Modulation of cholinergic neurotransmission by vasoactive intestinal peptide in ferret trachea

Sekizawa, Kiyohisa; Tamaoki, Jun; Pd, Graf; Ja, Nadel

doi:10.1152/jappl.1988.64.6.2433

Cited by 28 publications

(16 citation statements)

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“…However, in the dog trachea neither antagonist produced any effect on the amplitude of the ej.p. or the contractions evoked by field stimulation at concentrations ranging from 10-9 to 10-8 M. It has been reported that low concentrations of exogenous VIP (10-l 10-9 M) act prejunctionally to inhibit ACh release from the vagus nerves in the dog, cat and ferret trachea (Sekizawa et al, 1988). Furthermore in the present experiments, VIP antagonists blocked the prejunctional action of exogenous VIP to suppress the ej.p.…”

Section: Methodssupporting

confidence: 62%

Effects of vasoactive intestinal polypeptide antagonists on cholinergic neurotransmission in dog and cat trachea

Xie

Hirose

Hakoda

et al. 1991

British J Pharmacology

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investigated by use of microelectrode, double sucrose-gap and tension recording methods. 2 In the dog trachea, repetitive stimuli at high frequency (20 Hz) markedly enhanced the amplitude of contraction, the amplitude of contractions evoked by 50 stimuli at 20Hz relative to that evoked by 5 stimuli being 14.2 + 3.8 times (n = 7, ± s.d.). In the cat, the summation was much less marked, the amplitude of contractions evoked by 50 stimuli relative to that evoked by 5 stimuli being only 2.1 + 0.6 times (n = 5, + s.d.). Neither VIP antagonist had any effect on the relationship between the number of stimuli at 20Hz and the relative amplitude of contraction in the dog trachea, but did enhance the amplitude of contractions to 1.1-1.5 times control in the cat trachea. 3 VIP antagonists dose-dependently enhanced the amplitude of excitatory junction potentials (ej.ps) evoked by a single stimulus in the cat trachea, without changing the resting membrane potential or input membrane resistance of the smooth muscle cells. However, neither antagonist had any effect on the amplitude of the ej.p. in the dog trachea. 4 Neither VIP antagonist had any effect on the post-junctional response of smooth muscle cells to exogenously applied acetylcholine (ACh; 10-9-10-s M) in the dog or cat trachea.

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Section: Methodssupporting

confidence: 62%

Effects of vasoactive intestinal polypeptide antagonists on cholinergic neurotransmission in dog and cat trachea

Xie

Hirose

Hakoda

et al. 1991

British J Pharmacology

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“…VIP has been shown to inhibit prejunctionally cholinergic contractions in the airway (Sekizawa et al, 1988: Aikawa et al, 1990Hakoda & Ito, 1990;Xie et al, 1991). However, a-chymotrypsin which inhibits the relaxant action of VIP in the airway (Li & Rand, 1991) did not alter methylene blue-induced potentiating responses to EFS, suggesting that prejunctional inhibition of cholinergic contractions is not mediated via VIP in rat trachea.…”

Section: Discussionmentioning

confidence: 94%

“…In the airway, NO plays a role in NANC relaxation of tracheal smooth muscle in guinea-pigs (Tucker et al, 1990;Li & Rand, 1991). Vasoactive intestinal polypeptide (VIP), another candidate for NANC inhibitory neurotransmitters, has been shown to inhibit cholinergic neurotransmission in ferret (Sekizawa et al, 1988), cat (Aikawa et al, 1990;Hakoda & Ito, 1990; Xie et al, 1991) and dog (Hakoda & Ito, 1990;Xie et al, 1991) trachea as well as relaxing airway smooth muscle (Altiere & Diamond, 1984;Palmer et al, 1986). However, the modulatory effect of NO on cholinergic neurotransmission is not known.…”

