Modulation of antioxidant and detoxification responses mediated by lipoic acid in the fish Corydoras paleatus (Callychthyidae)

Generation of reactive oxygen species in relevant cell lines as a bio-indicator of oxidative effects caused by acid mine water

“…This model system using cell lines that represent the target tissues of toxicity can be routinely implemented as a fast, easy and straightforward manner to estimate the possible consequence of exposure to certain aquatic environmental pollutants involving AMD, because antioxidant competence is a mutually-inclusive measurement in biomonitoring research (Monserrat et al, 2008). A limitation is that there is no standard cut-off level for ROS formation that indicates the degree of safety.…”

“…The Vero cell line was the most sensitive (least antioxidant capacity), as it generated the most ROS across all concentrations in T and in higher concentrations exposed to U. There are reports suggesting differences in vulnerability to oxidative damage (Monserrat et al, 2008;Oliveira et al, 2008). The reason for Vero cell sensitivity is unknown, but the kidneys from which they are derived are important target organs usually related to heavy metal toxicity (Gardner et al, 2006;Barbieri et al, 2009).…”

“…Biomarkers are applied as sensitive indicators of pollution to detect underlying stressful conditions (Stegeman et al, 1992). With regard to pollution, biomarkers of exposure can be assessed based on antioxidant responses and oxidative stress parameters because pollutants enhance intracellular formation of ROS through mechanisms such as redox cycling, or cytochrome P450-dependent metabolism of polycyclic aromatic hydrocarbons and the Fenton reaction in the presence of transition metals (Regoli, 2000;Halliwell and Gutteridge, 2007;Monserrat et al, 2008).…”

“…Studies involving aquatic organisms like fish have reported differences in susceptibility to oxidative damage in organs such as gills, brain, muscle, liver and kidney (Oliveira et al, 2008;Monserrat et al, 2008), some of which was attributed to higher antioxidant basal levels in various tissues. Rather than measuring a limited number of antioxidants or conducting classical oxidative stress studies quantifying antioxidant efficiency individually, an alternative is to evaluate oxidative effects of polluted water on relevant cell lines to serve as a quick and easy screening method when applied together with water quality parameters.…”

Licopeno no bem-estar de juvenis de tilápia do Nilo (Oreochromis niloticus): efeito sobre desempenho e parâmetros bioquímicos

“…Os mesmos efeitos são descritos quando utilizadas substâncias poluentes e tóxicas sobre a atividade de enzimas antioxidantes, em organismos aquáticos, particularmente em peixes. Dentre os poluentes podem ser citados, metais pesados, inseticidas, e dentre as substâncias tóxicas, toxinas, altas doses de antibióticos, entre outros (VINAGRE et al, 2003;PINHO et al, 2005;CAZENAVE et al, 2006;MONSERRAT et al, 2007;FERRARI et al, 2007;PRIETO et al, 2007;OLIVEIRA et al, 2008;MONSERRAT et al, 2008e LEÃO et al, 2008.…”