Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants

Bačáková, Lucie; Filová, Elena; Pařízek, Martin; Ruml, Tomáš; Švorčı́k, Václav

doi:10.1016/j.biotechadv.2011.06.004

Cited by 863 publications

(787 citation statements)

References 216 publications

Supporting

Mentioning

751

Contrasting

Unclassified

Order By: Relevance

“…The extent and the strength of cell adhesion play a decisive role in regulating the subsequent cell proliferation activity. 52 Therefore, fluorescence microscopy and MTS assays were performed to assess cell viability and proliferation on the BC substrates, and on tissue culture polystyrene (TCP) used as a control surface. Metabolic activity was measured after 3, 7 and 14 days ( Figure 7) with MTS assay.…”

Section: Methodsmentioning

confidence: 99%

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

View full text Add to dashboard Cite

The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 μm of average thickness) and heat-dried BC substrates were surface-modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography, and also regarding their permeability, dimensional stability, mechanical properties, and endotoxin content. Then, their ability to promote RPE cell adhesion and proliferation in vitro was assessed. All surface-modified BC substrates presented similar permeation coefficients with solutes of up to 300 kDa. Acetylation of BC decreased it's swelling and the amount of endotoxins. Surface modification of BC greatly enhanced the adhesion and proliferation of RPE cells. All samples showed similar stress-strain behavior; BC and acetylated BC showed the highest elastic modulus, but the latter exhibited a slightly smaller tensile strength and elongation at break as compared to pristine BC. Although similar proliferation rates were observed among the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation. 2 ABSTRACT: The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 µm of average thickness) and heatdried BC substrates were surface modified via acetylation and polysaccharide adsorption using chitosan and carboxymethyl cellulose. The BC substrates were characterized according to surface chemistry, wettability, energy, topography, permeability, dimensional stability, mechanical properties and level of endotoxins present. Then, the ability to promote RPE cell adhesion and proliferation in vitro was assessed. BC substrates were porous and permeable to solutes up to 300 kDa. The acetylation decreased substrate swelling and the amount of endotoxins present. Surface modification greatly enhanced the adhesion and proliferation of RPE cells in BC. Although similar proliferation rates were observed between the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation.

show abstract

Section: Methodsmentioning

confidence: 99%

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

View full text Add to dashboard Cite

show abstract

“…plasma treatments) are sometimes used to increase surface hydrophilicity through the introduction of polar functional groups. In contrast, it is known that the interactions of highly hydrophilic surfaces with ECM adhesion proteins are weak [6]. Keselowsky et al characterized various functional groups on the criteria of fibronectin adsorption (in increasing order): OH < COOH < CH 3 < NH 2 .…”

Section: Surfaces and Bioadhesionmentioning

confidence: 99%

“…The wettability criterion is obviously strongly limited as the surface mechanical properties and structure, cell types and charge density are ignored. For example, Bacakova et al showed that soft matrices are not favorable for cell adhesion [6]. They explained that ECM deposited on such surfaces is not able to resist the forces involved during cell focal point formation.…”

Section: Surfaces and Bioadhesionmentioning

confidence: 99%

See 1 more Smart Citation

PNIPAM grafted surfaces through ATRP and RAFT polymerization: Chemistry and bioadhesion

Conzatti

Cavalié

Combes

et al. 2017

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

a b s t r a c tBiomaterials surface design is critical for the control of materials and biological system interactions. Being regulated by a layer of molecular dimensions, bioadhesion could be effectively tailored by polymer surface grafting. Basically, this surface modification can be controlled by radical polymerization, which is a useful tool for this purpose. The aim of this review is to provide a comprehensive overview of the role of surface characteristics on bioadhesion properties. We place a particular focus on biomaterials functionalized with a brush surface, on presentation of grafting techniques for "grafting to" and "grafting from" strategies and on brush characterization methods. Since atom transfer radical polymerization (ATRP) and reversible addition-fragmentation chain transfer (RAFT) polymerization are the most frequently used grafting techniques, their main characteristics will be explained. Through the example of poly(N-isopropylacrylamide) (PNIPAM) which is a widely used polymer allowing tuneable cell adhesion, smart surfaces involving PNIPAM will be presented with their main modern applications.

show abstract

“…18 Physico-chemical factors also determine and modulate adhesion. 19 If a material is too hydrophobic, ECM molecules are absorbed in a denatured state and the resulting rigid geometric conformation impairs access to integrin binding sites. A polymer surface can be rendered more ''wettable'' (hydrophilic) by physical or chemical means (e.g.…”

Section: Introductionmentioning

confidence: 99%

Modulating patterned adhesion and repulsion of HEK 293 cells on microengineered parylene‐C/SiO₂ substrates

Hughes

Bunting

Cameron

et al. 2012

J Biomedical Materials Res

View full text Add to dashboard Cite

This article describes high resolution patterning of HEK 293 cells on a construct of micropatterned parylene-C and silicon dioxide. Photolithographic patterning of parylene-C on silicon dioxide is an established and consistent process. Activation of patterns by immersion in serum has previously enabled patterning of murine hippocampal neurons and glia, as well as the human hNT cell line. Adapting this protocol we now illustrate high resolution patterning of the HEK 293 cell line. We explore hypotheses that patterning is mediated by transmembrane integrin interactions with differentially absorbed serum proteins, and also by etching the surface substrate with piranha solution. Using rationalized protein activation solutions in place of serum, we show that cell patterning can be modulated or even inverted. These cell-patterning findings assist our wider goal of engineering and interfacing functional neuronal networks via a silicon semiconductor platform.

show abstract

Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants

Cited by 863 publications

References 216 publications

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

PNIPAM grafted surfaces through ATRP and RAFT polymerization: Chemistry and bioadhesion

Modulating patterned adhesion and repulsion of HEK 293 cells on microengineered parylene‐C/SiO₂ substrates

Contact Info

Product

Resources

About

Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants

Cited by 863 publications

References 216 publications

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

PNIPAM grafted surfaces through ATRP and RAFT polymerization: Chemistry and bioadhesion

Modulating patterned adhesion and repulsion of HEK 293 cells on microengineered parylene‐C/SiO2 substrates

Contact Info

Product

Resources

About

Modulating patterned adhesion and repulsion of HEK 293 cells on microengineered parylene‐C/SiO₂ substrates