Modulation of albumin gene expression by amino acid supply in rat liver is mediated through intracellular concentration of pyridoxal 5′-phosphate

Oka, Tatsuzo; Kuwahata, Masashi; Sugitatsu, Hitomi; Tsuge, Haruhito; Asagi, Kozo; Kohri, Hideaki; Horiuchi, Saburou; Natori, Yasuo

doi:10.1016/s0955-2863(97)00006-5

Cited by 13 publications

(5 citation statements)

References 11 publications

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“…In nutritional terms, this transcription factor is inactivated by directly binding to pyridoxal 5’-phosphate (PLP, the active form of vitamin B6) [ 42 , 43 ]. Furthermore, binding of HNF-1 to the promoter of albumin gene was attenuated and albumin gene expression was suppressed in the liver of rats maintained on an amino acid-depleted parenteral nutrition [ 44 ]. Hepatic PLP level was higher and pyridoxamine 5’-phosphate (PMP) level was lower in amino acid-depleted rats compared with amino acid-infused rats.…”

Section: Nutritional Regulation Of Albumin Metabolismmentioning

confidence: 99%

Potential Role of Amino Acid/Protein Nutrition and Exercise in Serum Albumin Redox State

2017

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Albumin is the major protein in the serum of mammals. It is synthesized exclusively in the liver, before being secreted into the circulation. Similar to skeletal muscle protein, albumin synthesis is stimulated by dietary amino acids and proteins as well as exercise. Albumin has three isoforms based on the redox states of the free cysteine residue at position 34. The redox state of serum albumin has long been extensively investigated in terms of oxidative stress-related chronic diseases, with the redox state of serum albumin having been regarded as a marker of systemic oxidative stress. However, according to recent animal studies, the redox state of serum albumin is modulated by albumin turnover and may also reflect amino acid/protein nutritional status. Furthermore, as the redox state of serum albumin is modulated by exercise training, measuring the pre- and post-exercise redox states of serum albumin in athletes may be useful in assessing amino acid/protein nutritional status and exercise-induced oxidative stress, which are closely associated with skeletal muscle adaptive responses. This article extensively reviews serum albumin and the redox state of albumin in the context of amino acid/protein nutritional status and exercise training.

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Section: Nutritional Regulation Of Albumin Metabolismmentioning

confidence: 99%

Potential Role of Amino Acid/Protein Nutrition and Exercise in Serum Albumin Redox State

2017

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“…The mRNA expression of HNF-1 is downregulated upon administration of macromolecular substances 24 and its binding capacity to the promoter region of the albumin gene is repressed during amino acid deficiency. 25 We found that the mRNA expression of albumin was significantly decreased in the livers of GE + Alb group rats as compared with the Non-surg and AGE groups rats. Moreover, the albumin mRNA expression was found to be lower in the GE and AGE + Alb groups, but the differences were not significant.…”

Section: Discussionmentioning

confidence: 69%

“…Furthermore, HNF‐1 is an important regulator of albumin synthesis, 17,18 as it enhances transcription of the albumin gene by binding to its promoter region. The mRNA expression of HNF‐1 is downregulated upon administration of macromolecular substances 24 and its binding capacity to the promoter region of the albumin gene is repressed during amino acid deficiency 25 . We found that the mRNA expression of albumin was significantly decreased in the livers of GE + Alb group rats as compared with the Non‐surg and AGE groups rats.…”

Section: Discussionmentioning

confidence: 70%

Effects of amino acids and albumin administration on albumin metabolism in surgically stressed rats: A basic nutritional study

