Modulation of cell surface expression of liver carbohydrate receptors during in vivo induction of apoptosis with lead nitrate

Ruzittu, Maria Teresa; Carlà, E. C.; Montinari, Maria Rosa; Maietta, Gennaro; Dini, Luciana

doi:10.1007/s004419900059

Cited by 21 publications

(15 citation statements)

References 32 publications

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“…It is worth noting that endothelial liver cells show the highest resistance to in vivo Pb(NO 3 ) 2 injection, whereas Kupffer cells undergo apoptosis within 24 hours from the injection [27]. It has been increasingly recognized that, under normal and pathological conditions, many hepatocyte functions are regulated by substances released by neighbouring non-parenchymal cells [28]. In fact, many mediators performing multiple paracrine and autocrine actions are secreted into the blood flow or the intercellular spaces.…”

Section: Discussionmentioning

confidence: 99%

Kupffer cells promote lead nitrate-induced hepatocyte apoptosis via oxidative stress

Pagliara¹,

Carlà²,

Caforio³

et al. 2003

Comp Hepatol

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Background: Apoptosis and its modulation are crucial factors for the maintenance of liver health, allowing hepatocytes to die without provoking a potential harmful inflammatory response through a tightly controlled and regulated process. Since Kupffer cells play a key role in the maintenance of liver function, the aim of this study was to verify whether Kupffer cells are involved in the induction of liver apoptosis after i.v. injection of Pb(NO 3 ) 2 likely by secretion mechanisms.

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Section: Discussionmentioning

confidence: 99%

Kupffer cells promote lead nitrate-induced hepatocyte apoptosis via oxidative stress

Pagliara¹,

Carlà²,

Caforio³

et al. 2003

Comp Hepatol

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“…The mechanisms of apoptotic cell recognition and clearance are complex and still incompletely understood (12). The PS exposed on the surface of apoptotic cells may be an important ligand for specific receptor-mediated phagocytosis (23), but several other changes on the surface of apoptotic cells including changes in cell surface carbohydrates with increased mannose expression may participate (24). Two recent reports support an opsonic role for the collectins in the clearance of apoptotic cells by macrophages.…”

Section: Discussionmentioning

confidence: 99%

Surfactant Protein D Reduces Alveolar Macrophage Apoptosis In Vivo

Clark

Palaniyar

Strong

et al. 2002

The Journal of Immunology

144

127

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Surfactant protein D (SP-D) is a molecule of the innate immune system that recognizes the patterns of surface carbohydrate on pathogens and targets them for phagocytosis and killing. SP-D-deficient mice show an increased number of macrophages in the alveolar space, excess surfactant phospholipid, overproduction of reactive oxygen species, and the development of emphysema. We report here that SP-D-deficient mice have a 5- to 10-fold increase in the number of apoptotic and necrotic alveolar macrophages, as defined by annexin V and propidium iodine staining, respectively. Intrapulmonary administration of a truncated 60-kDa fragment of human recombinant SP-D reduces the number of apoptotic and necrotic alveolar macrophages and partially corrects the lipid accumulation in SP-D-deficient mice. The same SP-D fragment binds preferentially to apoptotic and necrotic alveolar macrophages in vitro, suggesting that SP-D contributes to immune homeostasis in the lung by recognizing and promoting removal of necrotic and apoptotic cells.

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“…3, 4). The peak of phagocytic activity of Kupffer cells (3-fold the control) was measured at 5 and 15 days from lead nitrate injection (Ruzittu et al, 1999;Dini, 2000), thus confirming the capacity (in particular for the interaction with particulate materials) of the hepatic sinusoidal wall to operate as a protective barrier for the systemic circulation (Steffan et al, 1986;Wardle, 1987;Dini and Kolb-Bachofen, 1989;KolbBachofen, 1992;Toth and Thomas, 1992).…”

Section: In Vivo Studiesmentioning

confidence: 99%

Phagocytosis of apoptotic cells by liver: A morphological study

Dini

Pagliara

Carlà

2002

Microscopy Res & Technique

123

106

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The present review deals with the morphological features of the removal of apoptotic cells by liver. The engulfment of cells undergoing apoptosis can be considered a specialized form of phagocytosis, playing a major role in the general tissue homeostasis in physiological and pathological conditions. In fact, defects of phagocytosis of apoptotic cells might have deleterious consequences for neighboring healthy cells, i.e., pathogenesis of inflammatory disease or dysregulation of the immune system. Phagocytosis of apoptotic cells by liver is a complex phenomenon, involving multiple molecular mechanisms of recognition (i.e., lectin-like receptors and receptors for externalized phosphatydilserine) of both parenchymal (hepatocytes) and nonparenchymal (Kupffer and endothelial cells) liver cells, often operating in cooperation. The data discussed in the present review are drawn from studies of phagocytosis of apoptotic cells in the liver, carried out with in vivo and in situ adhesion experiments as well as in vitro assays. Our results indicate that the three main liver cell types (hepatocytes, Kupffer, and endothelial cells) are able to recognize and internalize apoptotic cells by means of specific receptors (galactose and mannose-specific receptor; receptor for phosphatydilserine) and by cytoskeletal reorganization that favors the engulfment of the apoptotic cells. The "flags" for the identification of apoptotic cells by the liver are modifications of the surface of dead cells, i.e., sugar residues and phosphatydilserine exposition. Vitronectin receptor is not involved in such a recognition. The adhesions between modified cell surfaces of apoptotic cells and phagocytes generate cytoplasmatic signaling pathways that drive apoptotic cells to their final fate within the phagocytes (i.e., lysosomal digestion).

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Modulation of cell surface expression of liver carbohydrate receptors during in vivo induction of apoptosis with lead nitrate

Cited by 21 publications

References 32 publications

Kupffer cells promote lead nitrate-induced hepatocyte apoptosis via oxidative stress

Kupffer cells promote lead nitrate-induced hepatocyte apoptosis via oxidative stress

Surfactant Protein D Reduces Alveolar Macrophage Apoptosis In Vivo

Phagocytosis of apoptotic cells by liver: A morphological study

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