Modulation of chemotaxis, O2− production and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid of Flavobacterium

Kawai, Yohko

doi:10.1016/s0928-8244(00)00157-7

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…However, lipids containing lysine or serine amino acids, or serine-glycine dipeptides, with analogous backbone structures to OL have previously been observed in Agrobacterium tumefaciens [18] and Flavobacterium meningosepticum [19]. Unfortunately, little detail regarding the molecular structures of OL in R. sphaeroides is available, with prior work limited to their identification based on their migration position on TLC plates using ninhydrin (amino-positive) and molybdate (phosphate-negative) spray results [6,16,17].…”

Section: Introductionmentioning

confidence: 98%

Characterization of ornithine and glutamine lipids extracted from cell membranes of Rhodobacter sphaeroides

Zhang

Ferguson‐Miller

Reid

2009

J. Am. Soc. Mass Spectrom.

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The identification and structural characterization of a series of ornithine lipids extracted from the cell membranes of wild-type Rhodobacter sphaeroides, as well as from a glycerophosphocholinedeficient strain, have been achieved by multistage tandem mass spectrometry of their protonated and deprotonated precursor ions in a linear quadrupole ion trap. Systematic examination of the multistage gas-phase fragmentation reactions of these ions, combined with the use of hydrogen/deuterium exchange, has enabled the pathways responsible for sequential losses of the 3-hydroxy linked fatty acyl chain and the amide linked 3-OH fatty acyl chain from these lipids, as well as for formation of the previously reported ornithine specific positively charged "fingerprint" ion at m/z 115, to be determined. Additionally, the fragmentation pathways responsible for formation of a previously unreported ornithine lipid head group-specific product ion at m/z 131 in negative ion mode have been examined. Based on these results, and by comparison with the fragmentation behavior of model lipoamino acid standard compounds, a series of novel glutamine containing lipids have also been identified, with analogous structures but with masses 14 Da higher than those of several of the ornithine lipids observed in this study. Characteristic "fingerprint" ions indicative of these glutamine lipids were found at m/z 147, 130, and 129 in positive ion mode and at m/z 145 and 127 in negative ion mode. The results from this study establish an experimental basis for future efforts aimed at the sensitive identification, characterization, and quantitative analysis of ornithine and glutamine lipids in complex unfractionated cellular extracts. (J Am Soc Mass Spectrom 2009, 20, 198 -212)

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Section: Introductionmentioning

confidence: 98%

Characterization of ornithine and glutamine lipids extracted from cell membranes of Rhodobacter sphaeroides

Zhang

Ferguson‐Miller

Reid

2009

J. Am. Soc. Mass Spectrom.

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“…Several mechanisms that protect Flavobacterium spp. from ROS, such as superoxide dismutase (SOD) and catalase activity have been described previously (Sanchez-Moreno et al 1989, Kawai et al 2000, Nematollahi et al 2003. The presence or enhanced expression of one of these mechanisms in the highly virulent strain may account for higher resistance to killing by ROS.…”

Section: Discussionmentioning

confidence: 98%

Early interactions of Flavobacterium psychrophilum with macrophages of rainbow trout Oncorhynchus mykiss

Nematollahi¹,

Pasmans²,

Haesebrouck³

et al. 2005

Dis. Aquat. Org.

