Modulation of actin-binding and -bundling activities of MISP/Caprice by multiple phosphorylation

Maarof, Nur Diyana; Kumeta, Masahiro; Yoshimura, Shige H.

doi:10.1016/j.bbrc.2021.05.041

Cited by 1 publication

(1 citation statement)

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“…Serines 471 and 541, which are highly phosphorylated in mitotic MISP, are involved in the phosphorylation-dependent negative regulation of actin-binding activity. The phosphorylation of MISP is also critical in cells during interphase, with the phosphorylation of serines 394, 395, and 400 increasing its actin-bundling activity and inducing stress fiber formation . These studies were largely done in vitro and the associated kinases have yet to be identified, leaving open the possibility for GSK-3 involvement in these or other functions.…”

Section: Resultsmentioning

confidence: 99%

Interactomes of Glycogen Synthase Kinase-3 Isoforms

et al. 2023

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Functional differentiation of the two isoforms of the protein-serine/threonine kinase, glycogen synthase kinase-3 (GSK-3), is an unsettled area of research. The isoforms are highly similar in structure and are largely redundant, though there is also evidence for specific roles. Identification of isoform-specific protein interactors may elucidate the differences in function and provide insight into isoform-selective regulation. We therefore sought to identify novel GSK-3 interaction partners and to examine differences in the interactomes of the two isoforms using both affinity purification and proximity-dependent biotinylation (BioID) mass spectrometry methods. While the interactomes of the two isomers are highly similar in HEK293 cells, BioID in HeLa cells yielded a variety of preys that are preferentially associated with one of the two isoforms. DCP1B, which favored GSK-3α, and MISP, which favored GSK-3β, were evaluated for reciprocal interactions. The differences in interactions between isoforms may help in understanding the distinct functions and regulation of the two isoforms as well as offer avenues for the development of isoform-specific strategies.

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Section: Resultsmentioning

confidence: 99%