Modulation of electroosmotic flow in capillary electrophoresis by plant polyphenol-inspired gallic acid/polyethyleneimine coatings: Analysis of small molecules

Zhang, Hao; Zhou, Dong‐Dong; Yang, Feng‐Qing; Qian, Zhengming; Li, Chunhong; Li, Wenjia; Wang, Shengpeng; Wang, Ying

doi:10.1016/j.jchromb.2019.05.031

Cited by 13 publications

(6 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the molecular weight of PEI increases from 600 to 70 000 Da, the structure of PEI molecular changes from a linear structure to a highly branched macromolecular structure. Meanwhile, the higher PEI molecular weight slowed down the interfacial polymerization rate and made the PA layer looser [57][58][59].…”

Section: Morphologies and Structuresmentioning

confidence: 99%

Analysis of Mg²⁺/Li⁺ separation mechanism by charged nanofiltration membranes: visual simulation

Guo

Shi

et al. 2020

Nanotechnology

View full text Add to dashboard Cite

The mechanism of the nanofiltration (NF) membrane separation of Mg2+ and Li+ needs to be further investigated, but some commonly used model theories are abstract, which makes them difficult to understand. More importantly, the relationship between the membrane charge and separation performance of Mg2+ and Li+ cannot be quantitatively analyzed. It is worth studying these challenges and providing a performance boost for Mg2+/Li+ filtration applications of NF membranes. Here, various NF membranes, with the membrane volumetric charge density increasing from −4.69 to 7.02 mol · m−3, were fabricated via interfacial polymerization. For these membranes, the separation factor S Mg,Li was decreased from 0.41 to 0.20. Importantly, the visual simulation results were consistent with the experimental results as a whole. The separation factor S Mg,Li decreased with the increase of volumetric charge density, and the minimum separation factor S Mg,Li of the NF membranes was 0.20 (experiment) and 0.17 (simulation), respectively. This meant that the performance of the positively charged NF membrane was not fully developed. Furthermore, we analyzed the relationship between the membrane charge and separation performance, and visualized the simulation of the NF membrane filtration and separation.

show abstract

Section: Morphologies and Structuresmentioning

confidence: 99%

Analysis of Mg²⁺/Li⁺ separation mechanism by charged nanofiltration membranes: visual simulation

Guo

Shi

et al. 2020

Nanotechnology

View full text Add to dashboard Cite

show abstract

“…To control the velocity and direction of EOF in CE, different techniques involving capillary inner surface modification have been introduced, such as dynamic coating, physical adsorption, and covalent bonding methods [3,[7][8][9][10][11]. The dynamic coating method has gained distinction for its simplicity and practicality.…”

Section: Introductionmentioning

confidence: 99%

Sulfonic Functionalized Polydopamine Coatings with pH-Independent Surface Charge for Optimizing Capillary Electrophoretic Separations

Long,

You,

et al. 2024

Molecules

View full text Add to dashboard Cite

Enhancing the pH-independence and controlling the magnitude of electroosmotic flow (EOF) are critical for highly efficient and reproducible capillary electrophoresis (CE) separations. Herein, we present a novel capillary modification method utilizing sulfonated periodate-induced polydopamine (SPD) coating to achieve pH-independent and highly reproducible cathodic EOF in CE. The SPD-coated capillaries were obtained through post-sulfonation treatment of periodate-induced PDA (PDA-SP) coatings adhered on the capillary inner surface. The successful immobilization of the SPD coating and the substantial grafting of sulfonic acid groups were confirmed by a series of characterization techniques. The excellent capability of PDA-SP@capillary in masking silanol groups and maintaining a highly robust EOF mobility was verified. Additionally, the parameters of sulfonation affecting the EOF mobilities were thoroughly examined. The obtained optimum SPD-coated column offered the anticipated highly pH-independent and high-strength cathodic EOF, which is essential for enhancing the CE separation performance and improving analysis efficiency. Consequently, the developed SPD-coated capillaries enabled successful high-efficiency separation of aromatic acids and nucleosides and rapid cyclodextrin-based chiral analysis of racemic drugs. Moreover, the SPD-coated columns exhibited a long lifetime and demonstrated good intra-day, inter-day, and column-to-column repeatability.

