Modulation by neurotensin and neuromedin N of adherence and chemotaxis capacity of murine lymphocytes

Garrido, Juan J.; Arahuetes, R.M.; Hernánz, Ángel; Fuente, Mónica De la

doi:10.1016/0167-0115(92)90511-r

Cited by 41 publications

(17 citation statements)

References 24 publications

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“…133 NT interacts with neutrophils affecting several neutrophil functions, including locomotion, phagocytosis, and adherence. 145 NT also increases chemotaxis of lymphocytes 146 and interacts with macrophages, stimulating their phagocytic activity, 147 and production of IL-1␤. 148 NT, via NTR1, binds to human umbilical vascular endothelial cells, 149 stimulating release of prostacyclin 150 and enhancing their migration, implicating a role for NT in angiogenesis.…”

Section: Nt Mediates Inflammatory Responses In the Small Intestine Anmentioning

confidence: 99%

Role of neuropeptides in inflammatory bowel disease

Gross

Pothoulakis

2007

Inflammatory Bowel Diseases

149

128

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Inflammatory bowel disease (IBD) is a chronic, relapsing condition involving complex interactions between genes and the environment. The mechanisms triggering the initial attack and relapses, however, are not well understood. In the past several years the enteric nervous system (ENS) has been implicated in the pathophysiology of IBD. Both the ENS and the central nervous system (CNS) can amplify or modulate aspects of intestinal inflammation through secretion of neuropeptides that serve as a link between the ENS and CNS. Neuropeptides are defined as any peptide released from the nervous system that serves as an intercellular signaling molecule. Neuropeptides thought to play a potentially key role in IBD include substance P, corticotropin-releasing hormone, neurotensin, vasoactive intestinal peptide, mu-opioid receptor agonists, and galanin. This review focuses on the role of these neuropeptides in the pathophysiology of IBD and discusses the cell types and mechanisms involved in this process. The available evidence that neuropeptide blockade may be considered a therapeutic approach in both Crohn's disease and ulcerative colitis will also be discussed.

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Section: Nt Mediates Inflammatory Responses In the Small Intestine Anmentioning

confidence: 99%

Role of neuropeptides in inflammatory bowel disease

Gross

Pothoulakis

2007

Inflammatory Bowel Diseases

149

128

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“…The protocol used was initially established in our laboratory to study the mitogenic effects of growth factors such as somatomedins [26, 27]. This proliferative effect of NT, which was blocked by anti-NT antibody, is strongly suggestive of a receptor for NT on PBLs and is consistent with the stimulatory effect of NT on different parts of the immune system [8, 9, 10]. The improved binding achieved by preincubation with PHA, even for a short period (2 h), may indicate that NT was bound preferentially by T lymphocytes, as has been reported for growth factors [15, 34] and substance P [31], and agrees with the biological effect observed.…”

Section: Discussionmentioning

confidence: 84%

“…The influence of neurophysiological factors on the immune system was first demonstrated by the discovery of altered lymphocyte and antibody responses in animals with experimentally induced lesions in the central nervous system [6, 7]. NT has been reported to modulate lymphocyte function [8, 9, 10], and it is known to be secreted by activated human lymphocytes [11] and regulated by interleukins [12]. …”

Section: Introductionmentioning

confidence: 99%

Apparent Functionality But Impractical Quantification of Neurotensin Receptors on Human Peripheral Lymphocytes

Lhiaubet

Avard²,

Schimpff³

1998

Horm Res Paediatr

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Neurotensin (NT), a neuromodulator, is also thought to play an immunomodulatory role. We sought to confirm the effects of NT on proliferation in human peripheral blood lymphocytes (PBLs; n = 10), to characterize the binding properties of the NT receptor using Scatchard analysis, and to measure NT receptors using blood volumes (50 ml) applicable to clinical investigation. Incubation of phytohemagglutinin-stimulated PBLs with NT (10^–10 to 10^–13 M) in the presence of 0.07% normal human serum (72 h at 37°C) significantly enhanced proliferation (p < 0.001), which is consistent with PBLs possessing functional NT receptors. However, the various experimental conditions tested constantly yielded low specific NT binding to human PBLs, and hence Scatchard analysis was impossible for 50-ml blood samples. Our data confirm the existence of a link between NT and the immune system and support a physiological significance for this link. However, measurement of NT receptor binding in readily available cells proved unsuitable for clinical investigation.

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“…High-affinity neurotensin receptors have been identified on the surface of mast cells and lymphocytes, in which neurotensin binding has been shown to induce histamine release and chemotaxis, respectively [65][66][67]. Neurotensin also stimulates the release of the proinflammatory cytokine IL-8 from colonic epithelial cells through protein kinase C and NF-kB-dependent pathways [68,69].…”

Section: Neurotensin and Gastrointestinal Pathologymentioning

confidence: 98%

The role of neurotensin in physiologic and pathologic processes

Mustain

Rychahou

Evers

2011

Current Opinion in Endocrinology, Diabetes &Amp; Obesity

117

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The review highlights the wide array of biological processes in which neurotensin has a role, and summarizes the most recent advances in various fields of neurotensin research. The knowledge gained through this research has led to the development of first-in-class drugs for the treatment of various medical conditions, and it is clear that in the coming years some of these agents will be ready to move from the bench to the bedside in clinical trials.

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Modulation by neurotensin and neuromedin N of adherence and chemotaxis capacity of murine lymphocytes

Cited by 41 publications

References 24 publications

Role of neuropeptides in inflammatory bowel disease

Role of neuropeptides in inflammatory bowel disease

Apparent Functionality But Impractical Quantification of Neurotensin Receptors on Human Peripheral Lymphocytes

The role of neurotensin in physiologic and pathologic processes

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