1998
DOI: 10.1007/s11626-998-0114-x
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Modulation of angiotensin-converting enzyme in cultured human vascular endothelial cells

Abstract: Previous work has suggested that not all immunoreactive angiotensin-converting enzyme (ACE) in tissues or cells is in a biologically active state. We have explored this possibility in cultured human umbilical vein endothelial cells (HUVEC), one of the most widely studied in vitro endothelial cell systems. Our approach included characterization of the effect of increasing passage number on ACE activity and expression of immunoreactive ACE at the single cell level, the subcellular compartmentalization of active … Show more

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Cited by 39 publications
(36 citation statements)
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References 34 publications
(42 reference statements)
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“…We evaluated the effect of Ang II stimulation in an artificial system (CHO), which does not express ACE constitutively, 19,20 transfected with a plasmid containing the sequence of the human somatic enzyme and in Tm5 cells, which express ACE endogenously, but not the AT 1 and AT 2 receptors. We checked Ang receptor expression by different methods (PCR and flow cytometry) and could not detect their presence in CHO nontransfected and mock-transfected cells.…”
Section: Discussionmentioning
confidence: 99%
“…We evaluated the effect of Ang II stimulation in an artificial system (CHO), which does not express ACE constitutively, 19,20 transfected with a plasmid containing the sequence of the human somatic enzyme and in Tm5 cells, which express ACE endogenously, but not the AT 1 and AT 2 receptors. We checked Ang receptor expression by different methods (PCR and flow cytometry) and could not detect their presence in CHO nontransfected and mock-transfected cells.…”
Section: Discussionmentioning
confidence: 99%
“…The resulting peptide, possibly MetLys-BK, is antagonized by a low concentration of the specific B 2 receptor antagonist LF in the umbilical vein. We recently identified ACE in endothelial cells of the human umbilical vein (tissue sections, immunohistochemistry) [11]; in cultured endothelial cells derived from this vessel, the regulated expression of ACE has been described [11,24,25]. As previously reported [15], enalaprilat did not influence the apparent potency of BK in the contractility assay.…”
Section: Discussionmentioning
confidence: 54%
“…As we shall discuss further below, it is important to note that ACE and other components of the RAS are expressed in all the cell types found within the tumor microenvironment, including endothelium, monocytes, macrophages, dendritic cells, fibroblasts, and T cells (11,38,83,125). It is therefore imperative to delineate the possible role and function of ACE in these different compartments to evaluate and maximize pharmacological targeting of the RAS in cancer.…”
Section: The Tumor Microenvironmentmentioning
confidence: 99%