopoietin. Our results demonstrate that incorporation of macrophages in a resident macrophage function and their phenotype control have significant effects on the maturation and cytokine microenvironment of 3-D multiple cell type-laden hydrogels, which can be harnessed for better integration of implantable systems and for more physiologically relevant in vitro tissue models with an immune component.

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Modulating the phenotype of host macrophages to enhance osteogenesis in MSC-laden hydrogels: Design of a glucomannan coating material

Cited by 74 publications

References 48 publications

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

Immunomodulatory properties of graphene oxide for osteogenesis and angiogenesis

Immune Assisted Tissue Engineering via Incorporation of Macrophages in Cell-Laden Hydrogels Under Cytokine Stimulation

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