2020
DOI: 10.3389/fmicb.2020.01986
|View full text |Cite
|
Sign up to set email alerts
|

Modulation of Cytokines and Extracellular Matrix Proteins Expression by Leishmania amazonensis in Susceptible and Resistant Mice

Abstract: Leishmaniases are a complex of diseases with a broad spectrum of clinical forms, which depend on the parasite species, immunological status, and genetic background of the host. In the Leishmania major model, susceptibility is associated with the Th2 pattern of cytokines production, while resistance is associated with Th1 response. However, the same dichotomy does not occur in L. amazonensis -infected mice. Cytokines are key players in these diseases progression, wh… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

0
18
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
7

Relationship

2
5

Authors

Journals

citations
Cited by 14 publications
(18 citation statements)
references
References 106 publications
0
18
0
Order By: Relevance
“…In addition to cellular and lipid metabolism, infection with Leishmania may lead to modifications of the extracellular matrix (ECM) and collagen composition of the dermis at the site of infection ( 70 , 100 ). In addition, cutaneous leishmaniasis is also characterized by vascular remodeling and lymphangiogenesis mediated by the vascular endothelial growth factor A (VEGF-A)/VEGF receptor 2 (VEGFR-2) signaling pathway that is essential for lesion resolution ( 71 , 101 , 102 ).…”
Section: Discussionmentioning
confidence: 99%
“…In addition to cellular and lipid metabolism, infection with Leishmania may lead to modifications of the extracellular matrix (ECM) and collagen composition of the dermis at the site of infection ( 70 , 100 ). In addition, cutaneous leishmaniasis is also characterized by vascular remodeling and lymphangiogenesis mediated by the vascular endothelial growth factor A (VEGF-A)/VEGF receptor 2 (VEGFR-2) signaling pathway that is essential for lesion resolution ( 71 , 101 , 102 ).…”
Section: Discussionmentioning
confidence: 99%
“…It is known that L. amazonensis infection modulates an organ-compartmentalized cytokine response in susceptible and resistant mice, and Leishmania parasite elimination is accomplished with a robust pro-inflammatory response, accompanied by the release of host-protective cytokines . The upregulation of cytokines that play a part in the inflammatory response in leishmaniasis observed in BALB/c peritoneal macrophages infected or not with L.…”
Section: Discussionmentioning
confidence: 99%
“…Besides inducing iNOS mRNA expression, compound 4 also upregulated the expression of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-12 as well as the regulatory cytokine IL-10. It is known that L. amazonensis infection modulates an organ-compartmentalized cytokine response in susceptible and resistant mice, 50 and Leishmania parasite elimination is accomplished with a robust pro-inflammatory response, accompanied by the release of host-protective cytokines. 51 The upregulation of cytokines that play a part in the inflammatory response in leishmaniasis observed in BALB/c peritoneal macrophages infected or not with L. amazonensis highlighted the in vitro immunomodulatory activity of compound 4 and its potential to enhance the immune response against Leishmania infection.…”
Section: ■ Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Then, qPCR was performed on QuantStudio 3 equipment (Applied Biosystems). The sequences of the specific primers targeting mouse genes were Nfe2l2 - nuclear factor, erythroid derived 2, like 2, transcript variant 1 (Nrf2) (forward 5’TCACACGAGATGAGCTTAGGGCAA3’, reverse 5’TACAGTTCTGGGCGGCGACTTTAT3’), Hmox1 - heme oxygenase 1 (HO-1) (forward 5’CCCAAAACTGGCCTGTAAAA 3’, reverse 5’CGTGGTCAGTCAACATGGAT3’), L-ferritin (forward 5’TTCCAGGATGTGCAGAAGCC3’, reverse 5’AAGAGGGCCTGATTCAGGTTC3’) and Actb - actin, beta (β-actin) (forward 5’AGCTGCGTTTTACACCCTTT3’, reverse 5’AAGCCATGCCAATGTTGTCT3’) ( Tomiotto-Pellissier et al., 2018 ), as well as Nos2 - nitric oxide synthase 2 , inducible, transcript variant 1 (iNOS) (foward 5’GGATCTTCCCAGGCAACCA3’, reverse 5’CAATCCACAACTCGCTCCAA3’) and Rplp0 - ribosomal protein, large, P0 (Rplp0) (foward 5’GCCAGCTCAGAACACTGGTCTA3’, reverse 5’ATGCCCAAAGCCTGGAAGA3’) ( Cardoso et al., 2020 ). The reaction mixtures contained 10 µl of GoTaq ® qPCR Master Mix (Promega), 20 ng of cDNA for Nrf2, HO-1 and β-actina targets, and 100 ng of cDNA for Rplp0 and iNOS targets; 100 nM of Nrf2, HO-1, ferritin and β-actin primers, 600 nM of iNOS primer and 900 nM of Rplp0 primer in a final volume of 20 µl.…”
Section: Methodsmentioning
confidence: 99%