show abstract

“…To tackle this limitation, the physicochemical modification of the capillary nature is often used as it prevents nonspecific adsorption of proteins and can advantageously be used to control the EOF in capillary zone electrophoresis (CZE). The CZE separation, which is governed by EOF and effective electrophoretic mobilities ( µ ep ) of analytes, is strongly affected by EOF modulation, which contributes to improve selectivity [4]. Moreover, the coating on the inner wall of the capillary column can allow to generate analyte–surface interactions (chromatographic retention) and help in the separation of analytes.…”

Section: Introductionmentioning

confidence: 99%

A bioinspired approach for the modulation of electroosmotic flow and protein–surface interactions in capillary electrophoresis using silylated amino‐amides blocks and covalent grafting

Gouyon,

Clavié,

Raquel

et al. 2023

Electrophoresis

View full text Add to dashboard Cite

We explore a bioinspired approach to design tailored functionalized capillary electrophoresis (CE) surfaces based on covalent grafting for biomolecules analysis. First, the approach aims to overcome well‐known common obstacles in CE protein analysis affecting considerably the CE performance (asymmetry, resolution, and repeatability) such as the unspecific adsorption on fused silica surface and the lack of control of electroosmotic flow (EOF). Then, our approach, which relies on new amino‐amide mimic hybrid precursors synthesized by silylation of amino‐amides (Si–AA) derivatives with 3‐isocyanatopropyltriethoxysilane, aims to recapitulate the diversity of protein–protein interactions (π–π stacking, ionic, Van der Waals…) found in physiological condition (bioinspired approach) to improve the performance of CE protein analysis (electrochromatography). As a proof of concept, these silylated Si–AA (tyrosinamide silylation, serinamide silylation, argininamide silylation, leucinamide silylation, and isoglutamine silylation acid) have been covalently grafted in physiological conditions in different amount on bare fused silica capillary giving rise to a biomimetic coating and allowing both the modulation of EOF and protein–surface interactions. The analytical performances of amino‐amide functionalized capillaries were assessed using lysozyme, cytochrome C and ribonuclease A and compared to traditional capillary coatings poly(ethylene oxide), poly(diallyldimethylammonium chloride), and sodium poly(styrenesulfonate). EOF, protein adsorption rate, protein retention factor k, and selectivity were determined for each coating. All results obtained showed this approach allowed to modulate the EOF, reduce unspecific adsorption, and generate specific interactions with proteins by varying the nature and the amount of Si–AA in the functionalization mixture.

show abstract

Modulation of electroosmotic flow in capillary electrophoresis by plant polyphenol-inspired gallic acid/polyethyleneimine coatings: Analysis of small molecules

Cited by 13 publications

References 58 publications

Analysis of Mg²⁺/Li⁺ separation mechanism by charged nanofiltration membranes: visual simulation

Analysis of Mg²⁺/Li⁺ separation mechanism by charged nanofiltration membranes: visual simulation

Sulfonic Functionalized Polydopamine Coatings with pH-Independent Surface Charge for Optimizing Capillary Electrophoretic Separations

A bioinspired approach for the modulation of electroosmotic flow and protein–surface interactions in capillary electrophoresis using silylated amino‐amides blocks and covalent grafting

Contact Info

Product

Resources

About

Modulation of electroosmotic flow in capillary electrophoresis by plant polyphenol-inspired gallic acid/polyethyleneimine coatings: Analysis of small molecules

Cited by 13 publications

References 58 publications

Analysis of Mg2+/Li+ separation mechanism by charged nanofiltration membranes: visual simulation

Analysis of Mg2+/Li+ separation mechanism by charged nanofiltration membranes: visual simulation

Sulfonic Functionalized Polydopamine Coatings with pH-Independent Surface Charge for Optimizing Capillary Electrophoretic Separations

A bioinspired approach for the modulation of electroosmotic flow and protein–surface interactions in capillary electrophoresis using silylated amino‐amides blocks and covalent grafting

Contact Info

Product

Resources

About

Analysis of Mg²⁺/Li⁺ separation mechanism by charged nanofiltration membranes: visual simulation

Analysis of Mg²⁺/Li⁺ separation mechanism by charged nanofiltration membranes: visual simulation