Section: Introduction Methodsmentioning

confidence: 99%

The role of nitric oxide in cholinergic neurotransmission in rat trachea

Sekizawa

Fukushima

Ikarashi

et al. 1993

British J Pharmacology

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1 We have investigated the role of nitric oxide (NO) in cholinergic contraction in rat trachea.2 Methylene blue (1O nM to 30 fLM) potentiated cholinergic contraction induced by electrical field stimulation (EFS) at 5 Hz in a concentration-dependent fashion. At a concentration of 30 i1M, methylene blue decreased responses to log EFS frequency, producing 50% of maximum contraction from a control value of 0.74 ± 0.09 Hz to 0.30 ± 0.05 Hz without a significant effect on concentration-response curves to acetylcholine (ACh).3 NG-monomethyl-L-arginine (L-NMMA; 100 1AM) also potentiated cholinergic contraction induced by EFS at 5 Hz (131.5 ± 4.6% of control) without having any effect against ACh (3 jiM)-induced contractions. Likewise, L-NMMA (10011M) significantly increased EFS (5 Hz)-evoked release of ACh from tracheal segments into the bath solution (51.4 ± 4.0 pmol ml-' in the presence of L-NMMA and 35.0 ± 1.8 pmol ml-' in the absence of L-NMMA, respectively).4 Administration of NO (present in acidified soluton of NaNO2) (1 nM to 10 tM) and sodium nitroprusside (100 nM to 10 ytM) concentration-dependently reduced EFS (5 Hz)-induced cholinergic contractions without having a significant effect on ACh (3 tLM)-induced contractions. These results were unaffected by prior exposure of the tissues to L-NMMA (100 ftM). 7 These results suggest that an endogenous NO-like factor may mediate prejunctional inhibition of cholinergic contraction through a cyclic GMP-dependent mechanism in rat trachea.

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“…It is well known that VIP relaxes the airway smooth muscle [23], In addition, at a concentration of VIP less than that needed to relax airway smooth muscle, this agent prejunctionally reduces the release of ACh from the vagus nerve terminal in ferret, feline [5][6][7] and guinea pig airways [24,25], VIP and ACh coexist in the same nerves in the feline trachea [2][3][4], and VIP may be released as a cotransmitter. VIP release may protect against bronchoconstriction by both direct and indirect mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…3], Ultrastructural studies have demonstrated the presence of peptidergic granules within the vagus nerve in the airway smooth muscle [4], VIP and acetylcholine (ACh) may coexist in the same nerves and be released simultaneously. It was recently demonstrated that a concentration of VIP below that needed to relax airway smooth muscle reduced the release of ACh from the vagus nerve terminal in the airways of the ferret and cat [5][6][7].…”

mentioning

confidence: 99%

Pre- and Post-Junctional Effects of VIP-Like Peptides in Guinea Pig Tracheal Smooth Muscle

et al. 1997

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To determine the role of VIP-like peptides on neurotransmission of vagus nerve, we evaluated the effects of helodermin, helospectin, and vasoactive intestinal peptide (VIP) on the contraction of guinea pig tracheal smooth muscle evoked by electrical field stimulation (EFS) or the exogenous application of acetylcholine (ACh). Isometric tension of tracheal strips was measured in the presence of indomethacin (10^-6M) and of guanethidine (10^-6M). VIP (10^-9M to 10^-1M) significantly suppressed the contraction evoked by EFS. VIP, at concentrations of 10^-9 M and 10^-8M, did not affect the ACh-evoked contraction, but a concentration of 10^-7M suppressed ACh-evoked contraction. Helospectin and helodermin (10^-8M and 10^-7M) significantly suppressed the EFS-evoked contraction, but 10^-9M showed no effect. Helospectin and helodermin had no effect on the ACh sensitivity of smooth muscle up to 1(10^-8M, but a concentration of 10^-7M suppressed the ACh-evoked contraction. These results indicate that helodermin, helospectin, and VIP exert both pre- and post-junctional inhibitory effects on the airway smooth muscle of guinea pigs. These peptides, thus, inhibited tracheal smooth muscle contraction prejunctionally at low concentrations, and acted postjunctionally at higher concentrations.

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Modulation of cholinergic neurotransmission by vasoactive intestinal peptide in ferret trachea

Cited by 28 publications

References 0 publications

Effects of vasoactive intestinal polypeptide antagonists on cholinergic neurotransmission in dog and cat trachea

Effects of vasoactive intestinal polypeptide antagonists on cholinergic neurotransmission in dog and cat trachea

The role of nitric oxide in cholinergic neurotransmission in rat trachea

Pre- and Post-Junctional Effects of VIP-Like Peptides in Guinea Pig Tracheal Smooth Muscle

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