Wada

Nakamura

Kobayashi

et al. 2023

J Parenter Enteral Nutr

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Background: Nutrition therapy and administration of albumin preparations are common in postsurgical patients. However, the effects of these interventions on albumin metabolism are unclear. We elucidated the effect of postoperative albumin and/or parenteral nutrition administration on it.Methods: Sprague-Dawley rats underwent surgery involving intestinal rubbing followed by intestinal exposure. Subsequently, they were administered experimental solutions for 48 h, their blood samples were collected at 24 and 48 h, and livers were excised at 48 h. Based on experimental solutions, rats were divided into five groups: non-surgical (Non-surg); glucose and electrolyte solution (GE); amino acid, glucose, and electrolyte solution (AGE); GE + rat serum albumin (Alb) (GE + Alb); and AGE + Alb. Their plasma albumin concentrations; albumin fractional synthesis rate (ALB FSR); mercaptoalbumin/total albumin ratio (MA ratio); and messenger RNA (mRNA) expressions of albumin and hepatocyte nuclear factor-1 (HNF-1) in the liver were measured. Results:The GE and AGE groups showed significant decline in albumin concentrations.ALB FSR was significantly enhanced in the AGE group compared with the GE group.The mRNA expression of albumin was similar to ALB FSR in all groups and that of HNF-1 was significantly decreased in the GE + Alb and AGE + Alb groups compared with the Non-surg group. The MA ratio in the AGE group was similar to the Non-surg group. Conclusion:The administration of amino acids comprising parenteral nutrition after surgery augmented ALB FSR and maintained the MA ratio only without simultaneous albumin administration.

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“…In our recent study (Oka et al 1997), rats were nourished by infusion of total parenteral nutrition solutions containing 0 or 33 g amino acids/kg for 7 d. The level of albumin mRNA in the liver of amino acid-infused rats was found to be several-fold higher than that in the liver of amino acid-depleted rats. Since the expression of the albumin gene is regulated by tissuespecific transcription factors, HNF-1 and C/EBP, we determined the binding activities of nuclear extracts to the HNF-1-and C/EBP-binding sites by mobility-shift analysis and found that the activity of the extract prepared from livers of amino acid-infused rats was greater than that of amino acid-depleted rats.…”

Section: Expression Of the Albumin Gene And Amino Acid And Protein Numentioning

confidence: 94%

Modulation of gene expression by vitamin B6

Oka¹

2001

NRR

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The physiologically active form of vitamin B6, pyridoxal 5'-phosphate (PLP), is known to function as a cofactor in many enzymic reactions in amino acid metabolism. Recent studies have shown that, apart from its role as a coenzyme, PLP acts as a modulator of steroid hormone receptor-mediated gene expression. Specifically, elevation of intracellular PLP leads to a decreased transcriptional response to glucocorticoid hormones, progesterone, androgens, and oestrogens. For example, the induction of cytosolic aspartate aminotransferase (cAspAT) in rat liver by hydrocortisone is suppressed by the administration of pyridoxine. The suppression of the cAspAT induction by pyridoxine is caused by a decrease in the expression of the cAspAT gene, which is brought about by inactivation of the binding activity of the glucocorticoid receptor to the glucocorticoid-responsive element in the regulatory region of the cAspAT gene. Vitamin B6 has recently been found to modulate gene expression not only for steroid hormone-responsive or PLP-dependent enzymes but also for steroid- and PLP-unrelated proteins such as serum albumin. Albumin gene expression was found to be modulated by vitamin B6 through a novel mechanism that involves inactivation of tissue-specific transcription factors, such as HNF-1 or C/EBP, by direct interaction with PLP in a similar manner to glucocorticoid receptor. Enhancement of albumin gene expression in the liver by an increased supply of amino acids can be explained by elevated binding of HNF-1 and C/EBP to their DNA-binding sites which, in turn, is caused by a decrease in the intracellular level of PLP by the increased amino acid supply. These findings that vitamin B6 acts as a physiological modulator of gene expression add a new dimension to the hitherto recognized function of vitamin B6 as a cofactor of enzyme action.

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Modulation of albumin gene expression by amino acid supply in rat liver is mediated through intracellular concentration of pyridoxal 5′-phosphate

Cited by 13 publications

References 11 publications

Potential Role of Amino Acid/Protein Nutrition and Exercise in Serum Albumin Redox State

Potential Role of Amino Acid/Protein Nutrition and Exercise in Serum Albumin Redox State

Effects of amino acids and albumin administration on albumin metabolism in surgically stressed rats: A basic nutritional study

Modulation of gene expression by vitamin B6

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