Modulation of acute and chronic inflammatory processes by a traditional medicine preparation GCSB-5 both in vitro and in vivo animal models

Chung, Hwa‐Jin; Lee, Hak-Sun; Shin, Joon‐Shik; Lee, Sang-Ho; Park, Byung-Mo; Youn, Yousuk; Lee, Sang Kook

doi:10.1016/j.jep.2010.05.020

Cited by 83 publications

(53 citation statements)

References 29 publications

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“…TPA is known to cause an increase in calcium influx, resulting in the activation of phospholipase D, which stimulates the conversion of arachidonic acid into prostaglandins and leukotrienes [25] and regulates the release of cytokines [26]. Furthermore, the chronic inflammation model by means of K/C administration implies that the inflammatory stimulus is maintained at least for 2 weeks, due to poor kaolin absorption; thus, tissue damage, characteristic of chronic inflammation, is established [27], involving tissue infiltration mainly by macrophages, lymphocytes, and plasma cell [28]. All of this causes tissue destruction and scarring processes involving angiogenesis, but especially fibrosis [29].…”

Section: Resultsmentioning

confidence: 99%

“…We used CD-1 female mice weighing[25][26][27][28][29][30] g each, provided by the Production and Experimentation Unit of Laboratory Animals (UPEAL) of the Metropolitan Autonomous University Xochimilco Campus (UAM Xochimilco) in Mexico City. The experiments were performed according to Official Mexican Norm 062-ZOO-1999 (Technical Specifications for the Production, Care and Use of Laboratory Animals) and the international ethical guidelines for the care and use of laboratory animals.…”

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confidence: 99%

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Effect of Hautriwaic Acid Isolated from Dodonaea viscosa in a Model of Kaolin/Carrageenan-Induced Monoarthritis

Salinas-Sánchez

Zamilpa

Pérez³

et al. 2015

Planta Med

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In the present work, the antiarthritic activity of hautriwaic acid is reported. This ent-clerodane diterpene isolated from Dodonaea viscosa was evaluated in mice using a kaolin/carrageenan-induced monoarthritis model. The inflammation observed in the joint (knee) on days 1-8 ranged from 50-70 %. After 10 days of treatment with different doses of hautriwaic acid (5, 10, 20 mg/kg), a decrease in knee inflammation was detected. This recovery was observed with both reference drugs, methotrexate (1 mg/kg) and diclofenac (0.75 mg/kg). In these groups of mice, the concentration of proinflammatory cytokines interleukin-1 beta, interleukin-6, and tumor necrosis factor alpha in the joint was significantly lower than that of the negative control group (animals with damage without any treatment). The negative control group presented a decrease in the concentration of interleukin-10, while the groups that received hautriwaic acid at different dose exhibited an increase in this interleukin. This anti-inflammatory cytokine was not modified in the joint of mice with diclofenac, but in mice that received methotrexate, a significant decrease was observed. Hautriwaic acid isolated from D. viscosa diminished the joint edema induced by this mixture of polysaccharides (carrageenan), possibly by acting as immunomodulator of the inflammatory response.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of Hautriwaic Acid Isolated from Dodonaea viscosa in a Model of Kaolin/Carrageenan-Induced Monoarthritis

Salinas-Sánchez

Zamilpa

Pérez³

et al. 2015

Planta Med

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“…Dose selection was based on a previous study (Chung et al, 2010). The animals were assigned randomly to the following groups (10 animals per group); (a) vehicle-treated sham (b) GCSB5-treated sham (c) vehicle-treated sciatic nerve transection (control), (d-g) GCSB-5 30, 100, 300, and 600 mg/kg-treated sciatic nerve transection.…”

Section: Administration Of Gcsb-5mentioning

confidence: 99%

“…GCSB-5 has been used clinically for treatment of arthritis, disc, and ankylosing spondylitis (Chung et al, 2010). It consists of a mixture of six crude drugs (root of Saposhnikovia divaricata Schiskin, root of Achyranthis bidentata Blume, bark of Acanthopanax sessiliflorum Seem, rhizome of Cibotium baromets J. Smith, seed of Glycine max Meriill, and bark of Eucommia ulmoides Oliver); each crude drug has been widely used for treatment of inflammatory diseases in Asia.…”

Section: Introductionmentioning

confidence: 99%

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Protective effect of GCSB-5, an herbal preparation, against peripheral nerve injury in rats

Kim

Yoon

Lee

et al. 2011

Journal of Ethnopharmacology

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Lipid‐soluble Extracts from Salvia miltiorrhiza Inhibit Production of LPS‐induced Inflammatory Mediators via NF‐κB Modulation in RAW 264.7 Cells and Perform Antiinflammatory Effects In Vivo

Zhang

Cai

et al. 2012

Phytotherapy Research

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Salvia miltiorrhiza, a traditional Chinese herbal medicine, is used to treat various inflammatory diseases. In the present study, the antiinflammatory effects of S. miltiorrhiza lipid-soluble extracts (SMLE) were demonstrated in vitro and in vivo, along with its underlying mechanism of action. SMLE significantly inhibited the production of NO, TNF-α, IL-1β and IL-6 in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. SMLE also inhibited the LPS-induced degradation of IκB-α in the cytoplasm and the translocation of p65 to the nucleus in RAW 264.7 cells. In addition, SMLE inhibited the production of intracellular reactive oxygen species (ROS) and the surface expression of CD14 induced by LPS. In animal models, intraperitoneal administration of SMLE increased the survival rate of endotoxemia and sepsis in mice. The topical administration of SMLE significantly inhibited ear edema induced by PMA. It was found that SMLE inhibits the LPS-induced gene and protein expression of iNOS, TNF-α, IL-1β and IL-6 in macrophages by blocking NF-κB activation, and these effects are mediated, at least in part, through the inhibition of intracellular ROS generation and the surface expression of CD14. The results suggest a possible therapeutic application of SMLE in inflammatory diseases and provide scientific evidence in support of the traditional Chinese medical practice of treatment with S. miltiorrhiza.

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Modulation of acute and chronic inflammatory processes by a traditional medicine preparation GCSB-5 both in vitro and in vivo animal models

Cited by 83 publications

References 29 publications

Effect of Hautriwaic Acid Isolated from Dodonaea viscosa in a Model of Kaolin/Carrageenan-Induced Monoarthritis

Effect of Hautriwaic Acid Isolated from Dodonaea viscosa in a Model of Kaolin/Carrageenan-Induced Monoarthritis

Protective effect of GCSB-5, an herbal preparation, against peripheral nerve injury in rats

Lipid‐soluble Extracts from Salvia miltiorrhiza Inhibit Production of LPS‐induced Inflammatory Mediators via NF‐κB Modulation in RAW 264.7 Cells and Perform Antiinflammatory Effects In Vivo

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