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The early interactions of a low and a highly virulent Flavobacterium psychrophilum strain with head kidney and spleen macrophages of rainbow trout Oncorhynchus mykiss were characterized. The highly virulent strain was killed 5.8 to 11 times less frequently than the low virulent strain. The head kidney macrophages showed a microbicidal activity approximately twice as high as that of the spleen macrophages. A 2-to 3-fold higher production of reactive oxygen species (ROS) was induced by the highly virulent strain than by the low virulent one. The head kidney macrophages produced approximately twice as much ROS as the spleen macrophages. The low virulent strain was killed approximately 10 times more frequently by H 2 O 2 than was the highly virulent strain. In spleen macrophages, the highly virulent strain caused twice as much cytotoxic effects compared to the low virulent strain. In conclusion, virulence in F. psychrophilum appears to be correlated with higher O. mykiss macrophage cytotoxicity and resistance to ROS and, therefore, with enhanced resistance to bacterial killing. Moreover, due to lower ROS production, spleen macrophages have a lower antimicrobial action against F. psychrophilum, compared to head kidney macrophages and, thus, might form a 'safe site' in which bacteria can reside.KEY WORDS: Rainbow trout · Flavobacterium psychrophilum · Macrophage · Cytotoxic · Reactive oxygen species Resale or republication not permitted without written consent of the publisherDis Aquat Org 64: [23][24][25][26][27][28] 2005 philum bacteria, but only on the highly virulent strain and in the course of time (Decostere et al. 2001). Based on these data, one might speculate that F. psychrophilum virulence is associated with interactions with the host macrophages. The present study aims at deciphering in greater depth the association between bacterial virulence and early interactions of F. psychrophilum with rainbow trout macrophages. MATERIALS AND METHODSFish. Thirty rainbow trout Oncorhynchus mykiss weighing between 500 and 800 g were obtained from a commercial fish farm (Dilbeek, Belgium), where Flavobacterium psychrophilum infections had never before been diagnosed. The fish were kept in a flowthrough system (2000 l tank) containing aerated well water (12 to 14°C) for 3 wk prior to experimentation (pH 7.6; total hardness 100 ppm; NH 3 < 0.01 ppm; NO 2 < 0.01 ppm; dissolved oxygen 9 ppm). The fish were fed daily with a commercial diet (Trouvit Perle Response 2000, Trouw). All fish were evaluated clinically before inclusion in the experiments as described below. Samples were taken from the skin, fins and gills for evaluation of external parasitic infestation. Wet mount preparations were then made and examined microscopically. The fish were clinically healthy and found to be free of external parasite infestations. The presence of F. psychrophilum was assessed by streaking swabs from the skin and gills onto Shieh agar (Shieh 1980). Plates were incubated at 17°C for 5 d. F. psychrophilum was not isolated from an...

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“…We have previously demonstrated that neutrophilic enzymatic activity is enhanced by stimulation with FMLP and proinflammatory cytokines. MPO was believed to exist not on the surface but inside neutrophils and seemed to leak MPO and other lysosomal peroxidase from the granules when neutrophils were activated (24). However, no direct evidence was observed to leak MPO on the neutrophil surface during neutrophil activation, whereas expression of PR3 on the surface of the primed neutrophils can be detected (4,13,50).…”

Section: Discussionmentioning

confidence: 99%

Trafficking of QD‐Conjugated MPO‐ANCA in Murine Systemic Vasculitis and Glomerulonephritis Model Mice

Hoshino

Nagao

Ito‐Ihara

et al. 2007

Microbiology and Immunology

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In systemic vasculitis, the serum level of myeloperoxidase (MPO)‐specific anti‐neutrophil cytoplasmic autoantibodies (MPO‐ANCA) is significantly elevated with the progression of disease. We have established a model of murine systemic vasculitis by administration of MPO‐ANCA and fungal mannoprotein to C57BL/6 mice. We examined the role of MPO and MPO‐ANCA in the pathogenesis of glomerulonephritis and systemic vasculitis in this model using quantum dots (QDs). We demonstrated that QD‐conjugated MPO‐ANCA (ANCA‐QD) visualized the translocation of MPO on the neutrophil membrane surface after stimulation with proinflammatory cytokines. We also observed that MPO translocation on neutrophils in both patients with rapid progressive glomerulonephritis and these model mice without any stimulation, suggesting that MPO translocation is certain to contribute to the development of glomerular lesion. In addition, blood flow on the kidney surface vessel was significantly decelerated in both SCG/Kj mice and this model, suggesting that ANCA induces the damage of blood vessel. These results indicate that MPO‐ANCA and surface‐translocated MPO on the activated neutrophils coordinately plays essential roles in the initial steps of the glomerulonephritis.

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Modulation of chemotaxis, O2− production and myeloperoxidase release from human polymorphonuclear leukocytes by the ornithine-containing lipid and the serineglycine-containing lipid of Flavobacterium

Cited by 9 publications

References 15 publications

Characterization of ornithine and glutamine lipids extracted from cell membranes of Rhodobacter sphaeroides

Characterization of ornithine and glutamine lipids extracted from cell membranes of Rhodobacter sphaeroides

Early interactions of Flavobacterium psychrophilum with macrophages of rainbow trout Oncorhynchus mykiss

Trafficking of QD‐Conjugated MPO‐ANCA in Murine Systemic Vasculitis and Glomerulonephritis Model Mice

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