Modulation of Auxin Levels in Pollen Grains Affects Stamen Development and Anther Dehiscence in Arabidopsis

Salinas-Grenet, Hernán; Herrera-Vásquez, Ariel; Parra, Samuel; Cortez, Allan; Gutiérrez, Lilian K.; Pollmann, Stephan; Gabriel, Leon; Blanco‐Herrera, Francisca

doi:10.3390/ijms19092480

Cited by 21 publications

(19 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Auxin is a morphogen-like compound that plays a pivotal role in pattern formation in plant morphogenesis, 3 including anther development. [6][7][8][9] Auxin is essential for anther initiation, 4 pollen development, anther dehiscence, and other aspects of post-meiotic anther development, [7][8][9]24 but its function in microsporogenesis, i.e. germ cell initiation and specification during pre-meiotic anther development, is unknown.…”

Section: Mainmentioning

confidence: 99%

Auxin guides germ cell specification inArabidopsisanthers

Zheng

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

Germ cells (GCs) transmit genetic information from one generation to the next. Unlike animal GCs, plant GCs are induced post-embryonically, forming locally from somatic cells. This induction is coordinated with organogenesis and might be guided by positional cues. In angiosperms, male GCs initiate from the internal layers at the four corners of the anther primordia and are gradually enclosed by parietal cell (PC) layers, leading to a concentric GC-PC pattern. However, the underlying mechanism of GC initiation and GC-PC pattern formation is unclear. Auxin affects pattern formation3 and anther development. However, whether GC formation involves auxin remains unknown. We report that the auxin distribution in pre-meiotic anthers parallels GC initiation, forming a centripetal gradient between the outer primordial cells and the inner GCs. The auxin biosynthesis genes TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (TAA1) and TRYPTOPHAN AMINOTRANSFERASE RELATED 2 (TAR2) are responsible for this patterning and essential for GC specification. SPOROCYTELESS/NOZZLE (SPL/NZZ, a determinant for GC specification) mediates the effect of auxin on GC specification, modulates auxin homeostasis, and maintains centripetal auxin patterning. Our results reveal that auxin is a key factor guiding GC specification in Arabidopsis anthers.

show abstract

Section: Mainmentioning

confidence: 99%

Auxin guides germ cell specification inArabidopsisanthers

Zheng

Wang

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…In general, the flavin monooxygenase (YUCCA) is involved in the tryptophan-dependent pathway of auxin biosynthesis [26], mutants of YUCCA present aberrant flower phenotypes, short stamen filaments, and thus sterility [27]. Our results show that the expression levels of YUCCA were down-regulated significantly (Table 3), the flower phenotype also appears as short stamen filaments (Fig.…”

Section: Discussionmentioning

confidence: 66%

Transcriptome profiling of the fertile parent and sterile hybrid in tea plant flower buds

Chen

Xia

et al. 2019

Hereditas

View full text Add to dashboard Cite

Background The tea plant is a crucial economic crop. The floral organ development consumes a large amount of nutrients, which affects the leaf yield. To understand the mechanism by which the tea plant produces sterile floral buds, we obtained a sterile tea plant by artificial hybridization. RNA-sequencing based transcriptome analysis was implemented in three samples to determine the differentially expressed genes (DEGs) related to flower development. Results In this study, a total of 1991 DEGs were identified; 1057 genes were up-regulated and 934 genes were down-regulated in sterile hybrid floral buds. These were mainly distributed in the regulation of biological and metabolic processes. Significantly, auxin biosynthesis genes YUCCA , AUX1 and PIN were dramatically down-regulated, and ARF gene was up-regulated in the sterile hybrid floral buds, and flower development-related genes AP1 , AP2 and SPL were changed. A total of 12 energy transfer-related genes were significantly decreased. Furthermore, the expression of 11 transcription factor genes was significantly different. Conclusion The transcriptome analysis suggested that the production of sterile floral buds is a complex bioprocess, and that low auxin-related gene levels result in the formation of sterile floral buds in the tea plant.

show abstract

“…On the other hand, IAA‐Asp is a catabolism conjugate that is not easily hydrolyzed by IAA‐amino acid hydrolases to give free auxin, unlike storage conjugates (IAA‐Ala, IAA‐Leu) (Ludwig‐Muller ). Transgenic Arabidopsis lines expressing genes that convert free IAA into indole‐3‐acetyl‐L‐lysine showed abnormalities in floral organs including abnormal pollen tube growth, perturbations in the synchronization of anther dehiscence and stamen development ultimately resulting in lowering seed set (Salinas‐Grenet et al ).…”

Section: Discussionmentioning

confidence: 99%

Enhanced N‐metabolites, ABA and IAA‐conjugate in anthers instigate heat sensitivity in spring wheat

Bheemanahalli

Impa

Krassovskaya

et al. 2020

Physiologia Plantarum

View full text Add to dashboard Cite

Unraveling the metabolic and phytohormonal changes in anthers exposed to heat stress would help identify mechanisms regulating heat stress tolerance during the sensitive reproductive stage. Two spring wheat genotypes contrasting for heat tolerance were exposed to heat stress during heading in controlled environment chambers. Anthers were collected from main and primary spikes for metabolic and phytohormonal profiling. A significant reduction in seed set (38%), grain number (54%) and grain weight (52%) per plant was recorded in the sensitive (KSG1177) but not in the tolerant (KSG1214) genotype under heat stress compared to control. Anther metabolite accumulation did not vary quantitatively between main and primary spikes. Hierarchical clustering of the genotypes and treatments using metabolites and phytohormones revealed a distinct cluster for KSG1177 under heat stress from that of control and KSG1214. A significant increase in N‐based amino acids, ABA, IAA‐conjugate and a decrease in polyamines and organic acids were observed in wheat anthers exposed to heat stress. Unlike KSG1214, a significantly higher accumulation of amino acids, ABA and IAA‐conjugate in anthers of the sensitive KSG1177 was recorded under heat stress. These findings provide the rationale and direction towards developing molecular markers for enhancing heat stress tolerance in wheat.

show abstract

Modulation of Auxin Levels in Pollen Grains Affects Stamen Development and Anther Dehiscence in Arabidopsis

Cited by 21 publications

References 31 publications

Auxin guides germ cell specification inArabidopsisanthers

Auxin guides germ cell specification inArabidopsisanthers

Transcriptome profiling of the fertile parent and sterile hybrid in tea plant flower buds

Enhanced N‐metabolites, ABA and IAA‐conjugate in anthers instigate heat sensitivity in spring wheat

Contact Info

Product

Resources

About