Modulation of dietary fat on the toxicological effects in thymus and spleen in BALB/c mice exposed to perfluorooctane sulfonate

Wang, Yu; Wang, Ling; Liang, Yong; Qiu, Wenhong; Zhang, Jie; Zhou, Qunfang; Jiang, Guibin

doi:10.1016/j.toxlet.2011.04.029

Cited by 26 publications

(21 citation statements)

References 34 publications

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“…Compared with untreated mice, the relative weights of splenic and thymic tissues were not significantly decreased in mice exposed to PFOS. In contrast, Wang et al (2011) conducted a study to explore the underlying mechanism of the immune organ atropy in mice that were fed a regular or high-fat diet and then exposed to PFOS (0, 5, and 20 mg/kg/day) for 14 days. Their results showed that body weight significantly decreased and the immune organs showed considerable atrophy in either the regular or high-fat diet group.…”

Section: Discussionmentioning

confidence: 99%

“…Reports concerning the immunotoxicity of PFOS in vitro and in vivo have appeared in the literature (Keil et al, 2008;Lefebvre et al, 2008;Peden-Adams et al, 2008;Dong et al, 2009;Qazi et al, 2009;Brieger et al, 2011;Mollenhauer et al, 2011;Wang et al, 2011;Zheng et al, 2011;Corsini et al, 2012;Dewitt et al, 2012). However, there is still a lack of understanding of the toxicokinetics and mode(s)/mechanism(s) of immunotoxic action of PFOS.…”

Section: Introductionmentioning

confidence: 99%

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Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Zhang

Wang

Dong

et al. 2012

Journal of Immunotoxicology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Zhang

Wang

Dong

et al. 2012

Journal of Immunotoxicology

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“…A study employing 5e40 mg/kg/day exposure treatment showed that PFOS could induce splenic atrophy and lymphocyte disorders [19]. Studies using a similar range of exposure dosage (5e20 mg/kg/day) for 7e14 days or a 10-day treatment with dietary doses (1%e 0.001%, PFOS w/w) revealed the destructive effect of PFOS on splenic structure, changes in inflammatory response, and cytokine production [20,28,33]. Therefore, we used up to 2.5e10 mg/kg/day as the treatment to simulate the high occupational exposure scenario.…”

Section: Discussionmentioning

confidence: 99%

“…Its dysfunction is usually associated with diminished responsiveness to antigens and increased susceptibility to infection [31]. The bioaccumulation of PFOS in the spleen has been previously reported in rodents [17,32], and several studies have shown that PFOS-induced immunologic outcomes in the spleen involve reduction in weight, structural damages, change in cellular composition, and inflammation [19,33]. To date, information on the underlying mechanisms of these effects is limited.…”

Section: Introductionmentioning

confidence: 99%

“…The microarray technique has the advantages of taking a snapshot of the expression of thousands of genes in parallel and generating a large amount of data that can potentially provide systems-level information regarding the underlying mechanisms [34]. To test the hypothesis, we orally exposed BALB/c mice to PFOS at levels ranging from 0 to 210 mg/kg total administered dose (TAD), which was comparable to that utilized by Wang et al [33] and Zheng et al [19] (0e280 mg/kg TAD), for a period of three weeks, which was followed by one week of recovery. The one week recovery was set to observe the reversibility, retardance or incubation period for PFOS-induced immunotoxic effects, as PFOS was shown to bio-accumulate/persist in living bodies.…”

Section: Introductionmentioning

confidence: 99%

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In vivo immunotoxicity of perfluorooctane sulfonate in BALB/c mice: Identification of T-cell receptor and calcium-mediated signaling pathway disruption through gene expression profiling of the spleen

Wan

Guo

et al. 2015

Chemico-Biological Interactions

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a b s t r a c tPerfluorooctane sulfonate (PFOS) is a persistent organic pollutant that is used worldwide and is continuously being detected in biota and the environment, thus presenting potential threats to the ecosystem and human health. Although PFOS is highly immunotoxic, its underlying molecular mechanisms remain largely unknown. The present study examined PFOS-induced immunotoxicity in the mouse spleen and explored its underlying mechanisms by gene expression profiling. Oral exposure of male BALB/c mice for three weeks followed by one-week recovery showed that a 10 mg/kg/day PFOS exposure damaged the splenic architecture, inhibited T-cell proliferation in response to mitogen, and increased the percentages of T helper (CD3 þ CD4 þ ) and cytotoxic T (CD3 þ CD8 þ ) cells, despite the decrease in the absolute number of these cells. A delayed type of PFOS immunotoxicity was observed, which mainly occurred during the recovery period. Global gene expression profiling of mouse spleens and QRT-PCR analyses suggest that PFOS inhibited the expression of genes involved in cell cycle regulation and NRF2-mediated oxidative stress response, and upregulated those in TCR signaling, calcium signaling, and p38/MAPK signaling pathways. Western blot analysis confirmed that the expressions of CAMK4, THEMIS, and CD3G, which were involved in the upregulated pathways, were induced upon PFOS exposure. Acute PFOS exposure modulated calcium homoeostasis in splenocytes. These results indicate that PFOS exposure can activate TCR signaling and calcium ion influx, which provides a clue for the potential mechanism of PFOS immunotoxicity. The altered signaling pathways by PFOS treatment as revealed in the present study might facilitate in better understanding PFOS immunotoxicity and explain the association between immune disease and PFOS exposure.

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Fast ultrasound‐assisted extraction combined with LC–MS/MS of perfluorinated compounds in manure

García-Valcárcel

Tadeo

2013

J of Separation Science

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A fast analytical method for the determination of perfluorinated compounds in poultry manure by LC-MS/MS was developed. The extraction was carried out by ultrasound-assisted extraction of 1 g of sample, during 2 × 15 min using low volume (5.5 mL) of a mixture of methanol and acetonitrile. An efficient extraction of perfluoroalkyl carboxylates, perfluoroalkyl sulfonates, and perfluoroalkyl sulfonamides from poultry manure was obtained with recoveries higher than 81%. The cleanup of extracts was carried out by dispersive SPE. The validation of the proposed method showed the suitability of this procedure to determine perfluorinated compounds in poultry manure with detection limits in the range of 0.44-2.12 ng/g, depending on the target compound. In comparison with previously published methods, the miniaturization of the sample preparation method with ultrasound-assisted extraction together with the use of a core-shell column permit a lower consumption of organic solvents and a fast analysis of perfluorinated compounds. Manure samples obtained from Spanish commercial farms were analyzed and low perfluorinated compounds levels were found, which may be originated by dietary or environmental exposure. The highest concentrations measured corresponded to the perfluoroalkyl sulfonates, which varied from 8.2 to 35.9 ng/g.

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Modulation of dietary fat on the toxicological effects in thymus and spleen in BALB/c mice exposed to perfluorooctane sulfonate

Cited by 26 publications

References 34 publications

Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

Mechanism of perfluorooctanesulfonate (PFOS)-induced apoptosis in the immunocyte

In vivo immunotoxicity of perfluorooctane sulfonate in BALB/c mice: Identification of T-cell receptor and calcium-mediated signaling pathway disruption through gene expression profiling of the spleen

Fast ultrasound‐assisted extraction combined with LC–MS/MS of perfluorinated compounds in manure

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