Modulating Vesicle Adhesion by Electric Fields

Steinkühler, Jan; Agudo-Canalejo, Jaime; Lipowsky, Reinhard; Dimova, Rumiana

doi:10.1016/j.bpj.2016.08.029

Cited by 30 publications

(30 citation statements)

References 48 publications

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“…Under a strong adhesion, however, the vesicle membranes in the contact region buckle and form a sigmoidal shape that has also been observed in RBC doublets [29,30,33]. An external electric field is also able to buckle a vesicle membrane that is adhered to a solid substrate [54].…”

Section: B Effects Of the Adhesion Parameters On Equilibrium Configumentioning

confidence: 74%

Hydrodynamics and rheology of a vesicle doublet suspension

2019

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The dynamics of an adhesive two-dimensional vesicle doublet under various flow conditions is investigated numerically using a high-order, adaptive-in-time boundary integral method. In a quiescent flow, two nearby vesicles move slowly towards each other under the adhesive potential, pushing out fluid between them to form a vesicle doublet at equilibrium. A lubrication analysis on such draining of a thin film gives the dependencies of draining time on adhesion strength and separation distance that are in good agreement with numerical results. In a planar extensional flow we find a stable vesicle doublet forms only when two vesicles collide head-on around the stagnation point. In a microfluid trap where the stagnation of an extensional flow is dynamically placed in the middle of a vesicle doublet through an active control loop, novel dynamics of a vesicle doublet are observed. Numerical simulations show that there exists a critical extensional flow rate above which adhesive interaction is overcome by the diverging stream, thus providing a simple method to measure the adhesion strength between two vesicle membranes. In a planar shear flow, numerical simulations reveal that a vesicle doublet may form provided that the adhesion strength is sufficiently large at a given vesicle reduced area. Once a doublet is formed, its oscillatory dynamics is found to depend on the adhesion strength and their reduced area. Furthermore the effective shear viscosity of a dilute suspension of vesicle doublets is found to be a function of the reduced area. Results from these numerical studies and analysis shed light on the hydrodynamic and rheological consequences of adhesive interactions between vesicles in a viscous fluid.Keywords: Vesicle hydrodynamics, membrane adhesion, drop and bubble phenomena/drop interactions, interfacial flows/thin fluid films, rheology/flow confinement, biological fluid dynamics/collective behavior/clustering, emergence of patterns

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Section: B Effects Of the Adhesion Parameters On Equilibrium Configumentioning

confidence: 74%

Hydrodynamics and rheology of a vesicle doublet suspension

2019

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“…[37,38] Although surface immobilization methods are straightforward, they do have drawbacks. [40,41] Complete spatial control is more effectively achieved using microfabricated structures as explained in the following section. Moreover, unwanted nanotubes can also result from adhered vesicles subjected to flow, but this can be used to an advantage.…”

Section: Surface Tetheringmentioning

confidence: 99%

“…[39] Finally, adhesion to a surface is also known to have adverse effects on membrane behavior and properties so alternative options should always be considered. [40,41] Complete spatial control is more effectively achieved using microfabricated structures as explained in the following section.…”

Section: Surface Tetheringmentioning

confidence: 99%

Microfluidic Handling and Analysis of Giant Vesicles for Use as Artificial Cells: A Review

Robinson

2019

Adv. Biosys.

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One of the goals of synthetic biology is the bottom‐up construction of an artificial cell, the successful realization of which could shed light on how cellular life emerged and could also be a useful tool for studying the function of modern cells. Using liposomes as biomimetic containers is particularly promising because lipid membranes are biocompatible and much of the required machinery can be reconstituted within them. Giant lipid vesicles have been used extensively in other fields such as biophysics and drug discovery, but their use as artificial cells has only recently seen an increase. Despite the prevalence of giant vesicles, many experiments remain challenging or impossible due to their delicate nature compared to biological cells. This review aims to highlight the effectiveness of microfluidic technologies in handling and analyzing giant vesicles. The advantages and disadvantages of different microfluidic approaches and what new insights can be gained from various applications are introduced. Finally, future directions are discussed in which the unique combination of microfluidics and giant lipid vesicles can push forward the bottom‐up construction of artificial cells.

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“…[27] A prime example of spatiotemporal control over cell-matrix adhesions is observed during cell motility. Biomimetic adhesions that can be triggered with an external stimulus provide similarly temporal and/or spatial control.…”

Section: Stimuli Responsive Synthetic Cell To Matrix Adhesionmentioning

confidence: 99%

“…For instance, the adhesion of a GUV rich in negatively charged lipids can be induced at any given point in time using an external electric field and can be reversed by turning the trigger off. [27] A prime example of spatiotemporal control over cell-matrix adhesions is observed during cell motility. [18] As the cell moves forward, it assembles new adhesions at the leading edge at the same rate as it disassembles them at the trailing edge.…”

Section: Stimuli Responsive Synthetic Cell To Matrix Adhesionmentioning

confidence: 99%

Mimicking Adhesion in Minimal Synthetic Cells

et al. 2019

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Cell adhesions to the extracellular matrix and to neighboring cells are fundamental to cell behavior and have also been implemented into minimal synthetic cells, which are assembled from molecular building blocks from the bottom‐up. Investigating adhesion in cell mimetic models with reduced complexity provides a better understanding of biochemical and biophysical concepts underlying the cell adhesion machinery. In return, implementing cell–matrix and cell–cell adhesions into minimal synthetic cells allows reconstructing cell functions associated with cell adhesions including cell motility, multicellular prototissues, fusion of vesicles, and the self‐sorting of different cell types. Cell adhesions have been mimicked using both the native cell receptors and reductionist mimetics providing a variety of specific, reversible, dynamic, and spatiotemporally controlled interactions. This review gives an overview of different minimal adhesion modules integrated into different minimal synthetic cells drawing inspiration from cell and colloidal science.

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Modulating Vesicle Adhesion by Electric Fields

Cited by 30 publications

References 48 publications

Hydrodynamics and rheology of a vesicle doublet suspension

Hydrodynamics and rheology of a vesicle doublet suspension

Microfluidic Handling and Analysis of Giant Vesicles for Use as Artificial Cells: A Review

Mimicking Adhesion in Minimal Synthetic Cells

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