Modulation of androgen receptor protein by culture conditions of human skin fibroblasts

Abstract

Cultures of skin fibroblasts show variation of androgen binding with culture conditions; binding variations are usually avoided by using confluent cultures. In this work, we analysed the effect of cell density and mitogenic agents on the level of androgen receptor (AR) of cultured human skin fibroblasts. Results demonstrated that in cultures of human skin fibroblasts, cellular binding of dihydrotestosterone was higher in cells grown at low than at high cell density. The reduction in binding resulted from a decrease in the number of high affinity receptors and not from a change in receptor affinity. Immunocytochemistry for AR showed greater staining intensity in cells grown at low than at high cell density. Additionally, immunoblot analysis demonstrated more AR protein in low cell density cultures. On the other hand, it was observed that cells grown at low cell density showed diminished androgen binding capacity after 24 h of treatment with insulin-like growth factor (IGF-l), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), or granulocyte-colony stimulating factor (G-CSF); this effect of growth factors was not observed in cells grown at high cell density. In conclusion, we found that cell density of cultures and mitogenic agents can regulate AR binding activity in human fibroblasts. While we do not yet know how changes in cell density affect the amount of AR, we conclude that the mechanism could be mediated by activation of the tyrosine kinase pathway, as the effect was reproduced by mitogens.

Comparative effect of human andTrypanosoma cruzicalreticulin in wound healing

“…Human skin fibroblasts (kindly provided by Dr M. Fernandez, Institute of Nutrition and Food Technology, University of Chile) were obtained from fresh child preputial skin, as published elsewhere (Palma et al ., ). The dermal fibroblasts were grown in complete Eagle's minimal essential medium (MEM); containing 10% fetal bovine serum (FBS) 2 m m glutamine and antibiotic–antimycotic (ABAM).…”