Modulation of Cell–Cell Interactions in Drosophila Oocyte Development

Antel, Matthew; Inaba, Mayu

doi:10.3390/cells9020274

Cited by 11 publications

(6 citation statements)

References 93 publications

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“…Our analyses suggest that, concomitant with these responses, there are functionally coherent changes in oocyte proteome composition. Although the mechanisms responsible for these differences remain to be elucidated, the stimulation of oocyte maturation may accelerate the temporal progression of oogenesis and this, in turn, may alter the dynamics of protein translation and degradation, as well as the duration of interactions with nurse and follicle cells 49 . Our analysis suggests that mating-induced changes influence proteome dynamics associated with maturation, including transmembrane proteins.…”

Section: Discussionmentioning

confidence: 99%

Drosophila oocyte proteome composition covaries with female mating status

McDonough-Goldstein

Pitnick

Dorus

2021

Sci Rep

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Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. Here, we investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stage-14 oocytes from mated females differed significantly in protein composition relative to oocytes from unmated females. Proteins forming a highly interconnected network enriched for translational machinery and transmembrane proteins were increased in oocytes from mated females, including calcium binding and transport proteins. This mating-induced modulation of oocyte maturation was also significantly associated with proteome changes that are known to be triggered by egg activation. We propose that these compositional changes are likely to have fitness consequences and adaptive implications given the importance of oocyte protein composition, rather than active gene expression, to the maternal-to-zygotic transition and early embryogenesis.

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Section: Discussionmentioning

confidence: 99%

Drosophila oocyte proteome composition covaries with female mating status

McDonough-Goldstein

Pitnick

Dorus

2021

Sci Rep

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“…Although a complex sequence of signaling events and close reciprocal interactions between somatic and germline cells occur during the early Drosophila oogenesis [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ] open questions remain on the structural interplay between the follicle cells and the oocyte. We examined here the morphology of the cell contacts during egg chamber formation and early stages of vitellogenesis to add insights to the general understanding of the reciprocal interaction between somatic and germ cells in Drosophila oogenesis.…”

Section: Introductionmentioning

confidence: 99%

Cell-to-Cell Interactions during Early Drosophila Oogenesis: An Ultrastructural Analysis

Riparbelli

Persico

Callaini

2022

Cells

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Drosophila oogenesis requires the subsequent growth of distinct egg chambers each containing a group of sixteen germline cells surrounded by a simple epithelium of follicle cells. The oocyte occupies a posterior position within the germ cells, thus giving a distinct asymmetry to the egg chamber. Although this disposition is critical for the formation of the anterior–posterior axis of the embryo, the interplay between somatic and germ cells during the early stages of oogenesis remains an open question. We uncover by stage 2, when the egg chambers leaved the germarium, some unique spatial interactions between the posterior follicle cells and the oocyte. These interactions are restricted to the surface of the oocyte over the centriole cluster that formed during early oogenesis. Moreover, the posterior follicle cells in front of the oocyte display a convoluted apical membrane with extensive contacts, whereas the other follicle cells have a flat apical surface without obvious surface protrusions. In addition, the germ cells located at the posterior end of the egg chamber have very elongated protrusions that come into contact with each other or with facing follicle cells. These observations point to distinct polarization events during early oogenesis supporting previous molecular data of an inherent asymmetry between the anterior and the posterior regions of the egg chambers.

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“…This is a departure from the in vivo microenvironment, where cells and tissues interact intricately with an array of adherent ligands within 3D ECM [ 112 ]. Since cell–cell and cell–matrix interactions are critical to the follicle and oocyte development [ 113 ]. 3D follicular culture systems have shown better rates of follicular survival and growth than their 2D counterparts, while preserving follicular morphology and reducing oocyte maturation gene expression to better mimic in vivo conditions.…”

Section: Ovarian Research Using Microfluidic Systemsmentioning

confidence: 99%

Revolutionizing the female reproductive system research using microfluidic chip platform

Yan,

Wu,

Zhang

et al. 2023

J Nanobiotechnol

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Comprehensively understanding the female reproductive system is crucial for safeguarding fertility and preventing diseases concerning women's health. With the capacity to simulate the intricate physio- and patho-conditions, and provide diagnostic platforms, microfluidic chips have fundamentally transformed the knowledge and management of female reproductive health, which will ultimately promote the development of more effective assisted reproductive technologies, treatments, and drug screening approaches. This review elucidates diverse microfluidic systems in mimicking the ovary, fallopian tube, uterus, placenta and cervix, and we delve into the culture of follicles and oocytes, gametes’ manipulation, cryopreservation, and permeability especially. We investigate the role of microfluidics in endometriosis and hysteromyoma, and explore their applications in ovarian cancer, endometrial cancer and cervical cancer. At last, the current status of assisted reproductive technology and integrated microfluidic devices are introduced briefly. Through delineating the multifarious advantages and challenges of the microfluidic technology, we chart a definitive course for future research in the woman health field. As the microfluidic technology continues to evolve and advance, it holds great promise for revolutionizing the diagnosis and treatment of female reproductive health issues, thus propelling us into a future where we can ultimately optimize the overall wellbeing and health of women everywhere. Graphical Abstract

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Modulation of Cell–Cell Interactions in Drosophila Oocyte Development

Cited by 11 publications

References 93 publications

Drosophila oocyte proteome composition covaries with female mating status

Drosophila oocyte proteome composition covaries with female mating status

Cell-to-Cell Interactions during Early Drosophila Oogenesis: An Ultrastructural Analysis

Revolutionizing the female reproductive system research using microfluidic chip platform

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