Modulation of Cellular Response to Arsenic Trioxide Toxicity by Resveratrol

Abstract

Arsenic trioxide (As2O3) is an environmental carcinogen and a putative endocrine disruptor. Resveratrol has been shown to reverse As2O3-induced oxidative damage. In immortalized but nontransformed estrogen receptor α-negative human breast cells (MCF10A), we observed that 25 μM resveratrol ameliorated As2O3-induced cytotoxicity. As2O3, in the presence or absence of 25 μM resveratrol, induced quinone reductase (NAD(P)H quinone dehydrogenase 1), via the induction of NFE2-related factor 2. As2O3 caused a repression of cytochrome P450 (CYP)1B1, but the addition of 25 μM resveratrol rescued the expression of cytochrome P450 1B1 and kept it at a constant level. Therefore, 25 μM resveratrol can modulate the effects of As2O3 on enzymes involved in estrogen metabolism.

Assessment of Autophagy as Possible Mechanism of the Antitumor Effects of Arsenic Trioxide and/or Cisplatin on Ehrlich Ascites Carcinoma Model

“…Our data indicated that, ATO side effects are endurable. These results are agreed with Mondal et al,(2018).Treatment of EAC mice with ATO (GP6) , CIS (GP7) and combination between ATO & CIS (GP8) resulted in a significant decrease in body weight gain when compared with non-treated EAC mice (GP2) that because ATO induced apoptosis so tumor volume decreased so body weight decreased in the previous groups. These result agreed with Zhao et al,(2014).…”

Estrogen genotoxicity causes preferential development of Fuchs endothelial corneal dystrophy in females

“…The lyophilized residue was resuspended in 70 μl of methanol/water 1:1 with 0.1 % formic acid and filtered through a 5000-molecular weight cutoff filter (Millipore) before analysis by ultraperformance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS). The details of sample analysis on UPLC/MS/MS were described in earlier studies [ 7 , 44 ]. The levels of metabolites in the mouse samples were normalized to the weight of the corneal cups, while those in the human specimens were normalized to the DNA concentration determined from the pellet using QIAamp DNA mini kit (#51304, Qiagen, USA) and a PicoGreen kit (#P7589, Thermo Fisher, USA) for DNA extraction and quantification respectively.…”

Effects of combination treatment using arsenic trioxide and resveratrol on human breast cancer (MCF-7) cells

“…In addition, RSV was shown to be safe in healthy volunteers in one clinical trial [19]. Furthermore, RSV has been found to inhibit the proliferation and promote the apoptosis of tumor cells induced by ATO and protect healthy cells against the toxicity wrought by ATO [20][21][22][23].…”